1 research outputs found
Chronic Ethanol Consumption Profoundly Alters Regional Brain Ceramide and Sphingomyelin Content in Rodents
Ceramides
(CER) are involved in alcohol-induced neuroinflammation.
In a mouse model of chronic alcohol exposure, 16 CER and 18 sphingomyelin
(SM) concentrations from whole brain lipid extracts were measured
using electrospray mass spectrometry. All 18 CER concentrations in
alcohol exposed adults increased significantly (range: 25–607%);
in juveniles, 6 CER decreased (range: −9 to −37%). In
contrast, only three SM decreased in adult and one increased significantly
in juvenile. Next, regional identification at 50 μm spatial
resolution from coronal sections was obtained with matrix implanted
laser desorption/ionization mass spectrometry imaging (MILDI-MSI)
by implanting silver nanoparticulate matrices followed by focused
laser desorption. Most of the CER and SM quantified in whole brain
extracts were detected in MILDI images. Coronal sections from three
brain levels show qualitative regional changes in CER-SM ion intensities,
as a function of group and brain region, in cortex, striatum, accumbens,
habenula, and hippocampus. Highly correlated changes in certain white
matter CER-SM pairs occur in regions across all groups, including
the hippocampus and the lateral (but not medial) cerebellar cortex
of adult mice. Our data provide the first microscale MS evidence of
regional lipid intensity variations induced by alcohol