Gene expression of selected apoptotic markers in Human Oral Carcinoma (HSC-3) cell lines trewated with myrmecodia pendans plant extract

Introduction: Myrmecodia pendans (M. pendans), also known as Sarang Semut is an epiphyte with potential as a new therapeutic agent. Recently, we reported that M. pendans induced apoptotic activity in human oral squamous carcinoma (HSC-3) cell line. This study aimed to study how M. pendans extract affected apoptotic markers BAX/BCL-2 expression. Materials and methods: M. pendans was purchased from West Papua, Indonesia. The hypocotyl was allowed to dry thoroughly, then extracted aqueously. The extract was kept at -80 °C before freeze drying. The crude extract was used in the experiments. HSC-3 cells were cultured in Dulbecco’s Modified Essential Medium, then exposed to M. pendans at 2.5, 5.0 and 7.5 mg/ml for 24 and 48 h. Doxorubicin (0.005 mg/ml) was used as positive control. BAX and BCL-2 expression was analysed by Quantitative Real Time Polymerase Chain Reaction. Results: BAX and BCL-2 expression in HSC-3 cells was affected by M. pendans. At 24 h, highest levels of BAX and BCL-2 were observed with M. pendans at 2.5 mg/ml, while downregulation was observed at 5 mg/ml. However, after 48 h, BAX and BCL-2 were downregulated at all concentrations. The ratio of BAX/ BCL-2 in HSC-3 cells at 24 h showed significant upregulation at all treatment concentrations, but was downregulated after 48 h. Conclusion: M. pendans extract induced apoptotic activity in HSC-3 cells. This study suggests that induction of apoptosis in HSC-3 cell line is regulated via the pro-apoptotic (BAX) and anti- apoptotic (BCL -2) pathways

Gene Expression of Selected Apoptotic Markers in Human Oral Squamous Carcinoma HSC-3 Cell Line Treated with Myrmecodia Pendans Plant

This poster was submitted to participate at the 11th Annual ISCC Conference and Congress organised by the Indonesian Society of Cancer and Chemopreventive, held on 9-21 November 2020

Biology 1 (bio0415) workbook

Biology I (Bio415) Workbook for BIO0415 course. This book is specially written for foundation students taking Biology at Centre for Foundation Studies, IIUM. The purpose of this workbook is to provide students with exercises to help them understand topics in Biology This Biology I Workbook consists of multiple-choice questions and structure questions for 14 chapters. Answer schemes and feedbacks are provided at the end of each chapter as guidance for the students to understand the concepts. The questions in each chapter were tested on students and moderated to confirm the validity

Biology 1 workbook

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