Additional file 1: Figure S1. of Genome-wide analysis of a avirulent and reveal the strain induces pro-tective immunity against challenge with virulent Streptococcus suis Serotype 2

Comparing of the MRPs from four strains of S.suis 2. (A) Alignment of the amino acids sequences of MRP. (B) Phylogenetic comparison of MRP proteins. Figure S2 A novel system responsible for L-fucose metabolism from the GEI of 48 k. (A) L-fucose metabolic gene cluster. (B) Illustration of L-fucose metabolic pathway. Table S1 Prediction of antigenic peptides of MRPs from four strains of S.suis 2. (DOCX 552 kb

Microscopic Characterization of Sectioned Liver Tissue from Patients Who Had Died

<div><p>(A) Light image of a liver tissue section (×100). The central vein is indicated with an arrow.</p> <p>(B) Light image of a liver tissue section (×200).</p> <p>(C) The convergent zone is indicated with an arrow (×100).</p> <p>(D) TEM image of a liver tissue section (×20,000). A bacterium found in the tissue is highlighted with an arrow.</p></div

Phylogenetic Trees of Six Representative Isolates Based on Comparison of 16S rDNA and Five Putative Virulence-Associated-Factor Genes with Known Sequences

<p>Swine isolates from Sichuan ( S. suis ZYS3 and S. suis ZYS8) labeled in green, human isolates ( S. suis ZYH13 and S. suis ZYH14) from Sichuan labeled in red, Jiangsu isolates from 1998 ( S. suis 9801 and S. suis Habb) labeled in blue, and the standard highly virulent strain S. suis P1/7 labeled in pink. All representative strains from other streptococcus species or isolates of S. suis 2 are as indicated in the tree. </p

RFLP Analysis of Different S. suis 2 Isolates

<p> S. suis S10: a highly virulent strain from China; <i>S</i>. <i>suis</i> 9801: swine isolate from Jiangsu Province in 1998; S. suis Habb: human isolate from Jiangsu Province in 1998; S. suis ZYS3: swine isolate from Sichuan Province in 2005; S. suis ZYH13: human isolate from Sichuan Province in 2005; M: 1 kb DNA Ladder (MBI Ferments, Gdansk, Poland). </p

Immunohistochemical Analysis of Liver Tissue Sections from Dead Patients Incubated with Normal and S. suis 2-Infected Swine Serum

<div><p>(A) Staining with normal swine serum detected no S. suis 2 antigen. </p> <p>(B) Staining with the serum from the infected swine indicated the presence of S. suis 2 antigen. </p></div

Detection of the Pathogenic SS2 and Identification of Its Specific Genes

<div><p>(A) Light microscopy image of the isolates cultured from autopsy specimens.</p> <p>GP⁺ cocci (pointed to with black arrows) are arranged in various short chains (×100). </p> <p>(B) Qualitative PCR detection of isolates from the liver of fatal human cases with a set of primers specific for <i>S</i>. <i>suis</i> 2. M: 100bp DNA marker (Fermentas, Vilnius, Lithuania). CK: 16S rDNA PCR product from the R 735 standard strain of S. suis 2. Multi-PCR: performed with a set of unique primers specific for <i>mrp, epf, suilysin,</i> and <i>cps-2J,</i> respectively. </p></div

Statistics of PCR detection for 89K in SS2

*<p>the genomes of Chinese SS2 strains have been sequenced.</p>#<p>those strains were collected in Academy of Military Medical Sciences, P. R. China.</p>+<p>being positive in PCR assay for 89K.</p>−<p>being negative in PCR assay for 89K.</p

Genome-wide display of virulence associated factors/pathways in SS2

–<p> <b>not found</b></p

Figure 5

<p>RT-PCR analysis of 89K expression profile exemplified by a TCS. (A) RT-PCR analysis of the response regulator (05SSU0943) at its transcriptional level. The PCR product (05SSU0943) at the position of the arrow (∼0.6 kb), is used as control (CK). (B) RT-PCR analysis of the sensor histidine kinase (05SSU0944) at its transcriptional level. The PCR product (05SSU0944) functions as control (CK) at the position of the arrow (∼0.2 kb). (C) RT-PCR analysis of 16S rRNA, one of the most housekeeping genes featuring the constitutive expressions. The expected fragment is of ∼1.3 kb at the arrow position. (D) Electrophoresis analysis of the total RNA isolated from both representative SS2 strains (98HAH12&05ZYH33). Two conservative subunits of bacterial rRNAs are pointed out with 16S and 23S, respectively.</p