Histomorphological Effect of Centella Asiatica on Swiss Albino Mice Brain upon Ketamine Anesthetic Induced Memory Impairment

Introduction: Ketamine is a medication mainly used for starting and maintaining anesthesia. It induces a trance-like state while providing pain relief, sedation and memory loss. Ketamine causes morphological changes in the neuronal cells of growing rodent. Centella asiatica is used for memory enhancement in the ayurvadic system of medicine. It has been shown to be useful in improving learning and memory. Centella asiatica leaf extract treatment enhances neuronal dendritic  arborization in hippocampus of growing rodent.Materials and Methods: Swiss albino mice were used for our experiment. Ketamine was administered subcutaneously and Centella-asiatica leaf extract was administered orally. Mice brain collected, grossed, processed and finally stained by Hematoxylin and Eosin (H&E) staining process for viewing cellular and tissue structure detail by pathologists. Results: Focal cellular damage was seen in CA2 and CA4 area of cornu ammonis (CA), expanded cytoplasmic eosinophilia saw in the pyramidal cell layers (PY) of CA2, expanded cytoplasmic eosinophilia found with obliteration of neurifibrilary components (NFE), typical pyramidal cell (NPY) in the CA4, ordinary engineering of dentate granule layers (DG) and hardly any cells were found with diminished size in regards to granule layer cells (GC) in the brain hippocampus of the high ketamine prompted mice. In the mouse brain hippocampus for high ketamine-Centella asiatica summed up cell destruction found in the CA1 to CA4 of CA. Expanded cytoplasmic eosinophilia found in the pyramidal cell layers of CA2 than high dose ketamine group. The vast majority of the pyramidal cells were necrotic (PYN) with annihilation of neurofibrilary components (NFE) in the pyramidal cell layers of CA4. Keywords: Centella asiatica, Ketamine and memory impairment. DOI: 10.7176/ALST/82-02 Publication date:October 31st 202

Impact of Lymphatic Filariasis (LF) on Hemoglobin Content and Anemia: A Cross-Sectional Based Study

Lymphatic filariasis (LF) is a vector-borne chronically disabling parasitic infection causing elephantiasis, lymphedema, and hydrocele. The infection is endemic in 83 countries worldwide, with more than 1.2 billion people at risk and 120 million already infected. LF is caused by thread-like adult parasitic worms, which live in the human lymphatic system and causes swelling of the scrotum, male genitalia, breast and limbs. It is caused by tissue-dwelling nematode worms belonging to the order “Filariidea” which is transmitted to man by the infective bites of blood sucking mosquitoes. About 90% of these infections are caused by Wuchereria bancrofti.  There are limited number of reports available describing the presence of microfilaria in bone marrow. There is a report a young patient who developed aplastic anemia following varicella infection, and peripheral blood and bone marrow showed many microfilariae of Wuchereria bancrofti. This experiment was designed to assess hemoglobin content by sex; and anemic condition by age and sex among 119 case respondents and 102 controls.  Hemoglobin level was found to be a bit better among case respondents than controls. By sex, hemoglobin level was better among males than females in both case respondents and control groups. Among case respondents 6-59 months age male group and 5-9 years age female group was found anemic. But in control group all the male respondents in all age groups and only one female in 5-9 years age group were found anemic. Keywords: Hemoglobin, Anemia and Lymphatic Filariasis

Amoxyclav Resistance Pattern and Aerobic Bacterial Profile in Diabetic Foot Infection Patients in Bangladesh

Introduction: The goal of this study was to determine the frequency of bacterial isolates cultured from diabetic foot infections and to assess their amoxyclav resistance and susceptibility.Methods: A total of 378 diabetic foot lesions were included in this prospective analysis. The antibiotic susceptibility pattern of bacteria isolated from foot lesions was assessed using the Kirby-Bauer disk diffusion method.Results: The most commonly isolated Gram-positive bacteria were Staphylococcus aureus, followed by Enterococcus spp. and CoNS. The most commonly isolated Gram-negative bacteria were Klebsiella spp., Pseudomonas spp., Proteus spp., Escherichia coli, Enterobacter spp., Citrobacter spp., Citrobacter freundii, and Proteus vulgaris. Amoxyclav was found to be 100.00% resistant against Pseudomonas aeruginosa and followed by Enterococcus spp. (89.50%), Proteus spp. (87.50%), Staphylococcus aureus (84.30%), Escherichia coli (81.50%), Klebsiella spp. (70.50%) and Enterobacter spp. (69.20%).Conclusion: The present study confirmed the prevalence of amoxyclav drug resistant pathogens (84.90%) in diabetic foot ulcers. The diverse bacteria infecting the wound must be evaluated, as well as the antibiotic susceptibility patterns of the isolates from the infected lesion. This information is critical for selecting the right medications, eliminating resistance trends, and lowering healthcare costs. Keywords: Diabetic Foot Infection, Polymicrobial Infections, Amoxyclav DOI: 10.7176/JHMN/94-03 Publication date:October 31st 202

Successful Transplantation of Primary Hepatocytes from DsRed Mice into Fah-/-;Scid/Scid Mice; A Future In Vivo Model System for Receiving Human Hepatocytes

We have developed a system for studying hepatocellular growth potential in which liver cells are introduced into the diseased livers of Fah-/-; Scid/Scid double knockout mice. To use this system to study cell transplantation, DsRed  liver cells were introduced into severe immunodeficient Fah-/-; Scid/Scid double knockout  mice. In regenerated recipient livers, up to 20% of the mouse liver is repopulated by DsRed mouse hepatocytes demonstrating the creation of a functional mouse liver in which parenchyma is derived from DsRed mouse hepatocytes. The severe immunodeficient Fah-/-;Scid/Scid double knockout mice provide a tool for studying hepatocellular biology. Keywords: Human Hepatocytes, Transplantation, DsRed and Fah-/-;Scid/Scid Mice

Resistance Pattern of Levofloxacin against Uropathogens Causing Urinary Tract Infection in Selected Areas of Dhaka city, Bangladesh

A crucial public health problem in developing country like Bangladesh is resistance of antibiotics to different types of bacteria and the rates of these bacterial resistances are changing for various antibiotic therapy. Our aim was to assess the susceptible pattern of Levofloxacin a 3rd generation Quinolone antibiotic against uropathogens. A total of 12943 urine samples were collected in 2016 (Jan-Dec) and out of which 1236 (9.55%) were bacteriologically positive. Among the isolated uropathogens, 95.1% were gram negative and 4.9% gram positive organism. Male were found more prone to get UTI under 10 years and between 51-90 years of age and female were more affected in 10 to 50 years and over 90 years of age group. E. coli was the most prevalent (83.9%) isolate followed by Klebsiella spp. (6.7%), Staphylococcus aureus (2.6%), Pseudomonas spp. (2.2%), Enterococcus spp. (2.0%) and Proteus spp. (1.1%). The most predominant Levofloxacin sensitive organisms were found in male Enterobacter spp. (100%) and in female patients Serretia spp. (100%), Citrobacter spp. (100%) and Streptococcus Group B (100%). On the other hand the most predominant Levofloxacin resistant organisms were found in male and female both Acinetobacter spp. (100%). Around (61.7%) male and (46.9%) female were found resistant to E. coli. Keywords: Levofloxacin, Quinolone, UTI, Resistance, Uropathogen

Iron, TIBC and Ferritin Levels Correlation with Testosterone Deficient Males in Dhaka, Bangladesh

Low serum iron and ferritin levels with an elevated TIBC are diagnostic of iron deficiency. While a low serum ferritin is virtually diagnostic of iron deficiency, a normal serum ferritin can be seen in patients who are deficient in iron and have coexistent diseases (eg, hepatitis or anemia of chronic disorders). Men with low testosterone levels have lower hemoglobin and hematocrit levels, while men with anemia of unknown etiology. Testosterone directly regulates body iron levels through inhibition of the master regulator of iron metabolism, hepcidin. In our study we studied with 30 males. 80.0% males were observed with low iron level, 100.0% found with normal ferritin level, 66.7% with normal TIBC and finally we found 96.7% males with low serum testosterone level. Keywords: Iron, TIBC, ferritin and testosterone. DOI: 10.7176/JHMN/81-10 Publication date:October 31st 202

Minerals, Toxic Heavy Metals, and Antioxidant Properties of Honeys from Bangladesh

The study reports on major and trace elements as well as antioxidant properties of honey samples from Bangladesh. Four major cationic elements, seven trace elements, and three heavy metals were determined in the 12 honey samples using atomic absorption spectrophotometer. Nutritional values in these honey samples were further investigated according to their antioxidant properties. The content of major elements was in the range of 62.75–616.58, 579.48–2219.43, 69.42–632.25, and 0.13–1.20mg/kg for sodium, potassium, magnesium, and calcium, respectively. The trace elements varied in the range of 0.41–28, 0.12–3.54, 1.54–2.85, 0.29– 0.59, 0.02–0.35, and 0.01–0.06mg/kg for iron, zinc, copper, nickel, cobalt, and cadmium, respectively. Among the heavy metals, only lead (0.17–2.19 mg/kg) was detected.The results of antioxidant analysis based on phenolics, flavonoids, ascorbic acid, reducing sugar, and proteins (as nonphenolic antioxidants) revealed that multifloral raw honey samples contain significantly higher levels of reducing agents than monofloral and commercial brand honeys. The study provides a useful insight on the minerals, heavy metals, and antioxidant properties of honey samples commonly consumed in Bangladesh and found to be rich source of antioxidants and minerals. Some samples might pose some risk to the health due to lead contamination

Evaluation of Gentamicin's Antimicrobial Resistance Pattern Against Uropathogens in a Tertiary Health Care Center in Dhaka City, Bangladesh

Treatment of UTI patients with aminoglycosides is a familiar incidence. Resistance of antibiotics is also a familiar incidence but it is alarming when it goes to a high ratio. The vital public health problem in developing country like Bangladesh is resistance of antibiotics to different types of bacteria causing UTI and the rates of these bacterial resistances are changing for various antibiotic therapy. Our aim was to assess the susceptible pattern of Gentamicin a drug of Aminoglycosides group against uropathogens. A total of 12943 urine samples were collected in 2016 (Jan-Dec) and out of which 1236 (9.55%) were bacteriologically positive out of these isolated  95.1% were gram negative and 4.9% gram positive organism. Male were found more prone to get UTI under 10 years and between 51-90 years of age and female were more affected in 10 to 50 years and over 90 years of age group. E. coli was the most prevalent (83.9%) isolate followed by Klebsiella spp. (6.7%), Staphylococcus aureus (2.6%), Pseudomonas spp. (2.2%), Enterococcus spp. (2.0%) and Proteus spp. (1.1%). The most predominant organism Acinetobacter spp. (100%) were found sensitive to Gentamicin in both male and female patients and Enterococcus spp. in male (62.5%) and female (58.8%) were found resistant.  Around 37.4% male and 32.5% female were found resistant to E.coli. Keywords: Gentamicin, Aminoglycosides, UTI, Resistance, Uropathogen. DOI: 10.7176/JHMN/67-08 Publication date:October 31st 201

Minerals, Toxic Heavy Metals, and Antioxidant Properties of Honeys from Bangladesh

The study reports on major and trace elements as well as antioxidant properties of honey samples from Bangladesh. Four major cationic elements, seven trace elements, and three heavy metals were determined in the 12 honey samples using atomic absorption spectrophotometer. Nutritional values in these honey samples were further investigated according to their antioxidant properties. The content of major elements was in the range of 62. 75-616.58, 579.48-2219.43, 69.42-632.25, and 0.13-1.20 mg/kg for sodium, potassium, magnesium, and calcium, respectively. The trace elements varied in the range of 0. 41-28, 0.12-3.54, 1.54-2.85, 0.29-0.59, 0.02-0.35, and 0.01-0.06 mg/kg for iron, zinc, copper, nickel, cobalt, and cadmium, respectively. Among the heavy metals, only lead (0.17-2.19 mg/kg) was detected. The results of antioxidant analysis based on phenolics, flavonoids, ascorbic acid, reducing sugar, and proteins (as nonphenolic antioxidants) revealed that multifloral raw honey samples contain significantly higher levels of reducing agents than monofloral and commercial brand honeys. The study provides a useful insight on the minerals, heavy metals, and antioxidant properties of honey samples commonly consumed in Bangladesh and found to be rich source of antioxidants and minerals. Some samples might pose some risk to the health due to lead contamination

Exploring the impact of culture media on life history characteristics of the house fly, Musca domestica L. (Diptera: Muscidae)

Insects, particularly house fly pupae (M. domestica L.), are increasingly promoted as a rich and sustainable protein source, presenting a practical solution for organic waste management. These insects possess the capability to transform animal waste into valuable biomass, underscoring the necessity to evaluate housefly larval growth on diverse waste mixtures. The primary objective was to comprehend how different culture media influence the life history traits of this species. To address this, the research aimed to assess the growth and development performance of housefly larvae reared on various waste mixtures. Adult house flies (M. domestica) were sourced from meat and fish markets in Rajshahi city, Bangladesh, employing systematic sampling from different market sections for a representative selection. Experimental diets were formulated with combinations of cattle blood, cattle manure, rice bran, wheat bran, and kitchen waste, categorized into Diet-A (kitchen waste:cattle manure:wheat bran), Diet-B (cattle blood:rice bran), Diet-C (cattle manure:wheat bran), and Diet-D (cattle blood:kitchen waste:rice bran). Additionally, a control group comprised a mixture of waste materials, including yeast, egg white, milk powder, and wheat bran. The findings revealed that larvae, pupae, and adults reared on Diet-B, incorporating cattle blood and rice bran, demonstrated increased length compared to other diets. This varied diet also produced the heaviest larvae, pupae, and adults, exhibiting the highest morphometric parameters. The moisture content of pupae varied across the different diets, ranging from 53.23±0.17 % to 65.47±1.32 %. The pupae reared on Diet-B had the highest levels of crude protein (53.44±0.05 %, p < 0.05), while those reared on Diet-C had the highest levels of fat (18.56±0.05 %, p < 0.05 except for diet A). The findings demonstrate that housefly larvae can be reared on diets containing rice bran and cattle blood, which have different nutritional profiles and biological capacities. However, the ideal rearing strategy should depend on how M. domestica will be utilized in the future. The study highlights insects, especially housefly pupae, as a sustainable protein source and an organic waste management solution. Therefore, the results of this study contribute to the broader understanding of Musca domestica and offer practical implications for both scientific research and pest management practices