Genome map of phage HY01.

<p>Genome map of phage HY01.</p

Comparative genomic analysis of phage HY01 with two closely related <i>E</i>. <i>coli</i> phage genomes (AR1 and T4).

<p>(A) Whole genome alignment of three phage genomes using ACT13. The yellow open box indicates a small gene cluster with low homology between the HY01 and T4 genomes. (B) Organization of a small gene cluster from the yellow open box. The white arrows indicate long tail fiber-associated genes, the blue arrows indicate receptor recognition genes, and the black arrows indicate genes with other functions.</p

General genome features of phage HY01 and other closely related phages.

<p>General genome features of phage HY01 and other closely related phages.</p

Bacterial challenge assay of phage HY01.

<p>(A) <i>E</i>. <i>coli</i> O157:H7 ATCC 43890 and (B) <i>S</i>. <i>flexneri</i> 2a strain 2457T as host strains. Open circles, HY01-infected samples; closed circles, non-infected samples.</p

MOESM1 of The complete genome sequence of Cronobacter sakazakii ATCC 29544T, a food-borne pathogen, isolated from a child’s throat

Additional file 1: Figure S1. The morphology of C. sakazakii ATCC 29544 imaged by energy-filtering transmission electron microscopy (EF-TEM). EF-TEM photograph was obtained by 2% uranyl acetate on copper grids and examined with ET-TEM at a voltage of 120 kV (LIBRA 120, Zeiss, Oberkochen, Germany). Figure S2: Comparative analysis of two ATCC 29544-specific gene clusters: (A) lac operon and (B) arsenic resistance. The amino acid sequence identities between associated genes are indicated as percentages

Comparative genome analysis of phage HY01 and 19 closely related or similar phages.

<p>(A) Dot plot alignment of 20 phage genome sequences. 1. Phage HY01, 2. <i>E</i>. <i>coli</i> phage RB51 (FJ839693), 3. <i>E</i>. <i>coli</i> O157:H7 phage ECML-134 (JX128259), 4. <i>E</i>. <i>coli</i> O157:H7 phage AR1 (AP011113), 5. <i>Yersinia</i> phage PST (KF208315), 6. <i>S</i>. <i>flexneri</i> phage Shfl2 (HM035025), 7. <i>E</i>. <i>coli</i> phage T4 (AF158101), 8. <i>E</i>. <i>coli</i> phage ime09 (JN202312), 9. <i>E</i>. <i>coli</i> phage RB32 (DQ904452), 10. <i>E</i>. <i>coli</i> O157:H7 phage wV7 (HM997020), 11. <i>Cronobacter sakazakii</i> phage vB_CsaM_GAP161 (JN882287), 12. <i>Klebsiella pneumoniae</i> phage KP27 (HQ918180), 13. <i>Serratia</i> sp. phage phiMAM1 (JX878496), 14. <i>Campylobacter jejuni</i> phage CPX (JN132397), 15. <i>E</i>. <i>coli</i> O157:H7 phage bV_EcoS_AKFV33 (HQ665011), 16. <i>E</i>. <i>coli</i> phage HK106 (JQ086369), 17. <i>E</i>. <i>coli</i> phage mEp234 (JQ182732), 18. <i>Bacillus cereus</i> phage Bastille (JF966203), 19. <i>E</i>. <i>coli</i> phage vB_EcoM-FV3 (JQ031132), 20. <i>Salmonella</i> Enteritidis phage PVP-SE1 (GU070616). (B) Phylogenetic tree analysis of terminase large subunits from phage HY01 and 19 phages using the neighbor-joining method. Bootstrap values are indicated at the branches of the tree. Group I phages are indicated with a bracket.</p

Host range of phage HY01.

<p>Host range of phage HY01.</p

Food application of phage HY01.

<p>(A) <i>E</i>. <i>coli</i> O157:H7 ATCC 43890 (clinical isolate) and (B) <i>E</i>. <i>coli</i> O157:H7 ATCC 43895 (food isolate) as host strains. Closed circles, control sample without HY01; closed squares, MOI = 10⁵; open squares, MOI = 10⁶.</p

Comparative genomic analysis of phage HY01 with two closely related <i>Shigella</i> phage genomes (pSs-1 and Shfl2).

<p>(A) Whole genome alignment of three phage genomes using ACT13. The yellow open box indicates a small gene cluster with low homology between the HY01 and Shfl2 genomes. (B) Organization of a small gene cluster from the yellow open box. The white arrows indicate long tail fiber-associated genes, the blue arrows indicate receptor recognition genes, and the black arrows indicate genes with other functions.</p

Comparative protein sequence analysis of host specificity-associated proteins in a phage HY01 gene cluster.

<p>Comparative protein sequence analysis of host specificity-associated proteins in a phage HY01 gene cluster.</p