Western hybridization performed using anti-PpuR antibody on total cellular protein extracts

<p><b>Copyright information:</b></p><p>Taken from "The Lon protease is involved in -acyl homoserine lactone quorum sensing regulation"</p><p>http://www.biomedcentral.com/1471-2180/7/71</p><p>BMC Microbiology 2007;7():71-71.</p><p>Published online 26 Jul 2007</p><p>PMCID:PMC1949823.</p><p></p> Total protein extracts were performed using different protein amounts of either parent strain WCS358 or mutant derivative IBE4 each carrying a plasmid expressing PpuR (pBBRppuR). Lane 1 corresponds to 10 μg of purified PpuR-6His; lane 2 and lane 3 correspond to total proteins from 2 × 10cfu of WCS358(pBBRppuR) and IBE4(pBBRppuR) respectively; lanes 4 and 5 correspond to total proteins from 10cfu. For lanes 4 and 5 the images were scanned using a Versadoc (Biorad) and the QuantityOne software; results of this quantification are displayed as a histogram. See text for all details

TLC analysis of C12-3-oxo-AHL produced by parent strain WCS358 (lane 1), by mutant derivative IBE4 (lane 2) and mutant derivative carrying a plasmid expressing Lon IBE4 (pBBRlon) (lane 3)

<p><b>Copyright information:</b></p><p>Taken from "The Lon protease is involved in -acyl homoserine lactone quorum sensing regulation"</p><p>http://www.biomedcentral.com/1471-2180/7/71</p><p>BMC Microbiology 2007;7():71-71.</p><p>Published online 26 Jul 2007</p><p>PMCID:PMC1949823.</p><p></p> Lane 4 contains the standards C10-3-oxo-AHL and C12-3-oxo-AHL. The AHLs were visualized with AHL-sensor strain (pSB1075); a volume corresponding to 5 × 10cfu was loaded on the TLC assay