Additional file 3: of Activity-dependent decrease in contact areas between subsurface cisterns and plasma membrane of hippocampal neurons

Number of subsurface cistern (SSC) normalized to per 10 pyramidal neuronal somas in the CA1 region of the hippocampus in organotypic slice cultures. (PDF 264 kb

Additional file 5: of Activity-dependent decrease in contact areas between subsurface cisterns and plasma membrane of hippocampal neurons

Percentages of each of the three types of SSC (A, B and C) in neuronal somas before and after depolarization. (PDF 264 kb

Additional file 4: of Activity-dependent decrease in contact areas between subsurface cisterns and plasma membrane of hippocampal neurons

Average length of subsurface cistern (mean ± SEM in nm) in pyramidal neuronal somas in the CA1 region of organotypic hippocampal slice cultures. (PDF 45 kb

Additional file 1: of Activity-dependent decrease in contact areas between subsurface cisterns and plasma membrane of hippocampal neurons

Number of subsurface cisterns (SSC) normalized to per 10 neuronal somas in dissociated hippocampal cultures. (PDF 264 kb

Additional file 3: of Stimulation-induced structural changes at the nucleus, endoplasmic reticulum and mitochondria of hippocampal neurons

Structural differences between ER lamellar bodies and ER cisternal stacks. (PDF 3994 kb

Additional file 1: of Stimulation-induced structural changes at the nucleus, endoplasmic reticulum and mitochondria of hippocampal neurons

Neural chromatin clustering upon stimulation. (PDF 2492 kb

Additional file 2: of Stimulation-induced structural changes at the nucleus, endoplasmic reticulum and mitochondria of hippocampal neurons

Chromatin clustered upon depolarization in neurons but not in astrocytes. (PDF 5705 kb

Additional file 6: of Activity-dependent decrease in contact areas between subsurface cisterns and plasma membrane of hippocampal neurons

(A) Total number of SSC and (B) Subtotal number of SSC with a flattened stack of ER in pyramidal neuronal somas in the CA1 region of hippocampal slice cultures. Number of SSC in control samples normalized to per 10 neuronal somas. Values for other conditions normalized to % of controls. (PDF 52 kb

Additional file 2: of Activity-dependent decrease in contact areas between subsurface cisterns and plasma membrane of hippocampal neurons

Average length of subsurface cistern (mean ± SEM in nm) in neuronal somas of dissociated hippocampal neuronal cultures. (PDF 46 kb

Some cell adhesion molecules are enriched in the SJ fraction, suggesting selective localization at the synaptic cleft.

<p>Western immunoblots comparing synaptic junction (SJ) fractions and parent homogenate (H) and synaptic plasma membrane (SPM) fractions. The lower portion of neurexin immunoblot and the upper portion of SynCAM 1/2/3 immunoblot correspond to higher exposure times to allow optimal visualization of all isoforms. Certain proteins including neuroligins, neurexins, N-cadherin, SynCAM 1 and SALM5 show distinctive enrichment. Equal amounts of protein were loaded into each lane. Experiments were repeated at least twice using different SJ fractions, with similar results (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0174895#pone.0174895.s002" target="_blank">S2 Fig</a>).</p