Factors Affecting Late Infertility Examination

Background:  Infertility is the inability of a couple to get pregnant after 12 months of regular sexual intercourse at least 2-3 times a week with­out using contraception. Delay in the examina­tion will have a bad impact considering that in­creas­ing age will affect the success of the hand­ling of infertility, especially in women so it is neces­sary to know the factors that influence the delay of the initial examination in order to be over­come. This study aims to determine the factors that influence the late infertility examin­ation.Subjects and Method: This was an analytic observational study with a cross sectional design. The study was conducted at Sekar Polyclinic, Dr. Moewardi Hospital, Surakarta, Central Java, from June to August 2019. A sample of 90 out­patients was selected by purposive sampling. The depen­­dent variable was late infertility exami­nation. The independent variables were family sup­port, knowledge, accessibility, and education. The data was collected by medical record and question­­naire. The data were analyzed by a multiple logistic regression.Results: Late infertility examination decreased with strong family support (OR= 0.20; 95% CI= 0.06 to 0.62; p= 0.006), high knowledge (OR= 0.29; 95% CI= 0.09 to 0.92; p= 0.036), good accessibility (OR= 0.18; 95% CI= 0.05 to 0.59; p= 0.005), and high education (OR= 3.41; 95% CI= 0.95 to 12.17; p= 0.059).Conclusion: Late infertility examination decreases with strong family support, high know­ledge, good accessibility, and high education.Keywords: infertility examination, family sup­port, accessibility, knowledgeCorrespondence: Uki Retno Budihastuti. Department of Obstetrics and Gynecology, Dr. Moewardi Hospital,Jl. Kolo­nel Sutarto132, Jebres, Surakarta, Central Java. Email: [email protected]. Mobile: 0812­26­56­140.Journal of Maternal and Child Health (2020), 5(2): 206-212https://doi.org/10.26911/thejmch.2020.05.02.1

The success rate of intrauterine insemination in sperm preparation swim-up method at room temperature compared to the incubator temperature

HIGHLIGHTS • There were no significant differences in TMSC, sperm motility, sperm morphology, and DFI in sperm preparation using the swim-up method at 27°C and 37°C. However, this study provided an overview of the average improvement of DFI at 27°C compared to 37°C. • There was no significant difference in the pregnancy rate of IUI in sperm preparation using the swim-up method at 27°C and 37°C.   ABSTRACT Objective: This study aimed to determine the effect of temperature during sperm preparation on total sperm motile count (TMSC), sperm motility, sperm morphology, DNA fragmentation index (DFI), and pregnancy rate. Materials and Methods: A quasi-experimental laboratory study with pre- and post-test control group was conducted at Sekar Fertility Clinic, Dr. Moewardi General Hospital, Surakarta, Indonesia. A total of 20 sperm samples from infertile patients were prepared using the swim-up method at 27°C (group 1) and 37°C (group 2). TMSC, motility, morphology, and DFI examinations were performed. In addition, IUI was performed to confirm pregnancy rate. Sperm DNA fragmentation was determined using Sperm Chromatin Dispersion/SpermFunc DNAf test. Sperm DNA fragmentation was characterized by a halo <30% of the volume of the sperm head. Results: Group 1 had mean TMSC of 13.77 ± 9.30, while group 2 had 14.82 ± 8.82; p=0.218. Group 1 had a motility value 82.25+12.77 and group 2 had 82.55 ± 11.69; p=0.968. The morphological value for group 1 was 11.25 ± 5.15 and group 2 was 11.6 ± 5.34; p=0.626. The mean DFI for group 1 was 17.79 ± 10.88 and group 2 was 18.18 ± 12.95; p=0.765. Pregnancy rate in group 1 was 10% and group 2 was 20%; p=1.000. Conclusion: There were no significant differences in TMSC, sperm motility, sperm morphology, DFI, and pregnancy rate in sperm preparation using the swim-up method at 27°C and 37°C