Characterization of volatile compounds of Daucus crinitus Desf. Headspace Solid Phase Microextraction as alternative technique to Hydrodistillation

<p>Abstract</p> <p>Background</p> <p>Traditionally, the essential oil of aromatic herbs is obtained using hydrodistillation (HD). Because the emitted volatile fraction plays a fundamental role in a plant's life, various novel techniques have been developed for its extraction from plants. Among these, headspace solid phase microextraction (HS-SPME) can be used to obtain a rapid fingerprint of a plant's headspace. <it>Daucus crinitus </it>Desf. is a wild plant that grows along the west coast of Algeria. Only a single study has dealt with the chemical composition of the aerial part oils of Algerian <it>D. crinitus</it>, in which isochavicol isobutyrate (39.0%), octyl acetate (12.3%), and β-caryophyllene (5.4%) were identified. Using GC-RI and GC-MS analysis, the essential oils and the volatiles extracted from separated organs of <it>D. crinitus </it>Desf. were studied using HS-SPME.</p> <p>Results</p> <p>GC-RI and GC-MS analysis identified 72 and 79 components in oils extracted using HD and in the volatile fractions extracted using SPME, respectively. Two types of essential oils were produced by the plant: the root oils had aliphatic compounds as the main component (87.0%-90.1%), and the aerial part oils had phenylpropanoids as the main component (43.1%-88.6%). HS-SPME analysis showed a more precise distribution of compounds in the organs studied: oxygenated aliphatic compounds were well represented in the roots (44.3%-84.0%), hydrocarbon aliphatic compounds were in the leaves and stems (22.2%-87.9%), and phenylpropanoids were in the flowers and umbels (47.9%-64.2%). Moreover, HS-SPME allowed the occurrence of isochavicol (29.6 - 34.7%) as main component in <it>D. crinitus </it>leaves, but it was not detected in the oils, probably because of its solubility in water.</p> <p>Conclusions</p> <p>This study demonstrates that HD and HS-SPME modes could be complimentary extraction techniques in order to obtain the complete characterization of plant volatiles.</p

Spectral Data of Two New Asymmetric Sesquiterpene Alcohols: (14R)-β-Oplopenol and (14S)-β-Oplopenol

Abstract: The epimeric sesquiterpene alcohols (14R)-β-oplopenol and (14S)-β-oplopenol were obtained by LiAlH4 reduction of β-oplopenone. The complete 1 H- and 13 C-NMR assignments of these two new sesquiterpene alcohols have been made using 1D and 2D NMR techniques, including COSY, NOESY, HSQC, HMBC experiments

Sifat Mekanika Bambu Petung Laminasi

Laminated bamboo is a kind of engineered structure to improve mechanical properties of bamboo. It is done in order to meet the need of building materials to the building structure. Laminated bamboo are constructed by gluing a slice bamboo, so it has its advantages can be made in any size and a more uniform mechanical properties of natural bamboo. Mechanical properties is required for structural analysis. Good modeling in structural analysis and numerical properties must be supported by valid mechanical properties. ASTM D143 explains the procedure in getting the mechanical properties of wood . This study refers to ASTM D143 to get laminated bamboo mechanical properties of the material are then arranged in a linear elastic lamina mulitilayer Orthotropic . Proficiency level parameter values for bamboo laminate is Young's Modulus ( E ) , EL = 11 840 MPa ; ER = 511.14 MPa ; ET = 814.39 MPa , Poisson ratio νLR = 0.179 ; νLT = 0.229 ; νRT = 0.231 , and Modulus Slide (G , MPa) GLR = 2.6112 ; GLT = 1.0435 ; GRT = 1.0435

New advances in the volatile metabolites of Frullania tamarisci

International audienceThe chemical composition of Frullania tamarisci essential oil from Corsica was investigated using GC-FID, GC-MS and NMR analyses. The essential oil compositions were further studied analyzing samples prepared from three different Corsican locations and during a seasonal vegetative cycle. 40 components, which accounted for 78.3-89.8% of the total amount of F. tamarisci essential oils were identified. The main components were tamariscol coeluted with pacifigorgiol (30.4-41.5%), germacra-1(10)E,5E-dien-11-ol (3.6-7.1%), gamma-cylocostunolide (1.8-20.1%), gamma-dihydrocyclocostunolide (1.2-8.0%) and frullanolide (1.7-4.9%). Among them, germacra-1(10)E,5E-dien-11-ol was never reported in the Frullania genus and pacifigorgiol, a tamariscol GC-overlapped compound, was for the first time reported in Bryophytes. Finally using 1D and 2D NMR experiments, we reported gamma-dihydrocyclocostunolide as a new natural compound. Two Corsican F. tamarisci essential oils with different GC profiles were assessed for their phytotoxic behavior against onion seeds and seedling in order to evaluate their capacity to inhibit germination and alter the development of plantlets. According to the essential oil-compositions, a stimulation of the onion seedling was observed when sesquiterpene lactones amount was higher, whilst the inhibition of roots growth occurs when sesquiterpene alcohols were predominantly

Volatile constituents of <i>Achillea ligustica</i> All. by HS-SPME/GC/GC-MS: comparison with essential oils obtained by hydrodistillation from Corsica and Sardinia

The volatile components extracted from the headspace (HS) of Achillea ligustica All. samples and their separated organs using solid phase microextraction (SPME) were investigated by gas chromatography and gas chromatography-mass spectrometry. Fiftyseven compounds were identified, the main components were camphor (14.2–29.8%), artemisia ketone (0.3–26.7%), santolina alcohol (0.5–9.4%), camphene (3.0–9.0%) and trans-sabinyl acetate (1.6–5.5%). Moreover, the chemical composition of Corsican and Sardinian A. ligustica oils obtained from flowers and leafy stems harvested in four regions of both islands, were investigated. Two collective oils of A. ligustica were also investigated, comparison between both oils as well as from data literature were reported. A comparison of hydrodistillation and HS-SPME extraction of volatile components in term of isolation time, plant-consuming and chemical composition was discussed. HS-SPME technique was clearly fast in contrast to hydrodistillation (90 min/300 min). HS extraction was performed with a much smaller amount of plant than hydrodistillation. Although the aromatic profiles of HS-fractions and oils showed several quantitative differences HS-SPME can be applied to routine control analysis of aromatic and medicinal plants

Chemotaxonomic investigations of peel and petitgrain essential oils from 17 citron cultivars

International audienceChemical compositions of essential oils from 17 citron cultivars were studied using GC and GC/MS. To the best of our knowledge, chemical compositions of peel and petitgrain oils from seven of them were reported for the first time. Combined analysis of peel and petitgrain essential oils led to the identification of 37 components (amounting to 98.2-99.9% of the total oil) and 42 components (97.0-99.9%), respectively. Statistical analysis was applied to identify possible relationships between citron cultivars. The levels of seven components, i.e., limonene, beta-pinene, gamma-terpinene, neral, geranial, nerol, and geraniol, indicated that the cultivars could be classified in four main chemotypes for peel and petitgrain oils. Chemotaxonomic investigations were carried out to establish relations between the morphological characteristics of citron cultivars and their corresponding oil compositions

Volatile composition of hybrids Citrus juices by headspace solid-phase micro extraction/gas chromatography/mass spectrometry

International audienceThe volatile compounds of Citrus juices have been extracted by headspace solid-phase microextraction (HS-SPME) and analysed by gas chromatography (GC) and gas chromatography/mass spectrometry (GC/MS). This work deals with the analysis of 65 cross pollinated hybrid fruits and their parents: mandarin (Citrus reticulata Blanco var. Willow Leaf) and clementine (Citrus reticulata × Citrus sinensis var. Commune). Among the 44 components identified which accounted for 90.2 to 99.8% of the volatile fraction, limonene (56.8–93.3%) and γ-terpinene (0.1–36.4%) were the major components in all samples. The clementine juice was characterised by the pre-eminence of limonene (90.0%) and a minor amount of γ-terpinene (1.2%) while the mandarin juice exhibited high amount of limonene (66.3%) and γ-terpinene (21.1%). All hybrid juices showed qualitatively similar composition but differing in the quantitative profile of the couple limonene/γ-terpinene. The principal component analysis (PCA) and the discriminant analysis indicated that hybrids samples were symmetrically distributed around the both parents. If some studies were found in the literature about mandarin juice, to our knowledge, this work is the first study on the volatile compounds of clementine juice and a large amount of hybrids

Chemical variability of peel and leaf oils of mandarins

International audiencePeel and leaf oils of 35 mandarin cultivars, belonging to five different species, were obtained from fruits and leaves collected on mandarin-trees kept at the same pedoclimatic and cultural conditions. Their chemical composition was investigated by capillary GC, GC-MS and 13C-NMR and the results were submitted to a statistical analysis. Two major chemotypes, limonene and limonene/y-terpinene, were distinguished for peel oils, while five chemotypes, linalool, sabinene/linalool, β-pinene/linalool, γ-terpinene and methyl N.methylanthranilate, were observed for leaf oils. The results were compared with those already reported by our group

Chemical variability of peel and leaf oils of mandarins

International audiencePeel and leaf oils of 35 mandarin cultivars, belonging to five different species, were obtained from fruits and leaves collected on mandarin-trees kept at the same pedoclimatic and cultural conditions. Their chemical composition was investigated by capillary GC, GC-MS and 13C-NMR and the results were submitted to a statistical analysis. Two major chemotypes, limonene and limonene/y-terpinene, were distinguished for peel oils, while five chemotypes, linalool, sabinene/linalool, β-pinene/linalool, γ-terpinene and methyl N.methylanthranilate, were observed for leaf oils. The results were compared with those already reported by our group

(E)-Methylisoeugenol and elemicin: antibacterial components of Daucus carota L. essential oil against Campylobacter jejuni.

International audienceThe essential oil of wild Daucus carota L. obtained from aerial parts at the end of the flowering stage (DCEO) was reported as antimicrobial against the human enteropathogen Campylobacter jejuni. The aim of the present study was to extend this analysis to other Campylobacter species and to identify the active compounds of the essential oil, subjected to GC, GC-MS, and (13)C NMR analysis. A minimum inhibitory concentration assay was used to quantify the antimicrobial activity of DCEO and the major components, isolated on column chromatography. Growth of all the C. jejuni, Campylobacter coli, and Campylobacter lari strains tested, including one multidrug resistant C. jejuni, was inhibited to the same extent by DCEO. Molecules that were responsible for the antibacterial activity were identified as (E)-methylisoeugenol and elemicin. Moreover, the use of structural analogues of these compounds allowed us to identify important features that may account for the activity