Comparative genomics profiling of clinical isolates of using DNA microarrays-4

<p><b>Copyright information:</b></p><p>Taken from "Comparative genomics profiling of clinical isolates of using DNA microarrays"</p><p>BMC Genomics 2006;7():43-43.</p><p>Published online 7 Mar 2006</p><p>PMCID:PMC1434746.</p><p>Copyright © 2006 Nash et al; licensee BioMed Central Ltd.</p>n size. The amplicons were "binned" in 50 bp increments (to 1900 bp) then in 200 bp increments (from 2000 to 3000 bp). There were only 4 amplicons of size over 3000 bp

Comparative genomics profiling of clinical isolates of using DNA microarrays-2

<p><b>Copyright information:</b></p><p>Taken from "Comparative genomics profiling of clinical isolates of using DNA microarrays"</p><p>BMC Genomics 2006;7():43-43.</p><p>Published online 7 Mar 2006</p><p>PMCID:PMC1434746.</p><p>Copyright © 2006 Nash et al; licensee BioMed Central Ltd.</p>rol strain (higher copy number than in strain A449). Green indicates genes with lower signal intensity for the tester than the control (divergent in sequence or missing or at lower copy number). Gene order reflects results of hierarchical clustering of genes performed as described in Materials and Methods. The strains are ordered as in Figure 1, and unless otherwise noted, all other isolates are subsp. . Asm – subsp. , Asa – subsp. , Ass – subsp. . (A) All genes from the plasmid functional category. The blue bars (both light and dark) indicate genes found on plasmid 5, and the dark blue bars correspond to predicted TTSS genes. (B) All genes from the transposon functional category. The orange bars indicate genes with strong sequence similarity to known transposases

Comparative genomics profiling of clinical isolates of using DNA microarrays-1

<p><b>Copyright information:</b></p><p>Taken from "Comparative genomics profiling of clinical isolates of using DNA microarrays"</p><p>BMC Genomics 2006;7():43-43.</p><p>Published online 7 Mar 2006</p><p>PMCID:PMC1434746.</p><p>Copyright © 2006 Nash et al; licensee BioMed Central Ltd.</p>indicates genes with higher signal intensity for the tester than the control strain (higher copy number than strain A449). Green indicates genes with lower signal intensity for the tester than the control (divergent in sequence or missing or lower copy number). Gene order reflects results of hierarchical clustering of genes performed as described in Materials and Methods. The strains are ordered as in Figure 1, and unless otherwise noted, all other isolates are subsp. . Asm – subsp. , Asa – subsp. , Ass – subsp. . (A) All genes which are divergent in at least one of the sixteen isolates. Blue bars indicate genes associated with the plasmid category and orange bars indicate genes associated with the transposon category as described in Material and Methods. (B) Subset of genes which are divergent (Log Ratio< -1) in the fourteen strains and were not assigned to the plasmid or transposon functional categories. Genes predicted to code for OMPs (purple) and for flagella/pili proteins (grey) are indicated

Comparative genomics profiling of clinical isolates of using DNA microarrays-0

<p><b>Copyright information:</b></p><p>Taken from "Comparative genomics profiling of clinical isolates of using DNA microarrays"</p><p>BMC Genomics 2006;7():43-43.</p><p>Published online 7 Mar 2006</p><p>PMCID:PMC1434746.</p><p>Copyright © 2006 Nash et al; licensee BioMed Central Ltd.</p>istance and average linkage clustering (n = 1,000 bootstrap iterations). Isolates in bold are atypical isolates that cluster with other known subspecies. The bootstrap values which lead to their cluster assignment are also in bold. All ATCC type strains are denoted "ATCC", and unless otherwise noted, all other isolates are subsp. . (A) Sample clustering based on all genes on the AsalChip1 microarray. (B) Sample clustering based on genes not assigned to the plasmid or transposon functional categories (i.e. "chromosomal" genes)