Increase in efficiency of hydrogen production by optimization of food waste fermentation parameters

The aim of the work was to optimize the ratio of weight of solid (food waste) and liquid (water) phases in order to ensure high efficiency of molecular hydrogen synthesis and degradation of multi-component food waste. Assessment of the efficiency of fermentation process was carried out using colorimetric and potentiometric methods for pH and redox potential measuring, volumetric and chromatographic methods for volume and composition of gas investigation, and mathematical calculations for fermentation parameters determination. The dynamics of hydrogen fermentation of waste in the horizontal reactor using different ratios of solid (food waste) and liquid (water) phases was investigated. The optimization of the ratio of solid and liquid phases was shown to lead to the increase in efficiency of molecular hydrogen synthesis and destruction of waste particles. The ratio of solid and liquid phases 1:3 was determined to be optimal for the effective synthesis of hydrogen as well as for maximum waste decomposition. It provided effective hydrogen fermentation of multi-component food waste and allowed to rationally use material and technical resources. Obtained results are promising for further development of efficient industrial biotechnologies for waste destruction with the simultaneous synthesis of environmentally friendly energy carrier, i.e. molecular hydrogen

Identification of thermophilic anaerobic microorganisms isolated from the methane tank

Из термофильной накопительной метаногенной культуры, выделеной из активного ила метантенка станции биологической очистки сточных вод (СБО, Киев, Бортничи), были изолированы первичные анаэробы – термофильные целлюлолитические, сахаролитические штаммы и вторичные анаэробы – метанобразующие бактерии. Исследованы морфолого-культуральные и физиолого-биохимические свойства выделенных изолятов. На основании исследованных свойств выделенные штаммы идентифицированы как – Clostridium thermocellum 5СТ, C.thermosaccharolytіcum 1S, метаногенные бактерии отнесены к родам Methanobacterіum, Methanosarcіna.Primary anaerobes (thermophilic cellulolytic, saccharolytic strains) and secondary anaerobes (methane-forming bacteria) were isolated from the thermophilic accumulating methanogenic culture isolated from active silt of the methane tank at the station of biological purification of wastes (SBP, Kiev, Bortnichi). The morphological, cultural and physiological and biochemical properties isolates are studied. Proceeding from the studied properties, the isolated strains are identified as - Clostridium thermocellum 5CT, C.thermosaccharolytіcum 1S; methanogenic bacteria are attributed to genera Methanobacterіum, Methanosarcіna

Identification of thermophilic anaerobic microorganisms isolated from the methane tank

Из термофильной накопительной метаногенной культуры, выделеной из активного ила метантенка станции биологической очистки сточных вод (СБО, Киев, Бортничи), были изолированы первичные анаэробы – термофильные целлюлолитические, сахаролитические штаммы и вторичные анаэробы – метанобразующие бактерии. Исследованы морфолого-культуральные и физиолого-биохимические свойства выделенных изолятов. На основании исследованных свойств выделенные штаммы идентифицированы как – Clostridium thermocellum 5СТ, C.thermosaccharolytіcum 1S, метаногенные бактерии отнесены к родам Methanobacterіum, Methanosarcіna.Primary anaerobes (thermophilic cellulolytic, saccharolytic strains) and secondary anaerobes (methane-forming bacteria) were isolated from the thermophilic accumulating methanogenic culture isolated from active silt of the methane tank at the station of biological purification of wastes (SBP, Kiev, Bortnichi). The morphological, cultural and physiological and biochemical properties isolates are studied. Proceeding from the studied properties, the isolated strains are identified as - Clostridium thermocellum 5CT, C.thermosaccharolytіcum 1S; methanogenic bacteria are attributed to genera Methanobacterіum, Methanosarcіna

Increase in efficiency of hydrogen production by optimization of food waste fermentation parameters

The aim of the work was to optimize the ratio of weight of solid (food waste) and liquid (water) phases in order to ensure high efficiency of molecular hydrogen synthesis and degradation of multi-component food waste. Assessment of the efficiency of fermentation process was carried out using colorimetric and potentiometric methods for pH and redox potential measuring, volumetric and chromatographic methods for volume and composition of gas investigation, and mathematical calculations for fermentation parameters determination. The dynamics of hydrogen fermentation of waste in the horizontal reactor using different ratios of solid (food waste) and liquid (water) phases was investigated. The optimization of the ratio of solid and liquid phases was shown to lead to the increase in efficiency of molecular hydrogen synthesis and destruction of waste particles. The ratio of solid and liquid phases 1:3 was determined to be optimal for the effective synthesis of hydrogen as well as for maximum waste decomposition. It provided effective hydrogen fermentation of multi-component food waste and allowed to rationally use material and technical resources. Obtained results are promising for further development of efficient industrial biotechnologies for waste destruction with the simultaneous synthesis of environmentally friendly energy carrier, i.e. molecular hydrogen