PacSNP chum data for MER

The file is formatted for GenAlEx software, but can easily be converted to Genepop or similar formats. Genotypes are in two-column format with missing data designated with a “0”. Population code and locus code worksheets follow the rawdata. The population order differs between the rawdata which is in numeric order and Table 1 of the manuscript which is ordered geographically. This is particularly noticeable in the order of the Western Alaska populations including the Yukon River. The rawdata includes laboratory names for the SNPs; a key to published names is in the locus code worksheet. The mtDNA SNPs are listed independently and have not been combined into composite haplotypes

Multifunctional Polyurethane Sponge for Polymerase Chain Reaction Enhancement

Selective filtering of target biomaterials from impurities is an important task in DNA amplification through polymerase chain reaction (PCR) enhancement and gene identification to save endangered animals and marine species. Conventional gene extraction methods require complicated steps, skilled persons, and expensive chemicals and instruments to improve DNA amplification. Herein, we proposed an alternative method for overcoming such challenges by imparting secondary functionality using commercially available polyurethane (PU) sponges and cost-effective fabrication approaches through polydopamine and polysiloxane coatings. The porous, highly flexible, and chemically modified superhydrophilic and superhydrophobic PU sponges allow large surface areas and mechanically stable frames for effective extraction of genomic DNA through selective filtering of fish tissues and oils. Furthermore, these chemically modified PU sponges allow separation of genes and improvement of PCR for DNA amplification for the identification of fish species. The combination of a simple fabrication method and functionalized PU sponges could be a useful platform for PCR enhancement and gene-based identification of species for practical applications