DEVELOPMENT & VALIDATION OF HPLC METHOD FOR ANALYSIS OF SOME ANTIHYPERTENSIVE AGENTS IN THEIR PHARMACEUTICAL DOSAGE FORMS

A simple, fast, specific and accurate reverse-phase high performance liquid chromatography (RP-HPLC) method has been developed and subsequently validated for simultaneous estimation of Aliskiren (ALN) and Felodipine (FLD) from their dosage forms. Agilent 1200 infinity LC equipped with UV-Visible with EZChrom Elite compact version 3.3.2 SP2 software was used. Chromatographic separation was achieved isocratically on a Hypersil BDS C8 column (150×4.6 mm, 5 μ particle size) using a mobile phase, Water (adjusted to pH 3.0 with 10%v/v ortho-phosphoric acid) , Acetonitrile and Methanol in the ratio of  20:30:50 v/v/v and UV detection was carried out at 254 nm for both the drugs ALN and FLD. The retention time for ALS and FLD was found to be 4.26 and 2.96 min respectively, and recoveries were found between 98 and 102%.  The method was found linear over the range of 150-450 µg mL-1 for ALS and  5-15 µg mL-1 for FLD. The method has been successfully applied to assess the assay of solid dosage formulations

Imaging of Pediatric Benign Bone Tumors

Benign bone tumors in the pediatric population can have varied clinical presentations ranging from asymptomatic to nonspecific pain, swelling, or pathological fracture. A systemic imaging approach should be utilized to evaluate for focal bone abnormalities. Radiologists must be aware of salient imaging features of pediatric benign bone tumors, as it helps to guide clinicians for further management and help decreasing patient anxiety and unnecessary medical intervention

Role of fetal monitoring in high risk pregnancy by fetal electrocardiogram

Background: Non-stress test is an external monitoring of fetal heart rate by electrocardiograph. Although intermittent auscultation of fetal heart rate is equivalent to continuous electronic fetal monitoring in detecting fetal compromise1 but continuous electronic fetal monitoring is indicated in high risk patients women whose foetuses are at high risk for neonatal encephalopathy or cerebral palsy.2 Objective         of current study was to study the efficacy and diagnostic value of non-stress Test for surveillance and its usefulness to detect fetal distress at early stage which help to decide further management in mode of delivery.Methods: Design: prospective study. NST was done in 50 high risk patients for minimum of 20 minutes and in patients with non-reactive non stress test it was continued for 40 minutes. Maternal age, parity, complications during labour, and delivery, mode of delivery, indications of caesarean section and perinatal outcome were noted. Results: Out of total 50 cases studied patient delivered vaginally were 24 and Caesarean was done in 26 cases. Most LSCS were performed due to PIH (35%) and related complications like IUGR, eclampsia (10%), fetal distress, previous caesarean pregnancy, IUGR, oligohydraminos and meconium stained liquor. 52% patients were delivered by caesarean and 48% by normal delivery.Conclusions: Routine use of electronic fetal heart monitoring helped in reduction of neonatal morbidity and mortality with increased rate of caesarean section

ELECTROPHYSIOLOGICAL CHANGES IN LATENCY IN DIABETIC RETINOPATHY: AN OBSERVATIONAL STUDY

Purpose: Multifocal ERG is a useful indicator of diabetic retinopathy. The significant delay in local responses provides a chance for the detection and understanding of the various stages of diabetic retinopathy. Materials and Methods: This is a cross sectional study conducted in ERG clinic at M & J Western regional institute of ophthalmology, Ahmedabad from January 2013 to September 2015 who were more than 35yrs of age. Results: In our study, we studied 45 eyes of diabetic patients and 20 eyes of normal subjects. In our study the mean values of the various parameters was calculated in the control group with N1, P1 and N2 latency being 14.09ms. 29,76ms and 45.55ms respectively. The N1, P1 and N2 amplitude was found to be 31.52nV, 73.61nV and 90.38nV respectively. The maximum delay in N1, P1 and N2 latency was seen to be 3.24ms, 7.11ms and 8.40ms respectively from the normal value. We also found a decrease in amplitude of the ERG waveform with N1, P1 and N2 amplitude being 20.98nV, 61.48nV and 76.4nV respectively from the normal value. Also it is helpful in cases with clinically significant macular edema where responses are remarkably delayed suggesting local retinal dysfunction and macular pathology. It provides us information regarding the condition of the macula and some ideas about the extent of ischemia affecting this area. Conclusion: In conclusion, we can say that the delayed responses obtained indicate abnormal retinal function corresponding to local discrete retinal lesions. It provides a very sensitive and objective assessment of the local retinal condition in various stages of diabetic retinopathy. KEYWORDS: Diabetes mellitus; Diabetic retinopathy; Multifocal electroretinogram

ELECTROPHYSIOLOGICAL CHANGES IN LATENCY IN DIABETIC RETINOPATHY: AN OBSERVATIONAL STUDY

Purpose: Multifocal ERG is a useful indicator of diabetic retinopathy. The significant delay in local responses provides a chance for the detection and understanding of the various stages of diabetic retinopathy. Materials and Methods: This is a cross sectional study conducted in ERG clinic at M & J Western regional institute of ophthalmology, Ahmedabad from January 2013 to September 2015 who were more than 35yrs of age. Results: In our study, we studied 45 eyes of diabetic patients and 20 eyes of normal subjects. In our study the mean values of the various parameters was calculated in the control group with N1, P1 and N2 latency being 14.09ms. 29,76ms and 45.55ms respectively. The N1, P1 and N2 amplitude was found to be 31.52nV, 73.61nV and 90.38nV respectively. The maximum delay in N1, P1 and N2 latency was seen to be 3.24ms, 7.11ms and 8.40ms respectively from the normal value. We also found a decrease in amplitude of the ERG waveform with N1, P1 and N2 amplitude being 20.98nV, 61.48nV and 76.4nV respectively from the normal value. Also it is helpful in cases with clinically significant macular edema where responses are remarkably delayed suggesting local retinal dysfunction and macular pathology. It provides us information regarding the condition of the macula and some ideas about the extent of ischemia affecting this area. Conclusion: In conclusion, we can say that the delayed responses obtained indicate abnormal retinal function corresponding to local discrete retinal lesions. It provides a very sensitive and objective assessment of the local retinal condition in various stages of diabetic retinopathy. KEYWORDS: Diabetes mellitus; Diabetic retinopathy; Multifocal electroretinogram

Review of code blue system and audit

Background: Code Blue systems are communication systems that ensure the most rapid and effective resuscitation of a patient in respiratory or cardiac arrest. Code blue was established in Bharati Hospital and Research Centre in Sept 2011 in order to reduce morbidity and mortality in wards. The aim of the study was to evaluate the current code blue system and suggest possible interventions to strengthen the system.Methods: It was retrospective observational descriptive study. The study population included all consecutive patients above the age of 18 years for whom code blue had been activated. Data was collected using code blue audit forms. The data was analysed using SPSS (Statistical Package for social sciences) software.Results: A total of 260 calls were made using the blue code system between September 2011 to December 2012. The most common place for blue code activation was casualty. The wards were next, followed by dialysis unit and OPD. The indications for code blue team activation were cardio-respiratory arrest (CRA) (88 patients, 33.84%), change in mental status (52 patients, 20%), road traffic accidents RTA (21, 8.07%), convulsions (29 patients 11.15%), chest pain (19 patients, 8.46%), breathlessness (18 patients,6.92%) and worry of staff about the patient (17 patients, 6.53%), presyncope (10 patients, 3.84%), and others (6 patients, 2.30%). The average response time was 1.58±0.96 minutes in our study. Survival rate was more in medical emergency group 46.15% than in CRA group 31.61%. Initial success rate was 35.2% and a final success rate was 34.6%.Conclusions: Establishment of code blue team in the hospital enabled us to provide timely resuscitation for patients who had “out of ICU” CRA. Further study is needed to establish the overall effectiveness and the optimal implementation of code blue teams. The increasing use of an existing service to review patients meeting blue code criteria requires repeated education and a periodic assessment of site-specific obstacles to utilization

Review on Synchronization for OFDM Systems

Orthogonal Frequency Division Multiplexing (OFDM) is a multi-carrier modulation scheme. It is widely used modulation technique because it has high data rate, high spectral efficiency and robustness to multipath fading channel. One of the major drawbacks of OFDM system is synchronization. It is very sensitive to frequency synchronization errors in the form of Carrier Frequency Offset (CFO). The Carrier Frequency Offset can causes Inter Carrier Interference (ICI) and destroy the orthogonality of the OFDM system. Therefore it is necessary to perform frequency synchronization. In this paper various Carrier Frequency Offset Estimation methods are presented

ON PILOT CONTAMINATION IN MASSIVE MULTIPLE-INPUT MULTIPLE OUTPUT SYSTEM WITH LEAST SQUARE METHOD AND ZERO FORCING RECEIVER

Massive Multiple-Input Multiple-Output (MIMO) wireless communications refers to use of large number of antennas at transmitter and receiver to enhance spectral and energy efficiency. However, its performance is limited by a problem known as pilot contamination. In this paper, we present a comprehensive overview of massive MIMO system and studied degradation in performance due to pilot contamination. To showcase such effects, we have implemented the channel estimation using Least Square (LS) method with random pilots and time shifted pilot scheme through simulations. In this study we have used zero forcing (ZF) receivers. We have also studied performance improvement in presence of pilot using MMSE receiver. Further improvement is achieved in this work by introducing precoding technique for massive MIMO systems