Study subject characteristics.

<p>Study subject characteristics.</p

BKPyV specific (A) CD4⁺ and (B) CD8⁺ T-cells.

<p>Line represents mean value. “-” = BK Negative, “U” = viruria, “P” = viremia, “mo” = months post-transplant. “Pre-Tx” = Pre-transplant. P values were calculated by Kruskall-Wallis comparing all 3 groups at each time point. <b>A</b>: No significant difference at any time point. <b>B</b>: 3 month mean %IFN-γ producing CD8⁺ T-Cells for BK negative 1.29 (±2.36), viruria 1.54 (±1.21), viremia 6.67 ±0.54), P = 0.02; 6 months mean for BK negative 1.81 (±4.10), viruria 12.65 (±11.10) and viremia 17.22 (±18.68), P = 0.05; 12 months mean for BK negative 0.53 (±0.91), viruria 9.44 (±7.13), viremia 10.97 (±8.85), P = 0.01.</p

Phenotypic composition of T lymphocytes in the BK negative, viruria, and viremia groups.

<p><b>2A: CD4+ T-Cell Phenotypes</b> *P < 0.05. “Pre-Tx” = Pre-transplant. “mo” = Months post-transplant. P values were calculated by Kruskall-Wallis comparing each T-cell subgroup at each time point. Effector Cells are CD45RO-/CD27-; Effector Memory Cells are CD45RO+/CD27-; Central Memory Cells are CD45RO+/CD27+; Naïve Cells are CD45RO-/CD27+. Expressed are means as a percent of total CD4+ T-Cells, may not add to 100% due to rounding. *Effector memory cells at 3 months in BK negative 15.93% (±11.29), viruria 20.00% (±12.00) and viremia 5.59% (±2.32) P = 0.05. <b>2B: CD8+ T-Cell Phenotypes</b> *P < 0.05. “Pre-Tx” = Pre-transplant. “mo” = Months post-transplant. P values were calculated by Kruskall-Wallis comparing each T-cell subgroup at each time point. Effector Cells are CD45RO-/CD27-; Effector Memory Cells are CD45RO+/CD27-; Central Memory Cells are CD45RO+/CD27+; Naïve Cells are CD45RO-/CD27+. Expressed are means as a percent of total CD8+ T-Cells, may not add to 100% due to rounding. *Baseline percent effector cells for viremia is 21.41% (±13.11), viruria 4.04% (±4.51) and BK negative is 5.49% (±4.76), P = 0.04. 12 month percent effector cells for viremia is 14.49% (±3.76), viruria 3.86% (±1.64), and BK negative 4.10% (±4.19), P = 0.01.</p

Univariable GEE logistic regression for BKPyV viremia.

<p>Univariable GEE logistic regression for BKPyV viremia.</p

Anti-BKPyV IgG.

<p>Line represents mean value. “OD” = Optical Density, “-” = BK Negative, “U” = viruria, “P” = viremia, “mo” = months post-transplant. “Pre-Tx” = Pre-transplant. P values were calculated by Kruskall-Wallis comparing all 3 groups at each time point. 12 month mean for BK negative 1.25 (± 0.70), viruria 2.03 (± 0.43), viremia 2.15(± 0.02), P = 0.02.</p

GEE logistic regression for BKPyV reactivation.

<p>GEE logistic regression for BKPyV reactivation.</p

GEE Linear regression for change in variable per month post-transplant.

<p>GEE Linear regression for change in variable per month post-transplant.</p

Prevalence of BKPyV detected in the plasma or urine.

<p>Prevalence of BKPyV detected in the plasma or urine.</p