39 research outputs found
Ultrastructural observations on the microvasculature in advanced gastric carcinomas
The ultrastructural features associated with
vascular permeability in 9 cases of advanced gastric
carcinomas were studied, and compared with that of
control non-neoplastic mucosa. Tumour rnicrovasculature
showed features in common with those of
control mucosa, including complete basa1 lamina, welldeveloped
interendothelial junctions, fenestrations and
caveolae. Some tumour blood vessels showed
endothelial cell swelling accompained by luminal
narrowing and perivascular fibrosis. In 2 out of 9 cases,
there were endothelial attenuation with numerous
fenestrations and vesiculo-vacuolar organelles. The
vesiculo-vacuolar organelle is a recently described
cytoplasmic structure found in the endothelial cells
lining turnour microvessels and normal venules and
which provides an important pathway for extravasation
of circulating macromolecules. Our ultrastructural data
suggest that advanced gastric carcinomas share with
experimental tumour models in vivo only some
morphologic features associated with hyperpermeability
including fenestration, endothelial attenuation and
vesiculo-vacuolar organelles. The implications of
perivascular fibrosis on the delivery of immune cells to
gastric carcinomas are discussed
Ultrastructural observations on the microvasculature in advanced gastric carcinomas
The ultrastructural features associated with vascular permeability in 9 cases of advanced gastric carcinomas were studied, and compared with that of control non-neoplastic mucosa. Tumour microvasculature showed features in common with those of control mucosa, including complete basa1 lamina, welldeveloped interendothelial junctions, fenestrations and caveolae. Some tumour blood vessels showed endothelial cell swelling accompained by luminal narrowing and perivascular fibrosis. In 2 out of 9 cases, there were endothelial attenuation with numerous fenestrations and vesiculo-vacuolar organelles. The vesiculo-vacuolar organelle is a recently described cytoplasmic structure found in the endothelial cells lining turnour microvessels and normal venules and which provides an important pathway for extravasation of circulating macromolecules. Our ultrastructural data suggest that advanced gastric carcinomas share with experimental tumour models in vivo only some morphologic features associated with hyperpermeability including fenestration, endothelial attenuation and vesiculo-vacuolar organelles. The implications of perivascular fibrosis on the delivery of immune cells to gastric carcinomas are discussed
Minute and small early gastric carcinoma with special reference to eosinophil infiltration
The authors examined 25 minute early
gastric cancers (EGC) and 13 small EGC in order to
investigate the incidence and possible causes for the
infiltration of eosinophils. The degree of eosinophil
infiltration was higher in tumour stroma than in adjacent
normal-appearing mucosa; this correlation was
statistically significant (P < 0.001). Tumour-associated
tissue eosinophilia (TATE) was not correlated with size,
histological type, necrosis of the tumour nor gastritis
activity in adjacent non-tumoral mucosa. Electron
microscopy, performed in 4 cases of small EGC, showed
tumour stromal eosinophils with morphological evidence
of activation similar to those described for tissue
eosinophils in various disorders. Some tumour cells in
intimate contact with activated eosinophils exhibited
foca1 cytopathic changes. TATE represents local
inflammation probably leading to tumour cell damage.
The immunological role of the eosinophils against
tumour cells in vivo deserves further investigation
Neutrophil interaction with tumour cells in small early gastric cancer, ultrastructural observations
The authors studied the neutrophil
inflammatory reaction by light and electron microscopy
in 4 small early gastric cancers (three tubular adenocarcinomas
and one adenocarcinoma with signet-ring
cell component), selected for the absence of coagulative
necrosis and ulceration. Neutrophils showed ultrastructural
signs of activation such as aggregation,
adhesion and lipid bodies. Some neutrophils were found
to be in intimate contact with the intact tumour cells and
with those that displayed a varying degree of damage.
In particular, relatively early damage, such as
disorganization of the intermediate filaments and
dilatation of the rough endoplasmic reticulum, occurred
at the contact regions. In spite of the severe damage to
the tumour cells, the neutrophils themselves were intact.
These findings are discussed in relationship to a number
of recent studies of the neutrophil-mediated tumour cell
injury, in man and animals
Ultrastructural study of the vascular response in small early gastric cancer
The microvasculature of the stroma of four
cases of small early gastric cancer (EGC) was
investigated by conventional electron microscopy.
Severe damage to small and large fenestrated capillaries
was observed around endothelium-adherent, partially
degranulated neutrophils. The findings suggest the
existence of neutrophil-mediated injury of endothelial
cells during the development of inflammatory responses
in small EGC. The severely injured microvessels
exhibited increase in vasopermeability, microhaemorrhage,
and platelet aggregates. Other microvascular
changes included endothelial cell and pericyte
activation as well as basal lamina replications, indicative
of repeated episodes of endothelial injury, necrosis and
regeneration. This new capillary growth within the old
basal laminas shared morphological features with a
peculiar angiogenic process described in man and
animals
Multimodal imaging in Susac syndrome: A case report and literature review
7noSusac syndrome (SS) is a rare microangiopathy that involves arterioles of the brain, retina, and cochlea. Diagnosis is extremely difficult because of the rarity of the disease and because the signs and symptoms often occur at different times. Multidisciplinary approaches and multimodal images are mandatory for diagnosis and prompt therapy. In this report, we describe a case of SS and the application of multimodal retinal imaging to evaluate the ophthalmologic changes and to confirm diagnosis. Early diagnosis and therapy based on the associations of steroids and immunosuppressants are necessary to limit the sequelae of the disease.openopenBagaglia S.A.; Passani F.; Oliverio G.W.; Inferrera L.; Menna F.; Meduri A.; Mazzotta C.Bagaglia, S. A.; Passani, F.; Oliverio, G. W.; Inferrera, L.; Menna, F.; Meduri, A.; Mazzotta, C