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CD3- Leukocytes Present in the Human Uterus During Early Placentation: Phenotypic and Morphologic Characterization of the CD56++ Population
In this study, the CD3- LGL/NK cells present in the pregnant human uterus have been
characterized. Phenotypic and morphologic analyses of decidual LGL revealed many similarities
to the minor CD56bright+, CD16- subset in peripheral blood, but there were some
important differences. The relative surface density of CD56+ is greatly increased on decidual
LGL to 22 x that found on the majority of CD56 peripheral blood NK cells. The CD56bright
cells in decidua show LGL morphology, whereas in peripheral blood, they are .mainly
agranular. Proliferation of CD56+ cells occurs predominantly during the nonpregnant secretory
(luteal) phase, indicating these CD56+ uterine LGL do not migrate as terminally differentiated
cells. The appearance of CD56 cells was examined at the ultrastructural level using
immunoelectron microscopy. Cells with phenotypic characteristics of decidual LGL occur in
a higher percentage (1.11%) in the peripheral blood of women of reproductive age than in
men (0.66%). On the basis of these results, it is proposed that the CD56bright+ uterine leukocytes
represent a distinctive, hormonally regulated subset possibly adapted to control human
placentation.Peer Reviewe
Image_1_Interleukin-17 producing cells in swine induced by microbiota during the early postnatal period - a brief research report.jpeg
Interleukin-17A (IL-17) is a pro-inflammatory cytokine involved in the immune response to many pathogens playing also a role in certain chronic and autoimmune diseases. The presented study focused on the early postnatal development of IL-17 producing cells in swine. In agreement with previous studies, αβ T-helper (CD3+CD4+) and γδ T (CD3+TCRγδ+) cells were found to be the major producers of IL-17. In newborn conventional piglets, αβ T-helper cells positive for IL-17 were almost undetectable, but their frequency increased markedly with age in all issues examined, i.e., blood, spleen, and mesenteric lymph nodes (MLN). Additional analyses of CD8 and CD27 expression showed that the main αβ T-helper producers of IL-17 has CD8+CD27- phenotype in all tissues. IL-17 positive CD8+CD27+ αβ T-helper subpopulation was found only in blood and spleen. The production of IL17 in CD8-CD27+ αβ T-helper cells was always minor. In contrast, γδ T cells positive for IL-17 did not show a similar age-dependent increase in blood and spleen, whereas they increased in MLN. Because of the age-dependent increase in conventional animals, we included a comparison with germ-free piglets to show that the increase in IL-17 positive cells was clearly depended on the presence of the microbiota as the production in germ-free animals was negligible without any age-dependent increase.</p
Image_2_Interleukin-17 producing cells in swine induced by microbiota during the early postnatal period - a brief research report.png
Interleukin-17A (IL-17) is a pro-inflammatory cytokine involved in the immune response to many pathogens playing also a role in certain chronic and autoimmune diseases. The presented study focused on the early postnatal development of IL-17 producing cells in swine. In agreement with previous studies, αβ T-helper (CD3+CD4+) and γδ T (CD3+TCRγδ+) cells were found to be the major producers of IL-17. In newborn conventional piglets, αβ T-helper cells positive for IL-17 were almost undetectable, but their frequency increased markedly with age in all issues examined, i.e., blood, spleen, and mesenteric lymph nodes (MLN). Additional analyses of CD8 and CD27 expression showed that the main αβ T-helper producers of IL-17 has CD8+CD27- phenotype in all tissues. IL-17 positive CD8+CD27+ αβ T-helper subpopulation was found only in blood and spleen. The production of IL17 in CD8-CD27+ αβ T-helper cells was always minor. In contrast, γδ T cells positive for IL-17 did not show a similar age-dependent increase in blood and spleen, whereas they increased in MLN. Because of the age-dependent increase in conventional animals, we included a comparison with germ-free piglets to show that the increase in IL-17 positive cells was clearly depended on the presence of the microbiota as the production in germ-free animals was negligible without any age-dependent increase.</p