132 research outputs found
Patterning of dielectric nanoparticles using dielectrophoretic forces generated by ferroelectric polydomain films
A theoretical study of a dielectrophoretic force, i.e. the force acting on an
electrically neutral particle in the inhomogeneous electric field, which is
produced by a ferroelectric domain pattern, is presented. It has been shown by
several researchers that artificially prepared domain patterns with given
geometry in ferroelectric single crystals represent an easy and flexible method
for patterning dielectric nanoobjects using dielectrophoretic forces. The
source of the dielectrophoretic force is a strong and highly inhomogeneous
(stray) electric field, which exists in the vicinity of the ferroelectric
domain walls at the surface of the ferroelectric film. We analyzed
dielectrophoretic forces in the model of a ferroelectric film of a given
thickness with a lamellar 180 domain pattern. The analytical formula
for the spatial distribution of the stray field in the ionic liquid above the
top surface of the film is calculated including the effect of free charge
screening. The spatial distribution of the dielectrophoretic force produced by
the domain pattern is presented. The numerical simulations indicate that the
intersection of the ferroelectric domain wall and the surface of the
ferroelectric film represents a trap for dielectric nanoparticles in the case
of so called positive dielectrophoresis. The effects of electrical neutrality
of dielectric nanoparticles, free charge screening due to the ionic nature of
the liquid, domain pattern geometry, and the Brownian motion on the mechanism
of nanoparticle deposition and the stability of the deposited pattern are
discussed.Comment: Accepted in the Journal of Applied Physics, 10 pages, 5 figure
Pressure on charged domain walls and additional imprint mechanism in ferroelectrics
The impact of free charges on the local pressure on a charged ferroelectric
domain wall produced by an electric field has been analyzed. A general formula
for the local pressure on a charged domain wall is derived considering full or
partial compensation of bound polarization charges by free charges. It is shown
that the compensation can lead to a very strong reduction of the pressure
imposed on the wall from the electric field. In some cases this pressure can be
governed by small nonlinear effects. It is concluded that the free charge
compensation of bound polarization charges can lead to substantial reduction of
the domain wall mobility even in the case when the mobility of free charge
carriers is high. This mobility reduction gives rise to an additional imprint
mechanism which may play essential role in switching properties of
ferroelectric materials. The effect of the pressure reduction on the
compensated charged domain walls is illustrated for the case of 180-degree
ferroelectric domain walls and of 90-degree ferroelectric domain walls with the
head-to-head configuration of the spontaneous polarization vectors.Comment: subm. to PRB. This verion is extended by appendi
First-principles investigation of 180-degree domain walls in BaTiO_3
We present a first-principles study of 180-degree ferroelectric domain walls
in tetragonal barium titanate. The theory is based on an effective Hamiltonian
that has previously been determined from first-principles
ultrasoft-pseudopotential calculations. Statistical properties are investigated
using Monte Carlo simulations. We compute the domain-wall energy, free energy,
and thickness, analyze the behavior of the ferroelectric order parameter in the
interior of the domain wall, and study its spatial fluctuations. An abrupt
reversal of the polarization is found, unlike the gradual rotation typical of
the ferromagnetic case.Comment: Revtex (preprint style, 13 pages) + 3 postscript figures. A version
in two-column article style with embedded figures is available at
http://electron.rutgers.edu/~dhv/preprints/index.html#pad_wal
Domain geometry engineering and domain average engineering in ferroics
Abstract Multidomain samples of ferroics (ferroelectrics, ferroelastics, and related materials) with fixed geometrical distribution of domains can offer new macroscopic properties required for particular applications. Two extreme cases of such applications are defined. In domain-geometry-engineered samples of ferroic crystals, the spatial distribution of domains and thus the spatial distribution of tensorial properties is tuned to correspond to the k-vectors of applied electric, optical or acoustic fields. For a given wavelength, the size, geometry, and distribution of domains give rise to a qualitatively new kind of response specified by the symmetry of the multidomain system. In domainaverage-engineered samples of ferroic crystals, the specimen is subdivided into a very large number of domains, representing µ domain states where µ is smaller than the theoretically allowed maximum number, and forming a regular or irregular pattern. Its response to external fields is roughly described by tensorial properties averaged over all of the domain states involved. The effective symmetry of the domain-average-engineered system is given by a point group H and we show how it can be determined. As an example, all groups H are specified for domain-average-engineered samples which can arise in a material undergoing the phase transition with symmetry change from m3m to 3m. Ferroic materials (and here we concentrate on non-magnetic materials, i.e. on ferroelectrics, ferroelastics, and higher-order ferroics) play an essential role in a number of technical applications. In some of them, dynamic domain processes are essential (e.g. thin-film memories, electron emitters) while in others the static distribution domains in the sample play the crucial role. In this contribution we concentrate on the latter case and wish to specify a clear distinction between two kinds of such static multidomain system. We have in mind materials undergoing a structural phase transition from the parent phase of point group G into the ferroic phase of symmetry F ⊂ G (such a material is referred t
A bispecific chimeric antigen receptor molecule enhances T cell activation through dual immunological synapse formation and offsets antigen escape in glioblastoma
Background
Antigen escape tumor cell variants prevail in tumors recurring after treatment with chimeric antigen receptor (CAR) T cells with a single specificity. Recurrent tumors preserve alternative non-targeted tumor associated antigens.
Hypothesis
A bispecific CAR will mitigate antigen escape enhancing the antitumor activity of T cells.
Methods and results
HER2 and IL13Rα2 are currently targeted in Phase I glioblastoma (GBM) trials using CAR T cells. We created a bispecific CAR molecule with a HER2-specific scFv joined in tandem to an IL13Rα2-binding moiety in the CAR exodomain (Tandem CAR) and a CD28.ζ signaling endodomain. We used computational modeling to interrogate this design. GBM patients' Tandem CAR T cells showed distinct binding to soluble HER2 and IL13Rα2 and killed primary autologous GBM cells. Three-dimensional reconstitution and quantification of confocal images of the Tandem CAR T cell/tumor interface revealed enhanced bifunctional immunological synapses compared to conventional CARs. Further, Tandem CAR T cells exhibited significantly enhanced inexhaustible activation dynamics when compared to conventional HER2 or IL13Rα2 CAR T cells and better controlled established GBM in an orthotopic murine model by offsetting both HER2 and IL13Rα2 escape.
Conclusion
Tandem chimeric antigen receptors enhance T cell activation and mitigate antigen escape through bifunctional immunological synapse formation in GBM
The Plastid Genome of Eutreptiella Provides a Window into the Process of Secondary Endosymbiosis of Plastid in Euglenids
Euglenids are a group of protists that comprises species with diverse feeding modes. One distinct and diversified clade of euglenids is photoautotrophic, and its members bear green secondary plastids. In this paper we present the plastid genome of the euglenid Eutreptiella, which we assembled from 454 sequencing of Eutreptiella gDNA. Comparison of this genome and the only other available plastid genomes of photosynthetic euglenid, Euglena gracilis, revealed that they contain a virtually identical set of 57 protein coding genes, 24 genes fewer than the genome of Pyramimonas parkeae, the closest extant algal relative of the euglenid plastid. Searching within the transcriptomes of Euglena and Eutreptiella showed that 6 of the missing genes were transferred to the nucleus of the euglenid host while 18 have been probably lost completely. Euglena and Eutreptiella represent the deepest bifurcation in the photosynthetic clade, and therefore all these gene transfers and losses must have happened before the last common ancestor of all known photosynthetic euglenids. After the split of Euglena and Eutreptiella only one additional gene loss took place. The conservation of gene content in the two lineages of euglenids is in contrast to the variability of gene order and intron counts, which diversified dramatically. Our results show that the early secondary plastid of euglenids was much more susceptible to gene losses and endosymbiotic gene transfers than the established plastid, which is surprisingly resistant to changes in gene content
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