ЭЛЕМЕНТНЫЙ АНАЛИЗ ВОЗДУШНЫХ АЭРОЗОЛЕЙ

Layers of aerosols, deposited on a high volume fiberglass filter from air, were analyzed using proton beam. Both the RBS spectrum and PIXE one were stored simultaneously, which allows one to calculate the light element content as well as to identify heavier elements and calculate their concentrations in aerosols.Элементный анализ воздушных аэрозолей, нанесенных на фибергласовые фильтры, был проведен с помощью пучка протонов с энергией 2 МэВ. Одновременно с набором спектров характеристического рентгеновского излучения регистрировались спектры обратно рассеянных протонов, что позволило определить концентрации легких элементов в составе аэрозолей, а кроме того, однозначно идентифицировать тяжелые примеси и вычислить их концентрацию

Deficient Induction Response in a Xenopus Nucleocytoplasmic Hybrid

Defects in induction signaling and response underlie the nucleocytoplasmic incompatibility between two evolutionarily distant frog species, while specific treatments partially restore this response in explants and whole embryos

Analysis of Imprinted Gene Expression in Normal Fertilized and Uniparental Preimplantation Porcine Embryos

In the present study quantitative real-time PCR was used to determine the expression status of eight imprinted genes (GRB10, H19, IGF2R, XIST, IGF2, NNAT, PEG1 and PEG10) during preimplantation development, in normal fertilized and uniparental porcine embryos. The results demonstrated that, in all observed embryo samples, a non imprinted gene expression pattern up to the 16-cell stage of development was common for most genes. This was true for all classes of embryo, regardless of parental-origins and the direction of imprint. However, several differentially expressed genes (H19, IGF2, XIST and PEG10) were detected amongst the classes at the blastocyst stage of development. Most interestingly and despite the fact that maternally and paternally expressed genes should not be expressed in androgenones and parthenogenones, respectively, both uniparental embryos expressed these genes when tested for in this study. In order to account for this phenomenon, we compared the expression patterns of eight imprinted genes along with the methylation status of the IGF2/H19 DMR3 in haploid and diploid parthenogenetic embryos. Our findings revealed that IGF2, NNAT and PEG10 were silenced in haploid but not diploid parthenogenetic blastocysts and differential methylation of the IGF2/H19 DMR3 was consistently observed between haploid and diploid parthenogenetic blastocysts. These results appear to suggest that there exists a process to adjust the expression status of imprinted genes in diploid parthenogenetic embryos and that this phenomenon may be associated with altered methylation at an imprinting control region. In addition we believe that imprinted expression occurs in at least four genes, namely H19, IGF2, XIST and PEG10 in porcine blastocyst stage embryos

ELEMENTAL ANALYSIS OF AIR AEROSOLS

Layers of aerosols, deposited on a high volume fiberglass filter from air, were analyzed using proton beam. Both the RBS spectrum and PIXE one were stored simultaneously, which allows one to calculate the light element content as well as to identify heavier elements and calculate their concentrations in aerosols

Assessment of MDA efficiency for genotyping using cloned embryo biopsies

Abstract not availableAndrea Lauri, Giovanna Lazzari, Cesare Galli, Irina Lagutina, Elena Genzini, Francesco Braga, Paola Mariani, John L. William