19 research outputs found
Additional file 1: Table S1. of WT1-AS promotes cell apoptosis in hepatocellular carcinoma through down-regulating of WT1
Sequence information for primer and siRNA. (DOCX 13Â kb
The immunohistochemistry analysis of epithelial cell kinase (EphA2).
<p>Control group (a), EphA2 protein levels from the 1st day to the 1st week (b, c, d, e) (P<0.01), and from the 2nd week to the 4th week (a vs h P<0.05). Original magnification, ×200. Scale bars: 20 µm.</p
Hypoxia inducible factor-1, 2α, VEGFA, and EphA2 mRNA expression measured by Q-PCR.
<p>HIF-1α mRNA expression at the 3<sup>rd</sup> day (P<0.01), compared with the control, and other groups of the time points (P<0.05). HIF-2α mRNA at the 2<sup>nd</sup> week (P<0.01) and levels of VEGFA and EphA2 mRNA at various time points from the 1st day to the 1st week, P<0.05, and from the 3 w to 4 w, P<0.05, compared with control group, and the 2nd week vs the control group, P>0.05.</p
The VEGFA and EphA2 protein expression after HIFU treatment from 1 d to 1 w, compared with the control group (P<0.01) and from the 3 w to 4 w compared to the control group, respectively (P<0.05).
<p>(2nd week vs control group, P>0.05).</p
MVD analyses.
<p>Control group (a), micro-vessel density (MVD) from the 1<sup>st</sup> day to the 1<sup>st</sup> week (b, c, d, e) (P<0.01), and from the 2nd week to the 4th week (g vs a, P<0.05). Original magnification, ×200. Scale bars:20 µm.</p
HIF-1α protein expression on the 3<sup>rd</sup> day, compared with the control group and other groups of the time points (P<0.01).
<p>(1<sup>st</sup> day vs the 1<sup>st</sup> week, P>0.05, from the 2 w to 4 w group, compared with each other, P>0.05).</p
The HIF-2α protein levels on the 2<sup>nd</sup> week, compared with the control group (P<0.01), from the 2<sup>nd</sup> to 4<sup>th</sup> week (P<0.05, compared with the control group).
<p>The HIF-2α protein levels on the 2<sup>nd</sup> week, compared with the control group (P<0.01), from the 2<sup>nd</sup> to 4<sup>th</sup> week (P<0.05, compared with the control group).</p
An immunohistochemistry analysis of hypoxia inducible factor-2α (HIF-2α).
<p>Control group (a), HIF-2α protein levels on the 2<sup>nd</sup> week (f vs a, b, c) (P<0.01), and the 2<sup>nd</sup> to 4<sup>th</sup> week (P<0.05). Original magnification, ×200. Scale bars: 20 µm.</p
An immunohistochemistry analysis of vascular endothelial growth factor A (VEGF-A).
<p>Control group (a), HIFU treatment from the 1st day to the 1st week (b, c, d, e) (P<0.01), and from the 2st week to the 4st week (a vs h, P<0.05). Original magnification, ×200. Scale bars: 20 µm.</p
The picture shows residual hepatic tumor tissue after high intensity focused ultrasound (HIFU) treatment.
<p>(<b>a</b>) The residual hepatic tumor tissue was obtained from the subcutaneously transplanted tumor. (<b>b</b>) The cut surface of the residual tumor tissue. (<b>c</b>) Residual tumor tissue with hematoxylin and eosin staining, revealing necrotic cells (<b>d</b>), inflammatory cells (<b>e</b>), and normal hepatoma cells (<b>f</b>). Original magnification, ×200. Scale bars: 20 µm.</p