Additional file 1: of Changes in trust and the use of Korean medicine in South Korea: a comparison of surveys in 2011 and 2014

Questionnaire to investigate the attitude toward Korean Medicine among the public in South Korea (DOCX 21 kb

Additional file 3: of Changes in trust and the use of Korean medicine in South Korea: a comparison of surveys in 2011 and 2014

Table S1. Demographics of respondents and trust of KM and Western medicine in 2011 and 2014 (DOCX 37 kb

(A) Acrosome reaction of sperm from the epididymis and vas deferens was induced by calcium ionophore A23187

Acrosome-intact and -reacted sperm were subjected to western blot anaylsis with the anti-Shsp1/Mm.87328 antibody. AI, acrosome-intact sperm; AR, acrosome-reacted sperm. (B) Sperm from the epididymis and vas deferens were treated with 1% Triton X-100 and urea with different concentrations (2, 3, 4 and 6 M). Soluble and insoluble fractions after centrifugation of the treated sperm were subjected to western blot anaylsis with the anti-Sfap1/Mm.386907 and anti-Sfap2/Mm.157049 antibodies. Sup, supernatant after centrifugation; Pt, pellet after centrifugation.<p><b>Copyright information:</b></p><p>Taken from "Characterization of eight novel proteins with male germ cell-specific expression in mouse"</p><p>http://www.rbej.com/content/6/1/32</p><p>Reproductive biology and endocrinology : RB&E 2008;6():32-32.</p><p>Published online 24 Jul 2008</p><p>PMCID:PMC2500023.</p><p></p

Cells at different developmental stages were isolated, boiled in 3% SDS with 5% β-mercaptoethanol, subjected to SDS-PAGE and blotted with the specific antibodies

TC, testicular (spermatogenic) cells; TS, testicular sperm; S, mature sperm from the epididymis and vas deferens.<p><b>Copyright information:</b></p><p>Taken from "Characterization of eight novel proteins with male germ cell-specific expression in mouse"</p><p>http://www.rbej.com/content/6/1/32</p><p>Reproductive biology and endocrinology : RB&E 2008;6():32-32.</p><p>Published online 24 Jul 2008</p><p>PMCID:PMC2500023.</p><p></p

Testes from mutant mice (A) and a Sertoli cell line, TM4 (B), were subjected to protein extraction and western blot analysis using the specific antibodies

Total proteins from the testes were extracted by boiling the minced tissues in a protein sample buffer containing 3% SDS and 5% β-mercaptoethanol. ADAM2, with spermatogenic cell-specific expression, was included as a control. WT, wild type; TC, testicular (spermatogenic) cells.<p><b>Copyright information:</b></p><p>Taken from "Characterization of eight novel proteins with male germ cell-specific expression in mouse"</p><p>http://www.rbej.com/content/6/1/32</p><p>Reproductive biology and endocrinology : RB&E 2008;6():32-32.</p><p>Published online 24 Jul 2008</p><p>PMCID:PMC2500023.</p><p></p