Additional file 15: Table S10. of Developmental piRNA profiles of the invasive vector mosquito Aedes albopictus

Sex-bias piRNA loci in the genome. (XLS 40 kb

Additional file 12: Figure S5. of Developmental piRNA profiles of the invasive vector mosquito Aedes albopictus

Characterization of gene and repetitive elements-derived piRNAs. a Ratios of piRNAs with uridine at their 5′ ends (upper panel) and piRNAs with adenosine at position 10 (lower panel) in various developmental stage libraries. b Ratios and abundances (lower panel) of in various developmental stage libraries. b Strand bias in various developmental stage libraries. Abundance and percentage of CDSs, 5′ UTR- and 3′ UTR-derived piRNAs. c Ping-pong pair analysis of gene and repetitive elements-derived piRNAs. The length of overlap is shown on the horizontal axes. Indicated above each axis is the number of possible overlapping pairs of small RNAs with a specified overlap size. Indicated below each axis is the relative frequency of the 5′ base identity for overlapping sequences. The colour code for bases is indicated in the centre box. (TIF 4512 kb

Additional file 2: Table S2. of Developmental piRNA profiles of the invasive vector mosquito Aedes albopictus

Small RNA sequence statistics for 24–30 nt reads matched to the Aedes albopictus genome assembly. (XLS 32 kb

Additional file 1: Table S1. of Developmental piRNA profiles of the invasive vector mosquito Aedes albopictus

Small RNA sequence statistics for the six developmental stage libraries of Aedes albopictus. (XLS 33 kb

Additional file 11: Table S7. of Developmental piRNA profiles of the invasive vector mosquito Aedes albopictus

The top 10 abundance of transcription of piRNAs clusters from each developmental library. (XLS 39 kb

Binding affinities of AalbOBP37 and AalbOBP39.

<p>At pH 7.4, AalbOBP37 (A) and AalbOBP39 (B) bind four selected ligands, and their affinities to indole were higher at pH 7.4 than those at pH 4.5 (C&D), which showed the binding affinities of AalbOBP37 and AalbOBP39 were affected by different pH condition. Methanol was used as solvent and showed the lowest binding affinity to both OBPs. Error bars show standard deviation.</p

EAG responses of female <i>Ae. albopictus</i> antennae.

<p>(A) EAG response recording from female antennae showed a dose-dependent manner. Doses of selected ligands: 25 ug (green), 50 ug (purple) and 100 ug (blue), n = 6–8; mean ± std. (B) Doses of four compounds, 100 ug; n = 6–8; mean ± std, mosquito age: 4–5 days old. EAG response of 1-octen-3-ol was used as a standard (100%) to normalize those elicited by other chemicals. Indole and skatole elicited more than 50% of the 1-octen-3-ol response. (C) EAG response post-RNAi-treatment. Compound: indole, 100 ug, n = 6–8; mean±std; mosquito: 4–5 day post RNAi treatment. Detection was separated into non-injected group, dsRNA-dsRED injected group, dsRNA-OBP37 injected group and dsRNA-OBP39 injected group. Hexane (10 ul) was used as a control in this study.</p

Hierarchical clustering of miRNA expression.

<p>Normalized expression profiles of 215 miRNA/miRNA*s from six different developmental stages were clustered. Stages are in columns and miRNAs in rows. Red indicates that a gene is represented highly at the stage, whereas green indicates the opposite. miRNAs with similar expression patterns cluster together. There are five Clusters (1–5) with variable numbers of sub-clusters. Abbreviations: <b>E</b>, embryos; <b>L</b>, larvae; <b>P</b>, pupae; <b>M</b>, adult males; <b>F</b>, adult females and <b>B</b>, blood-fed adult females.</p

The associations between <i>NFKB1</i> promoter –94 ins/del ATTG polymorphism and the development of bladder cancer.

<p>Abbreviations: CI, confidence interval; OR, odds ratio.</p>a<p>Two-sided χ² test for either genotype distributions or allele frequencies between the cases and controls.</p>b<p>Adjusted for age, gender, smoking, drinking status and family history of cancer in logistic regression model.</p

<i>Aedes albopictus</i> small RNA sequences match known <i>Aedes aegypti</i> pre-miRNAs.

<p>Two examples are shown for <b><i>miR-375</i></b> (<b>A</b>) and <i>miR-92b</i> (<b>B</b>). The portions of the pre-miRNA sequences (<b><i>aae-miR-375 pre-miRNA</i></b><b> and </b><b><i>aae-miR-92b pre-miRNA</i></b>) from <i>Ae. aegypti</i> with similarity to the <i>Ae. albopictus</i> reads are shown at the bottom of each image. Nucleotides in blue are those that contribute to the secondary structure of the pre-miRNA hairpin. Abbreviations: <b>nt</b>, length of small RNA read in nucleotides; <b>S</b>, sample from which the small RNAs were sequenced; <b>M</b>, adult male; <b>F</b>, adult female; <b>L</b>, larvae; <b>E</b>, embryos; <b>B</b>, blood-fed female; <b>N</b>, number of reads in each sample that showed the exact sequence; %, percentage of sequence identity between the <i>Ae. albopictus</i> small RNA read and the <i>Ae. aegypti</i> pre-miRNA. Nucleotides in red indicate sequence differences between <i>Ae. albopictus</i> and <i>Ae. aegypti</i> in the miRNA*. The 5′- to 3′-orientation of the sequences is listed from left to right in the image.</p