2,950 research outputs found
Multivariate chromosome analysis and complete karyotyping using dual labeling and fluorescence digital imaging microscopy.
Heterogeneity of signal transduction at the subcellular level: microsphere-based focal EGF receptor activation and stimulation of Shc translocation
Epidermal growth factor receptor (EGFR, erbB1) activation and translocation of the Shc adaptor protein to activated receptors were analyzed at the subcellular level by dual-label immunofluorescence and confocal laser scanning microscopy in conjunction with a new microsphere-based protocol. In the Quantitative Microsphere Recruitment Assay (QMRA) introduced here, epidermal growth factor-coated 1 mm diameter microspheres were distributed over the surface of adherent tissue culture cells expressing the receptor. High-resolution confocal microscopy of a fusion construct of the receptor and the green fluorescent protein expressed in Chinese hamster ovary cells demonstrated that engulfment and internalization of the microspheres occurred rapidly within minutes, and in a receptor activation-dependent manner. In human epidermoid carcinoma A431 cells, receptor activation and Shc translocation persisted over the 20-minute time course of the experiments. However, at the subcellular level the positive correlation of receptor activation and Shc translocation observed at 5-8 minutes dissipated, indicating a time-dependent decoupling of thetwo events and variation in the kinetics of signal transduction for different subcellular locations
Biotin-ligand complexes with Streptavidin quantum dots for in vivo cell labeling of membrane receptors
Efficient superresolution restoration algorithms using maximum a posteriori estimations with application to fluorescence microscopy
Spectroscopic properties and helical stabilities of 25-nt parallel-stranded linear DNA duplexes.
Helix-coil transition of parallel-stranded DNA. Thermodynamics of hairpin and linear duplex oligonucleotides.
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