Additional file 2: of Impact of insecticide-treated bednets and indoor residual spraying in controlling populations of Phlebotomus duboscqi, the vector of Leishmania major in Central Mali

Table S2. Meteorological information for the region of Segou, 2004 to 2017. (DOCX 19 kb

Additional file 1: of Impact of insecticide-treated bednets and indoor residual spraying in controlling populations of Phlebotomus duboscqi, the vector of Leishmania major in Central Mali

Table S1. Number of collected sand flies per household per month before and after IRS in the study area. Before IRS/LLINs, the number of collected flies per household ranged from 205 to 649 with a median of 293.00 and variance of 19778.94. After IRS/LLINs, the number of collected flies per household ranged from 25 to 159 with a median of 59.00 and variance of 3123.29. (DOCX 16 kb

<i>Leishmania</i> specific PCR products from field collected female <i>Phlebotomus duboscqi.</i>

<p><i>Leishmania</i> specific (A) and tubulin loading control (B) PCR products. N  =  negative control, P  =  positive control (DNA from <i>P. duboscqi</i> experimentally infected with <i>L. major</i>). L  =  100 bp ladder. Predicted band size of the PCR product for (A) <i>L. major</i> (650 bp) and (B) tubulin (325 bp) is indicated.</p

Collection success of female <i>Phlebotomus duboscqi</i> using various trapping methods.

<p>Number of female <i>P. duboscqi</i> collected per trapping method per night during monthly collection from July 2006 to June 2008 in two neighboring villages, Kemena (A) and Sougoula (B), in Central Mali: morning resting site collection (RSC AM)  =  female <i>P. duboscqi</i> (Pd) collected during 2 man hours, evening resting site collection (RSC PM)  =  female <i>P. duboscqi</i> collected during 2 man hours, tree light traps (TLT)  =  female <i>P.duboscqi</i> collected by 10 traps, indoor light traps (ILT)  =  female <i>P duboscqi</i> collected by 20 traps, outdoor light traps (OLT)  =  female <i>P. duboscqi</i> collected by 20 traps.</p

Estimated infection prevalence of <i>Leishmania major</i> in female <i>Phlebotomus duboscqi</i> from July 2006–June 2008.

<p>Estimated infection prevalence of <i>Leishmania major</i> in female <i>Phlebotomus duboscqi</i> from July 2006–June 2008.</p

Satellite image of study villages in Central Mali.

<p>Satellite image of the two study villages, Kemena (A) and Sougoula (B) illustrating the sites of monthly sand fly collections (circles) during the study of <i>Phlebotomus duboscqi</i> seasonality, July 2006 to June 2008. Red  =  compounds of indoor/outdoor house light trap collections; green  =  compounds of resting site collections; yellow  =  light trap location near trees. Image collected on May 22, 2006 by the Quickbird Satellite (DigitalGlobe, Inc. Longmont, CO USA).</p

Sand fly species diversity in two neighboring villages, Central Mali, March 2005-June 2006.

<p>Sand fly species diversity in two neighboring villages, Central Mali, March 2005-June 2006.</p

Estimated prevalence of infected sand flies collected from Kemena and Sougoula, two neighboring villages in Central Mali.

<p>Numbers in parenthesis represent the overall number of female <i>P. duboscqi</i> specimens tested in pools of 1–20 flies.</p

Monthly collections of <i>Phlebotomus duboscqi</i> by year in two neighboring villages in Central Mali.

<p>The combined total number of <i>P. duboscqi</i> (A) and female <i>P. duboscqi</i> (B) collected using two collection methods (light trap and resting site collection) during two collection nights per month per village over two consecutive years in the neighboring villages of Kemena and Sougoula in Central Mali.</p