Lethal Dose 50% (LD50) values and virulence determinants

In the publication "Genome Sequence of Two Novel Virulent Clinical Strains of Burkholderia pseudomallei Isolated from Acute Melioidosis Cases Imported to Israel from India and Thailand" to which the online resource pertains, we report the genomes of two novel clinical isolates (MWH2021 and MST2022) of Burkholderia pseudomallei identified in two distinct acute cases of melioidosis diagnosed in two individuals arriving to Israel from India and Thailand, respectively. The publication includes preliminary genetic analysis of the genomes determining their phylogenetic classification in rapport to the genomes of other Burkholderia pseudomallei strains documented in the NCBI database. Inspection of the genetic data established the presence or absence of loci encoding for several important virulence determinants involved in the molecular pathogenesis of melioidosis. Virulence analysis in murine models of acute or latent melioidosis established that both strains belong to the highly virulent class of Burkholderia pseudomalleii.</p

Supplementary Method Data

In the publication "Genome Sequence of Two Novel Virulent Clinical Strains of Burkholderia pseudomallei Isolated from Acute Melioidosis Cases Imported to Israel from India and Thailand" to which the online resource pertains, we report the genomes of two novel clinical isolates (MWH2021 and MST2022) of Burkholderia pseudomallei identified in two distinct acute cases of melioidosis diagnosed in two individuals arriving to Israel from India and Thailand, respectively. The publication includes preliminary genetic analysis of the genomes determining their phylogenetic classification in rapport to the genomes of other Burkholderia pseudomallei strains documented in the NCBI database. Inspection of the genetic data established the presence or absence of loci encoding for several important virulence determinants involved in the molecular pathogenesis of melioidosis. Virulence analysis in murine models of acute or latent melioidosis established that both strains belong to the highly virulent class of Burkholderia pseudomalleii.</p

Figure 1. <b>Phylogenetic tree </b><b><i>Burkholderia pseudomallei</i></b><b> strains</b>

Phylogenetic tree of Burkholderia pseudomallei (BP) strains. The genetic similarity of the strains.BP1, BP2, MAA2018, MWH2021 and MST 2022 to other BP strains was determined by comparative sequence analysis of all complete genomes of 128 BP strains available in the NCBI data base as of June 2023. The five clinical strains BP1, BP2, MAA2018, MWH2021 and MST 2022 were isolated from Melioidosis cases diagnosed in Israel in the past 6 years. See references 1 and 2 (below) for BP1, BP2 and MAA2018 (NCBI accession number SAMN11081001). The genomes of the novel clinical strains MWH2021 and MST2022 are available in the NCBI data base as: GCF_030913145.1 and GCA_030144945.1, respectively.Israeli O, Cohen-Gihon I, Brosh-Nissimov T, Zvi A, Beth-Din A, Shifman O, Israeli M, Elia U, Lazar S, Bar-Haim E, Cohen O, Chitlaru T. Draft Genome Sequence of a Rare Israeli Clinical Isolate of Burkholderia pseudomallei. Microbiol Resour Announc. 2019 May 9;8(19):e00281-19. doi: 10.1128/MRA.00281-19. Erratum in: Microbiol Resour Announc. 2019 Oct 3;8(40): PMID: 31072902; PMCID: PMC6509527.Brosh-Nissimov T, Grupel D, Abuhasira S, Leskes H, Israeli M, Lazar S, Elia U, Israeli O, Beth-Din A, Bar-Haim E, Cohen-Gihon I, Zvi A, Cohen O, Chitlaru T. Case Report: Imported Melioidosis from Goa, India to Israel, 2018. Am J Trop Med Hyg. 2019 Sep;101(3):580-584. doi: 10.4269/ajtmh.19-0303. Erratum in: Am J Trop Med Hyg. 2019</p

Data file 6: Genes downregulated upon exposure to ciprofloxacin

 Genes downregulated upon exposure to 0.5×MIC or 1.0×MIC of ciprofloxacin </p

Data file 2: Number of genes modulated upon exposure to doxycycline or ciprofloxacin

   Histograms representing the number of genes modulated upon exposure to 0.5 x MIC (left panel) or 1 x MIC (right panel) concentrations of ciprofloxacin (blue bars) or doxycycline (orange bars).   </p

Data file 5: Genes upregulated upon exposure to ciprofloxacin

Genes upregulated upon exposure to 0.5×MIC or 1.0×MIC of ciprofloxacin </p

Data file 1: Materials and methods

Materials and methods </p

Data file 3: Genes upregulated upon exposure to doxycycline

Genes upregulated upon exposure to 0.5×MIC or 1.0×MIC of doxycycline</p

Data file 4: Genes downregulated upon exposure to doxycycline

  Genes downregulated upon exposure to 0.5×MIC or 1.0×MIC of doxycycline </p