Additional file 1: of Utilization of surgical safety checklists by urological surgeons in Germany: a nationwide prospective survey

Examples of different surgical safety checklists used to improve patient safety. A: World Health Organization Surgical Safety Checklist, B: Surgical Safety Checklist used in Canada, C: Colorado hospital association Surgical Safety Checklist used in Colorado/USA, D: Surgical Safety Checklist used by University Hospital Frankfurt in Germany. (PDF 711 kb

Effect of VPA on tumor volume in Caki-1 xenografts in mice.

<p>Mice in the treatment arm received 200 mg VPA/kg once daily. Control mice received solvent (n = 6). *indicates significant difference to the control mice.</p

Western Blot analysis of cell cycle regulating (fig. 2A) and cell signaling proteins (fig. 2B) in tumor tissue from drug-sensitive (responders) versus drug-resistant mice (non-responders).

<p>Control tissue specimens were taken from untreated animals. Cell lysates (50 µg) were subjected to SDS-PAGE and blotted on the membrane incubated with the corresponding monoclonal antibodies. β-actin served as the internal control. The figure shows one representative from three separate experiments.</p

Long-term exposure of VPA causes drug resistance and Akt up-regulation.

<p><a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0053100#pone-0053100-g004" target="_blank">Fig. 4A</a>: Caki-1 cells were treated for 2 weeks (short-term) or 12 weeks (long-term) with 1 mM VPA, and cell growth was analysed by the MTT assay. Controls remained untreated. The figure shows one representative from six separate experiments. *indicates significant difference to controls. <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0053100#pone-0053100-g004" target="_blank">Fig. 4B</a>: To evaluate Akt expression and activity, Caki-1 cells were treated short-term or long-term with 1 mM VPA. Controls remained untreated. Cell lysates were subjected to SDS-PAGE and blotted on the membrane incubated with the respective monoclonal antibodies. β-actin served as the internal control. One representative from three separate experiments.</p

Western blot of cell cycle and mTOR related proteins from lysates of UMUC-3, TCCSUP and RT112 cell lines.

<p>Tumor cells were pretreated with amygdalin for 24 h or 2 weeks (controls remained untreated). β-actin served as the internal control. One representative from three separate experiments is shown. The right panel of <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0105590#pone-0105590-g005" target="_blank">figure 5</a> shows pixel density values given in percentage related to controls not treated with amygdalin. *indicates significant difference to the control.</p

Adhesion of UMUC-3, TCCSUP and RT112 bladder cancer cells to HUVEC.

<p>Tumor cells were treated with 10 mg/ml amygdalin for either 24 h or for 2 weeks. Controls received cell culture medium alone. 0.5×10⁶ tumor cells/well were added to HUVEC monolayers for 0.5, 1 and 2 h. Mean adherent tumor cells from five fields was calculated and depicted as percentage of the 100% control (dotted line). One representative of six experiments is shown. *indicates significant difference to controls.</p

FACS analysis of integrin α and β subtype expression on TCCSUP cells.

<p>The left panel depicts integrin expression as histogram plots with a dotted line indicating background fluorescence and a solid line indicating specific fluorescence. The right panel shows integrin subtype expression after 24 h and 2 weeks amygdalin exposure, compared to controls set at 100%. n.c.  =  not calculated. * indicates significant difference to controls.</p

sj-docx-1-tau-10.1177_17562872241229248 – Supplemental material for Current role of intraoperative cell salvage techniques in the management of renal tumors with level III and IV inferior vena cava thrombus extension

Supplemental material, sj-docx-1-tau-10.1177_17562872241229248 for Current role of intraoperative cell salvage techniques in the management of renal tumors with level III and IV inferior vena cava thrombus extension by Cristian Surcel, Robert Dotzauer, Cristian Mirvald, Calin Popa, Cosmin Olariu, Catalin Baston, Mihai Harza, Constantin Gangu, Igor Tsaur and Ioanel Sinescu in Therapeutic Advances in Urology</p

FACS analysis of integrin α and β subtype expression on UMUC-3 cells.

<p>The left panel depicts integrin expression as histogram plots with a dotted line indicating background fluorescence and a solid line indicating specific fluorescence in untreated cells. The right panel shows integrin subtype expression after 24 h and 2 weeks amygdalin exposure, compared to controls set at 100%. n.c.  =  not calculated. * indicates significant difference to controls.</p

Intracellular integrin protein content of UMUC-3, TCCSUP and RT112 bladder cancer cells exposed to amygdalin for 24 h or 2 weeks.

<p>Controls remained untreated. β-actin served as the internal control. The figure shows one representative from three separate experiments. n.d. indicates “not detectable”. Quantification of integrin subtype expression is depicted on the right. Pixel density is given in percentage compared to controls not treated with amygdalin. *indicates significant increase after both 24 h and 2 weeks amygdalin treatment, #indicates significant decrease after both 24 h and 2 weeks amygdalin treatment, compared to controls.</p