Продукция оксида азота мононуклеарами крови у больных лекарственно-чувствительным и лекарственно-устойчивым туберкулезом легких

Nitric oxide NO• production by blood monocytes was studied in 53 patients with drug-sensitive pulmonary tuberculosis (TB) and 50 patients with multidrug resistance (MDR) TB before and during the anti-tuberculosis therapy. Levels of NO• production were determined using the Griess's reactant. Increased levels of NO• secretion were observed before the treatment and after the intensive phase of therapy of drug-sensitive TB. However, it decreased after the full course of the anti-tuberculosis therapy. At the MDR TB the NO• production by monocytes was reduced before and after the intensive phase of treatment and increased after the end of the full course of therapy.Изучена продукция оксида азота NO• моноцитами периферической крови у 53 больных лекарственно-чувствительным и 50 пациентов с лекарственно-устойчивым туберкулезом легких (ТЛ) до, в процессе и после противотуберкулезной терапии. Уровень продукции NO• определяли с помощью реактива Грисса. При лекарственно-чувствительном ТЛ установлено повышение секреции NO• до начала терапии и после интенсивной фазы лечения и, напротив, ее угнетение после завершения полного курса противотуберкулезной терапии. У больных лекарственно-устойчивым ТЛ до и после проведения курса интенсивного лечения секреция NO• моноцитами крови снижалась и увеличивалась после окончания полного курса терапии

SOME FEATURES OF IMMUNE REGULATION IN PATIENTS WITH FIBROUS/CAVERNOUS PULMONARY TUBERCULOSIS

Abstract. The study dealt with subpopulation analysis of peripheral blood T-regulatory cells (Treg), and in  vitro  testing  of  immunosuppressive  cytokine  (IL-10,  TGF-β)  production  in  the  patients  with  fibrous/cavernous pulmonary tuberculosis, depending on results of intracutaneous tuberculin tests (Mantoux test). We  have  shown  that  Trn,  natural  T-regulatory  lymphocytes  (CD4+CD25+FoxP3+),  and  TGF-β  cytokine, produced by these cells, play a leading role for immune suppression developing in fibrosis-cavernous pulmonary tuberculosis. Moreover, an imbalance of Treg cell subsets has been revealed in patients with fibrous/ cavernous pulmonary tuberculosis, with either positive, or negative Mantoux reaction, as shown by increased content of Treg cells with CD4+CD25+FoxP3+ phenotype, along with higher amounts of CD4+CD25-FoxP3+ subpopulations, and deficiency of CD4+CD25+FoxP3- Treg lymphocytes in blood samples (in cases of positive Mantoux tests). Increased amounts of CD4+CD25+FoxP3+ Treg cells in peripheral blood of patients with fibrous/cavernous pulmonary  tuberculosis  is  associated  with  increase  in  basal  and  BCG-induced  TGF-β  production,  and decreased in vitro IL-10 secretory potential. (Med. Immunol., 2011, vol. 13, N 2-3, pp 267-272

The factors of dysregualtion of the immune response (at different stage of its implementation) in patients with pulmonary tuberculosis

The article provides an overview of scientific research to explain the mechanisms of inefficient implementation of antigen-specific immune response in patients with tuberculosis infection depending on the clinical form (infiltrative and disseminated pulmonary tuberculosis) and the course (drug-sensitive and drug-resistant pulmonary tuberculosis) of the disease. The mechanisms of immune imbalance in patients with pulmonary tuberculosis are associated with abnormal co-stimulation of a signal required for the activation of T-lymphocytes and immunosuppressive influence of regulatory T-cells both when they interact with dendritic cells at the inductive stage of the immune response, and in the process of differentiation and proliferation of effector cells with formation of suppressive mode of immune-regulation