Supplementary Video 16 source data

This dataset is part of the 'Source data and representative raw data for "Peptidoglycan synthesis drives a single population of septal cell wall synthases during division in Bacillus subtilis"' collection. For a link to the related paper, study-level information and guide to the data, as well as viewing all other related research data, please visit the collection. 231117_sh203_phmm_30c_flatpad_slide2_008 is the raw video file used to make Svid 16 (Supplementary_Video_16_SFigure17_FtsZ_G106S).</p

Representative data for rich media, 37C

This dataset is part of the 'Source data and representative raw data for "Peptidoglycan synthesis drives a single population of septal cell wall synthases during division in Bacillus subtilis"' collection. For a link to the related paper, study-level information and guide to the data, as well as viewing all other related research data, please visit the collection. This is a raw video file representative of data collected in rich media at 37C (no perturbations). There are two channels: a 'green' channel (488 nm excitation) showing GFP-FtsZ, and a 'red' channel (561 nm excitation) showing single-molecules of HaloTag-PBP2B.</p

Representative raw data for HT-PBP2B motion in horizontal cells with PC190723 treatment

This dataset is part of the 'Source data and representative raw data for "Peptidoglycan synthesis drives a single population of septal cell wall synthases during division in Bacillus subtilis"' collection. For a link to the related paper, study-level information and guide to the data, as well as viewing all other related research data, please visit the collection. This is a raw video file representative of data collected in rich media at 30C (with PC190723 perturbation). There are two channels: a 'green' channel (488 nm excitation) showing GFP-FtsZ, and a 'red' channel (561 nm excitation) showing single-molecules of HaloTag-PBP2B.</p

Extended Data Figure 9 source data

This dataset is part of the 'Source data and representative raw data for "Peptidoglycan synthesis drives a single population of septal cell wall synthases during division in Bacillus subtilis"' collection. For a link to the related paper, study-level information and guide to the data, as well as viewing all other related research data, please visit the collection. 220303_slide2_5_MMStack_Default_561_denoise.ome_ring6, 220303_slide2_5_MMStack_Default_561_denoise.ome_ring6_kymo, and 230619_220303_slide2_6_ring4_kymo_intensity correspond to ED Fig. 9a. 220303_slide2_6_MMStack_Default_561_denoise.ome_ring4, 220303_slide2_6_MMStack_Default_561_denoise.ome_ring4_kymo, and 230619_220303_slide2_6_ring4_kymo_intensity correspond to ED Fig. 9b. 220309_slide2_4_MMStack_Default_561_denoise.ome_ring9, 220309_slide2_4_MMStack_Default_561_denoise.ome_ring9_kymo, and 230619_220309_slide2_4_ring9_kymo_intensity correspond to ED Fig. 9c. 220310_5_MMStack_Default_561_denoise.ome_ring23, 220310_5_MMStack_Default_561_denoise.ome_ring23_kymo, and 230619_220310_5_ring23_kymo_intensity correspond to ED Fig. 9d.</p

Supplementary Video 2 source data

This dataset is part of the 'Source data and representative raw data for "Peptidoglycan synthesis drives a single population of septal cell wall synthases during division in Bacillus subtilis"' collection. For a link to the related paper, study-level information and guide to the data, as well as viewing all other related research data, please visit the collection. 220316_5_MMStack_Default_561_denoise.ome_ring14 is the raw video file used to produce Svid 2 (Supplementary_Video_2_Figure_1c_bottom)</p

Supplementary Video 5 source data

This dataset is part of the 'Source data and representative raw data for "Peptidoglycan synthesis drives a single population of septal cell wall synthases during division in Bacillus subtilis"' collection. For a link to the related paper, study-level information and guide to the data, as well as viewing all other related research data, please visit the collection. 220809_sh147_phmm_30c_vercini_fosfomycin_slide2_006_561_denoise_ring1 is the raw video file used to make Svid 5 (Supplementary_Video_5_Figure_1e_top).</p

Extended Data Figure 6 source data

This dataset is part of the 'Source data and representative raw data for "Peptidoglycan synthesis drives a single population of septal cell wall synthases during division in Bacillus subtilis"' collection. For a link to the related paper, study-level information and guide to the data, as well as viewing all other related research data, please visit the collection. 231113_sh147_phmm_30c_flatpad_slide2_006_cut_track_f52 corresponds to ED Fig. 6a. 231113_sh147_phmm_30c_flatpad_slide2_006_cut_cut_track_f52 corresponds to ED Fig. 6b. 231113_sh147_phmm_30c_flatpad_slide2_006_septum28_fov corresponds to ED Fig. 6c. 231113_sh147_phmm_30c_flatpad_slide2_006_septum28_fov_rot corresponds to ED Fig. 6d. 231118_off_axis_residuals_violindabest corresponds to ED Fig. 6e. Whitley_2024_ED_Fig6_SourceData contains all the data to produce ED Fig. 6e.</p

Supplementary Figure 1 source data

This dataset is part of the 'Source data and representative raw data for "Peptidoglycan synthesis drives a single population of septal cell wall synthases during division in Bacillus subtilis"' collection. For a link to the related paper, study-level information and guide to the data, as well as viewing all other related research data, please visit the collection.  2023-12-05_Morphology_SH211_005 is the raw image file behind Supplementary Figure 1a. 231028_sh147_phmm_30c_100uM_iptg_0p075pct_xyl_nilered_008 is the raw image file behind Supplementary Figure 1b. 231207_sh211_sh147_lengths_nilered_jgdata_231205 is the figure with data behind Supplementary Figure 1c. 231208_sh147_morphology_xylose is the figure with data behind Supplementary Figure 1d. Whitley_2024_SFig1_SourceData is an excel file with cell lengths corresponding to data in SFigs 1c and 1d (one sheet for each figure panel).</p

Figure 3 source data

This dataset is part of the 'Source data and representative raw data for "Peptidoglycan synthesis drives a single population of septal cell wall synthases during division in Bacillus subtilis"' collection. For a link to the related paper, study-level information and guide to the data, as well as viewing all other related research data, please visit the collection.  220405_slide2_6_MMStack_Default_561_denoise.ome_ring11 corresponds to Fig. 3a, top kymograph. 220407_slide2_3_MMStack_Default_561_denoise.ome_ring18 corresponds to Fig. 3a, bottom kymograph. 220928_sh147sh056_phmm_30c_vercini_slide2_561_denoise_ring3 corresponds to Fig. 3c, top kymograph. 221013_sh147sh056_phmm_30c_vercini_003_561_denoise_ring1 corresponds to Fig. 3c, bottom kymograph. 230613_sh147_phmm_30c_pc19_3gauss and 230607_sh147_phmm_30c_PC19_hist_logscale correspond to Fig. 3b. 230613_sh147sh056_phmm_30c_3gauss and 230607_sh147_phmm_30c_G106S_hist_logscale correspond to Fig. 3d. 231130_FtsZ_vs_PBP2B_speeds_95ci corresponds to Fig. 3e. Whitley_2024_Fig3_SourceData contains all data used to produce plots in Fig. 3.</p

Figure 4 source data

This dataset is part of the 'Source data and representative raw data for "Peptidoglycan synthesis drives a single population of septal cell wall synthases during division in Bacillus subtilis"' collection. For a link to the related paper, study-level information and guide to the data, as well as viewing all other related research data, please visit the collection.  220316_5_MMStack_Default_561_denoise.ome_ring14, 220316_5_MMStack_Default_561_denoise.ome_ring14_kymo, and 230607_220316_5_ring14_kymo_intensity correspond to Fig. 4a. 220316_3_MMStack_Default_561_denoise.ome_ring11, 220316_3_MMStack_Default_561_denoise.ome_ring11_kymo, and 230607_220316_3_ring11_kymo_intensity correspond to Fig. 4b. 220303_slide2_8_MMStack_Default_561_denoise.ome_ring22, 220303_slide2_8_MMStack_Default_561_denoise.ome_ring22_kymo, and 230607_220303_slide2_8_ring22_kymo_intensity correspond to Fig. 4c. 220303_slide2_5_MMStack_Default_561_denoise.ome_ring6, 220303_slide2_5_MMStack_Default_561_denoise.ome_ring6_kymo, and 230607_220303_slide2_5_ring6_kymo_intensity correspond to Fig. 4d.</p