Additional file 3: Table S3. of Transcriptome analysis reveals potential mechanisms underlying differential heart development in fast- and slow-growing broilers under heat stress

Significant DE genes (FDR < 0.1, |log2FC| < 1) from EdgeR for comparisons between heat stress and thermoneutral groups in Ross 708 and Illinois broilers (XLSX 38 kb

Additional file 1: Table S1. of Transcriptome analysis reveals potential mechanisms underlying differential heart development in fast- and slow-growing broilers under heat stress

Primers used in Biomark 48.48 IFC for the validations of RNA-seq data (PDF 110 kb

Additional file 2: Table S2. of Transcriptome analysis reveals potential mechanisms underlying differential heart development in fast- and slow-growing broilers under heat stress

Statistical summary of sequence reading, mapping and counting (PDF 103 kb

Identification of CTLA2A, DEFB29, WFDC15B, SERPINA1F and MUP19 as Novel Tissue-Specific Secretory Factors in Mouse

<div><p>Secretory factors in animals play an important role in communication between different cells, tissues and organs. Especially, the secretory factors with specific expression in one tissue may reflect important functions and unique status of that tissue in an organism. In this study, we identified potential tissue-specific secretory factors in the fat, muscle, heart, lung, kidney and liver in the mouse by analyzing microarray data from NCBI’s Gene Expression Omnibus (GEO) public repository and searching and predicting their subcellular location in GeneCards and WoLF PSORT, and then confirmed tissue-specific expression of the genes using semi-quantitative PCR reactions. With this approach, we confirmed 11 lung, 7 liver, 2 heart, 1 heart and muscle, 7 kidney and 2 adipose and liver-specific secretory factors. Among these genes, 1 lung-specific gene - CTLA2A (cytotoxic T lymphocyte-associated protein 2 alpha), 3 kidney-specific genes - SERPINA1F (serpin peptidase inhibitor, Clade A, member 1F), WFDC15B (WAP four-disulfide core domain 15B) and DEFB29 (defensin beta 29) and 1 liver-specific gene - MUP19 (major urinary protein 19) have not been reported as secretory factors. These genes were tagged with hemagglutinin at the 3’end and then transiently transfected to HEK293 cells. Through protein detection in cell lysate and media using Western blotting, we verified secretion of the 5 genes and predicted the potential pathways in which they may participate in the specific tissue through data analysis of GEO profiles. In addition, alternative splicing was detected in transcripts of CTLA2A and SERPINA1F and the corresponding proteins were found not to be secreted in cell culture media. Identification of novel secretory factors through the current study provides a new platform to explore novel secretory factors and a general direction for further study of these genes in the future.</p></div

Conversion process from SRF into biodiesel via BPL.

<p>Conversion process from SRF into biodiesel via BPL.</p

Crude oil content in BPL biomass grown on solid organic wastes.

<p>Crude oil content in BPL biomass grown on solid organic wastes.</p

Properties of <i>B. peregrine</i> larvae oil and three known oils.

<p>n/a stands for not reported;</p>a<p>data from literature <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0045940#pone.0045940-Zheng1" target="_blank">[10]</a>;</p>b<p>data from literature <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0045940#pone.0045940-Li3" target="_blank">[11]</a>;</p>c<p>data from literature <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0045940#pone.0045940-Demirbas1" target="_blank">[20]</a>.</p

Properties of <i>B. peregrine</i> larvae oil-based biodiesel and three known biodiesels.

<p>n/a stands for not reported;</p>a<p>data from literature <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0045940#pone.0045940-Zheng1" target="_blank">[10]</a>;</p>b<p>data from literature <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0045940#pone.0045940-Li3" target="_blank">[11]</a>;</p>c<p>data from literature <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0045940#pone.0045940-Demirbas1" target="_blank">[20]</a>.</p

Gene expression values of selected genes.

<p>^a-e Different superscripts within a row indicate significant difference (P<0.05).</p><p>The highest expression value for each gene is highlighted in bold font. A/O: ratio between average expression of certain gene in tissue with specific expression of that gene and expression in other tissues. EM: Extracellular matrix; ER: Endoplasmic reticulum.</p><p>Gene expression values of selected genes.</p

Optimization of four esterification parameters.

<p>A) methanol to oil molar ratio (1.0% catalyst, 65°C, 60 min, 200 rpm); B) catalyst amount (12∶1 methanol to oil molar ratio, 65°C, 60 min, 200 rpm); C) temperature (1.5% catalyst, 12∶1methanol to oil molar ratio, 60 min, 200 rpm); D) reaction time (1.5% catalyst, 12∶1methanol to oil molar ratio, 70°C, 200 rpm). Each experiment was repeated for three times.</p