DNA methylation analysis of regions (S3 Fig) of genes <i>AT3G01345</i>, <i>AT3G27473</i>, <i>AT3G30720</i>, <i>AT5G34850</i> in <i>MET1</i> transformants (+) and in lines derived from <i>MET1</i> transformants, from which the transgene has been removed (-).

<p>Lines A expresses a catalytically active <i>MET1</i> transgene, line I1 expresses a catalytically inactive <i>MET</i> transgene. Red bars denote CG methylation, blue bars CHG methylation and green bars CHH methylation.</p

Comparison of expression profiles of genes <i>AT3G01345</i>, <i>AT3G27473</i>, <i>AT3G30720</i>, <i>AT3G30820</i>, <i>AT4G25530 and AT5G34850</i> in MET1 lines.

<p>T3 seeds are labelled in blue, T4 seeds are labelled in orange.</p

Induction of epigenetic variation in Arabidopsis by over-expression of <i>DNA METHYLTRANSFERASE1 (MET1)</i> - Table

Induction of epigenetic variation in Arabidopsis by over-expression of <i>DNA METHYLTRANSFERASE1 (MET1)</i> - Tabl

RT-PCR analysis of four genes with dense methylation in MET1 transformants with (+) and without the transgene (-).

<p>Lines A1 and A2 express a catalytically active MET1 transgene, lines I1 and I2 express a catalytically inactive MET transgene. The mean and the standard error are shown for three biological replicates each tested in three technical replicates. Values on the y-axis represent the log2-fold-difference compared to the control line.</p

Shoot and root phenotypes in wildtype control plants, in MET1 transformants (+) and in lines derived from MET1 transformants, from which the transgene has been removed (-).

<p>Lines A1 and A2 express a catalytically active MET1 transgene, lines I1 and I2 express a catalytically inactive MET transgene. Images were taken eight weeks after stratification. The scale bar for shoot images indicates 5cm, the scale bar for root images indicates 10mm.</p

Comparison of expression profiles of genes <i>AT3G01345</i>, <i>AT3G27473</i>, <i>AT3G30720</i>, <i>AT3G30820</i>, <i>AT4G25530 and AT5G34850</i> in the <i>met1-1</i> mutant and <i>met1-1 RE</i>.

<p>The mean and the standard error are shown for three biological replicates each tested in three technical replicates. Values on the y-axis represent the fold-difference compared to the control line.</p

ChIP analysis of genes <i>At3G27473</i>, <i>At3G01345</i>, <i>At3G30720</i> and At5G34850 for H3K9me2, H3K4me3 and H4 acetylation marks.

<p>The means and the standard errors are shown for three biological replicates each tested in three technical replicates. Values on the y-axis represent the fold-difference of histone mark levels compared to the control line.</p

Reads pairs with discordant reads mapping to 7p15 and 7q21 within 200 bp of each other.

<p>Reads pairs with discordant reads mapping to 7p15 and 7q21 within 200 bp of each other.</p

Chromosome 7 ideogram and breakpoint confirmation.

<p><b>(A)</b> Arrows showing the breakpoint locations. Greek letters facilitate interpretation of the resulting pericentric inversion. Sanger sequencing results for the normal and breakpoint spanning amplicons for <b>(B)</b> the 7p15 and <b>(C)</b> the 7q21 inversion boundaries. The vertical dashed read line highlights the breakpoint. For ease of comparison a dashed black line has been drawn onto the normal sequence. (+): sense strand sequence; (-): antisense strand sequence. The inversion has resulted in an AT dinucleotide duplication which is shown arbitrarily assigned to the 7p15 breakpoint.</p