Additional file 4: Figure S3. of Urinary chitinase 3-like protein 1 for early diagnosis of acute kidney injury: a prospective cohort study in adult critically ill patients

Distribution of urinary chitinase 3-like protein 1 (UCHI3L1) (a) and urinary neutrophil gelatinase-associated lipocalin (UNGAL) (b) at enrollment in the eight selected subgroups of patients who did not develop acute kidney injury (AKI) based on the Kidney Disease | Improving Global Outcomes serum creatinine (SCr) or urine output (UO) criteria (No AKI SCr/UO ) within 7 days after enrollment, compared to the distribution in all patients with no AKISCr/UO within 12 h after enrollment, and in all those maximally reaching AKISCr/UO stages 1, 2, or 3 within 12 h after enrollment. (TIF 715 kb

Additional file 5: Figure S4. of Urinary chitinase 3-like protein 1 for early diagnosis of acute kidney injury: a prospective cohort study in adult critically ill patients

Distribution of urinary chitinase 3-like protein 1 (UCHI3L1) (a) and urinary neutrophil gelatinase-associated lipocalin (UNGAL) (b) at enrollment in the eight selected subgroups of patients who did not develop acute kidney injury (AKI) based on the Kidney Disease | Improving Global Outcomes serum creatinine (SCr) criteria (No AKI SCr ) within 7 days after enrollment, compared to the distribution in all patients with no AKISCr within 24 h after enrollment, and in all those maximally reaching AKISCr stages 1, 2, or 3 within 24 h after enrollment. (TIF 716 kb

Additional file 2: Figure S1. of Urinary chitinase 3-like protein 1 for early diagnosis of acute kidney injury: a prospective cohort study in adult critically ill patients

Area under the receiver-operating characteristics curve (AUC-ROC) with 95 % confidence interval (CI) of urinary chitinase 3-like protein 1 (UCHI3L1) (a) and urinary neutrophil gelatinase-associated lipocalin (UNGAL) (b) at enrollment for predicting acute kidney injury (AKI) stage ≥2 based on the Kidney Disease | Improving Global Outcomes (KDIGO) serum creatinine (SCr) or urine output (UO) criteria (AKI SCr/UO ) within 12 h in different subgroups of patients. (TIF 434 kb

Additional file 2: of Long-term quality of life in critically ill patients with acute kidney injury treated with renal replacement therapy: a matched cohort study

SF-36 assessments over time. In this additional file, evolutions in SF-36 assessments are described through figures in the 1-year cohort (47 AKI-RRT (A) and 94 non-AKI-RRT patients (B)) and in the 4-year cohort (28 AKI-RRT (C) patients and 28 non-AKI-RRT patients (D)). Percentages of patients with some or severe problems in the different domains of the SF-36 are given over the different time points: baseline, 3 months and 1 year (1-year cohort) and baseline, 3 months, 1 year and 4 years (4-year cohort). (PDF 126 kb

Additional file 1: Table S1. of Long-term outcome in ICU patients with acute kidney injury treated with renal replacement therapy: a prospective cohort study

Cox proportional hazards model. Table S2. Multivariate regression analysis: MAKE at 1 year. (DOCX 15 kb

Overview of the detection limit and the ITS2-fragment length of the reference strains.

<p>Legend: ATCC: American Type Culture Collection, Rockville, Maryland; IHEM: Institute for Hygiene & Epidemiology, Mycology, Brussels, Belgium; CS Fungal Biodiversity Centre; J: non-CBS strains, obtained from CBS.</p><p>Overview of the detection limit and the ITS2-fragment length of the reference strains.</p

A schematic overview of the study set up.

<p>A schematic overview of the study set up.</p

Detailed overview of the discrepancies, divided into following categories: Category A: direct method positive, but indirect method negative (n = 38); Category B: direct method more species than indirect method (n = 6); Category C: indirect method positive, but direct method negative (n = 2); Category D: indirect method more species than direct method (n = 9); and Category E: aspecific detection of nonfungal species by indirect method (n = 3).

<p>Legend: ALB: <i>Candida albicans</i>; DUB: <i>Candida dubliniensis</i>; GLA: <i>Candida glabrata</i>; GUI: <i>Candida guilliermondii</i>; LUS: <i>Candida lusitaniae</i>; PAR: <i>Candida parapsilosis</i>; ROB: <i>Candida robusta</i>; TRO: <i>Candida tropicalis</i>; ACHXYL: <i>Achromobacter xylosoxidans</i>; >: The left species is more abundant than the right species; m: minor discrepancy; M: Major discrepancy; N: No species detected, below detection level; S: Sample; D: Direct method; I: Indirect method; +: Indication of the quantity of a species, on the basis of number of colonies cultured;?: Unknown melting peak pattern.</p><p>^a: Number of samples for which the direct method established the presence of more species than the indirect method (total number: 44).</p><p>^b: Pattern of low intensity with unknown peak. Sequencing yielded an unreliable identification (closest match <i>Cladosporium cladosporioides</i>).</p><p>^c: Pattern of low intensity, similar to the pattern observed with a mixture of <i>C</i>. <i>glabrata</i> and <i>C</i>. <i>robusta</i>. Sequencing yielded an unreliable identification (closest match <i>Physarum loratum</i>).</p><p>^d: Unknown peak at a Tm of 88.78°C</p><p>Detailed overview of the discrepancies, divided into following categories: Category A: direct method positive, but indirect method negative (n = 38); Category B: direct method more species than indirect method (n = 6); Category C: indirect method positive, but direct method negative (n = 2); Category D: indirect method more species than direct method (n = 9); and Category E: aspecific detection of nonfungal species by indirect method (n = 3).</p

Graphic display of the ITS2-HRM curve of the sample containing <i>C</i>. <i>kefyr</i>, <i>C</i>. <i>parapsilosis</i> and <i>Pichia manshurica</i>, according to culture.

<p>ITS2-HRM patterns of strains of each species are added for comparison.</p

Evaluation of the impact on the shape of the melting peak pattern by analyzing 5 different experimental ratios between <i>Candida albicans</i> and <i>C</i>. <i>glabrata</i>, in which the latter one is respectively 250 (a), 200 (b), 100 (c), 10 (d) and 4 (e) fold more present.

<p>Evaluation of the impact on the shape of the melting peak pattern by analyzing 5 different experimental ratios between <i>Candida albicans</i> and <i>C</i>. <i>glabrata</i>, in which the latter one is respectively 250 (a), 200 (b), 100 (c), 10 (d) and 4 (e) fold more present.</p