Prevalence of <i>F. nucleatum</i> and <i>fadA</i> among four groups.

<p>P<0.05 between OG/NOG/PG and CG; **P<0.01 between OG/NOG/PG and CG (Chi-squared test).</p

Detection and distribution of <i>F. nucleatum/fadA</i>.

<p>a. Detection of <i>F. nucleatum</i> in clinical subgingival biofilm samples. M:Marker;lane 1:positive control of <i>F. nucleatum</i> ATCC25586;lane 2:blank; lane 3∼10:positive clinical samples;lane 11 and12:negative clinical samples; b. Detection of <i>fadA</i> in clinical subgingival biofilm samples. M:Marker;lane 1:positive control of <i>F. nucleatum</i> ATCC25586;lane 2:blank;lane 3,4,6∼10:positive clinical samples;lane 5: negative clinical sample; c. Distribution of <i>F. nucleatum</i> in four groups. d. Distribution of <i>fad</i>A in four groups. ** P<0.01 between GI 2 and GI 0 in PG and NOG (c, d) (Chi-squared test).</p

Geography of Australian Mapputta group viruses.

<p>MAPV, Mapputta virus; MPKV, Maprik virus; TRUV, Trubanaman virus; GGV, Gan Gan virus; MURBV, Murrumbidgee virus; SASHV, Salt Ash virus; BUCV, Buffalo Creek virus.</p

Phylogenetic relationship of Australian Mapputta group and K10441 isolates to other selected orthobunyaviruses.

<p>Deduced amino acid sequences of the S- (N ORF; panel <b>A</b>), M- (Gn, NSm,Gc polyprotein ORF; panel <b>B</b>), and L-segment (RdRp-ORF; panel <b>C</b>) were aligned and trees reconstructed with the Neighbor-Joining method applying a Poisson substitution model as implemented in MEGA 6. Bootstrap values resulting from 1000 pseudoreplicates are indicated at the respective nodes; scale bars indicate the number of substitutions per site, and GenBank accession number and isolate name (where known) are given next to the virus name. MAPV, Mapputta virus; MPKV, Maprik virus; TRUV, Trubanaman virus; GGV, Gan Gan virus.</p

Predicted signalase cleavage sites.

<p>Predicted signalase cleavage sites.</p

Glycosylation sites of Australian orthobunyavirus M-segment polyprotein sequences.

<p>Predicted N-glycosylation sites are indicated along the M-segment sequence by their recognition sequence and aa position. MAPV, Mapputta virus; TRUV, Trubanaman virus; BUCV, Buffalo Creek virus; MURBV, Murrumbidgee virus; GGV, Gan Gan virus; SASHV, Salt Ash virus; MPKV, Maprik virus.</p

Characterized Australian orthobunyaviruses.

<p>Characterized Australian orthobunyaviruses.</p

Japanese encephalitis virus detections in Australia.

(DOCX)</p

Positively selected sites within the JEV/Australia/NT_Tiwi Islands/2021 genome.

(DOCX)</p

Maximum likelihood phylogenetic tree of JEV envelope genes available in GenBank.

The TIM2 model with gamma rate heterogeneity was chosen as the most appropriate model by IQ-TREE v.2.0.6. The results from 1000 bootstrap replicates are given on the nodes and the scale represents the number of nucleotide substitutions per site. (TIF)</p