Additional file 4: of Adaptations in energy metabolism and gene family expansions revealed by comparative transcriptomics of three Chagas disease triatomine vectors

Transcripts coding for odorant receptors and odorant binding proteins in Triatoma. (XLS 78 kb

Additional file 7: of Adaptations in energy metabolism and gene family expansions revealed by comparative transcriptomics of three Chagas disease triatomine vectors

Gene ontology term enrichment in divergent vs. conserved group putative 1:1 orthologs (isotig dataset). (XLS 43 kb

Additional file 5: of Adaptations in energy metabolism and gene family expansions revealed by comparative transcriptomics of three Chagas disease triatomine vectors

Protein sequence divergence in putative 1:1 orthologs according to Gene Ontology terms (Level 3). Isotig dataset. Data for Fig. 5. (XLS 54 kb

Additional file 2: of Adaptations in energy metabolism and gene family expansions revealed by comparative transcriptomics of three Chagas disease triatomine vectors

Analysis of the calycin family in Triatoma. Data for Fig. 3. (XLS 58 kb

Socio-demographic indicators, symptoms and co-morbidities of the sampled population.

*<p><b><i>X²</i></b> test.</p

Oligonucleotide sequences used in this study.

*<p>Underlined nucleotides indicate the stop codon for mutagenesis in positions 823–825.</p><p>AdaptAFlx refers to the 454 adaptor A sequence. AdaptAFlx refers to the 454 adaptor B sequence. 454 key indicates 454 library key sequence (TCAG). Nucleotides in bold italics indicate barcodes. Regular font indicates NA gene specific sequence (699–716; 983–1000).</p

Sequence coverage plots of the sequenced NA amplicon according to barcode (A) and according to picotiterplate region (B).

<p>Positions of the NA gene are shown in X axis, whereas the number of reads is shown in Y axis. Less variability in read number was obtained according to MID than according to sequencing region, suggesting that the source of variability is during the emPCR amplification.</p

Overall metrics and results of the sequencing run.

<p>Na. Not applicable.</p

Genetic diversity of Influenza A(H1N1)pdm virus in the State of Morelos (2009).

<p>The graph shows the proportion (Y axis) of each of the four genetic groups described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0067010#pone-0067010-t003" target="_blank"><b>Table 3</b></a> in each of the 48 libraries, (X axis) ranked according to epidemiological week of the latest individual in the pool. Thus, the earliest cases are seen in the left, whereas the later cases are shown at right. The red dots represent the haplotypes of group IVb, i.e. those haplotypes that contained additional non-synonymous mutations to D248N. Black arrows in the top represent libraries in which individual amplicons were validated by Sanger sequencing. The asterisk (*) indicates those libraries which included an individual from the first wave.</p

Influenza A (H1N1)pdm NA gene variants identified.

<p>Bold italics: Mutations associated with oseltamivir resistance.</p>&<p>Rounded quotient of the proportional frequency of the haplotype within each barcoded library divided by the actual number (corrected) of amplicon in each barcoded library.</p