1 research outputs found
Facile Solvothermal Preparation of Monodisperse Gold Nanoparticles and Their Engineered Assembly of FerritināGold Nanoclusters
Herein,
we report a quick and simple synthesis of water-soluble gold nanoparticles
using a HAuCl<sub>4</sub> and oleylamine mixture. Oleylamine serves
as a reduction agent as well as a stabilizer for nanoparticle surfaces.
The particle sizes can be adjusted by modulating reaction temperature
and time. Solvothermal reduction of HAuCl<sub>4</sub> with oleylamine
can be confirmed by measuring the product in Fourier transform infrared
(FTIR) spectroscopy. The plasmon band shifting from yellow to red
confirms a nanosized particle formation. Amide bonds on the surface
of the nanoparticles formed hydrogen bonds with one another, resulting
in a hydrophobic monolayer. Particles dispersed well in nonpolar organic
solvents, such as in hexane or toluene, by brief sonication. Next,
we demonstrated the transfer of gold nanoparticles into water by lipid
capsulation using 1-myristoyl-2-hydroxy-<i>sn</i>-glycero-3-phosphocholine
(MHPC), 1,2-distearoyl-<i>sn</i>-glycero-3-phosphoethanolamine-<i>N</i>-(methoxy polyethylene glycol)-2000 (DPPE-PEG2k), and 1,2-dioleoyl-<i>sn</i>-glycero-3-<i>N</i>-{5-amino-1-carboxypentyl}Āiminodiacetic
acid succinyl nickel salt [DGS-NTAĀ(Ni)]. The particle concentration
can be obtained using an absorbance in ultravioletāvisible
(UVāvis) spectra (at 420 nm). Instrumental analyses using transmission
electron microscopy (TEM), energy-dispersive X-ray (EDX) analysis,
dynamic light scattering (DLS), and FTIR confirmed successful production
of gold nanoparticles and fair solubility in water. Prepared gold
particles were selectively clustered via engineered ferritin nanocages
that provide multiple conjugation moieties. A total of 5ā6
gold nanoparticles were clustered on a single ferritin nanocage confirmed
in TEM. Reported solvothermal synthesis and preparation of gold nanoclusters
may serve as an efficient, alternate way of preparing water-soluble
gold nanoparticles, which can be used in a wide variety of biomedical
applications