Regenerasiprotoplas Tanaman Terung Dan Ketahanan Regeneran Terhadappenyakit Bakterilayu*[regeneration of Eggplant Protoplast and the Resistance to Bacterial Wilt]

Protoplasts regeneration was studied on 3 cultivars of eggplant (pupel, green, ang white fruits) and the regenerant were tested for their resistance to bacterial wilt. The researh was conducted in different steps, including protoplast isolation, protoplast purification, protoplast culture, cell suspention dilution, shoot regeneration, plantlet aclimatization, and testing for the resistant to bacterial wilt. Observations were made for protoplast number of each cultivar, protoplast cell wal formation, cell division, number of cell colony, microcalli, and shoot, percent of plantlet survival, in acclimatization, and the resistance of regenerants to bacterial wilt. Data was analized by calculating the average, percentage, and standart deviation. Results show that the combination of 0.5% cellulase Onozuka RS, 0.5% macerozyme R-10, 0.05% MES, and 9,1% mannitol in CPW solution was able to isolateds alot of sprotoplast (10 ) in each cultivars. The first culture incubation with out lighty in both (KM8P and VKM) media and reach which 0,2 mg/1 2,4-D, 0,5 mg/1 zeathin, 1 mg/1 IAA, promoted the formation of cell wall and cell division developed to microcalli. Regeneration media of MS, applied with vitamin MW, 0,1 mg/1 NAA, and 2 mg/1 zeathine produced 13 shoots.. From 21 regenerants acclimatized only 15 regenerants survive. There were differences among the regenerants in their resistance to bacterial wilt,3 regenerants resistant, 2 regenerant recovery (tolerant) and 10 regenerants were susceptibel

Radiosensitivitas dan Seleksi Mutan Putatif Jeruk Keprok Garut (Citrus Reticulata L.) Berdasarkan Penanda Morfologi

Mandarin Citrus cv. garut is a of local citrus variety with several superiority, but not competitive to imported citrus. Quality improvement have been the subject of citrus breeding programme. The objective of this research was to determine lethal dose 50 (LD50) and putative mutans regenerant selected based on morphological markers. Callus was irradiated at doses of 0, 20, 30, 40, 50, 60, 70, 80, 90 and 100 Gy and regenerated through somatic embryogenesis. The result of radiosensitivity showed that lethal dose 50 (LD50) analyzed by Curve Expert 1.4 software was 75.31 Gy. Observation on callus showed variation on morphology and weight of callus. Callus growth was not inhibited at doses 0-50 Gy but at doses 60-100 Gy callus growth was inhibited. Gamma irradiation also affected the formations of somatic embryos. After six weeks on maturation medium, the highest number of embryo somatic was produced from doses of 20 and 100 Gy and following culture on germination medium, the highest number of planlet was produced from doses of 20 and 40 Gy. Based on morphological characters, the selected regenerants had variability of 0-58%

Kualitas Kimia Susu Kambing Peranakan Etawa pada Berbagai Periode Laktasi di Desa Sungai Langka Kecamatan Gedong Tataan Kabupaten Pesawaran

This research was conducted from May up to June, 2016 at Ettawa grade goat (EGG) development area, Sungai Langka village, Gedong Tataan subdistrict, Pesawaran regency to study chemical quality of EGG milk. Survey method were used in this research. Fifteen tails of EGG at first, second, third, and fourth lactation period as sample research was decided by purposive sampling procedure. Variables observed were fat, protein, solid non fat, and water content. Chemical quality of milk were analysed at Technology of Agriculture Product Laboratory, Politeknik Negeri Lampung (Polinela), Bandarlampung. Chemical quality of the milk was compared Indonesia National Standard or Standar Nasional Indonesia (SNI) No 01-3141-1998 and Thai Agricultural Standard (TAS) 606-2008. Result of research indicated that fat content of 76.61 % samples (range of fat content 2.52 – 6.20 %) was qualified (standard of SNI was 3,7 %, TAS was 4.0 %). Protein content all samples (range 3.86 – 6.70 %) was qualified (standard of SNI was 2,7 %, TAS was 3.7 %). Solid non fat content all samples (range 9.54 – 11.92%) was qualified (standard of SNI was 8.0 %, TAS was 8.25). Water content all samples (range 76.28 – 82.48 %) was qualified (standard maximum of SNI was 89 %, standard maximum of TAS was 87 %).It could be concluded that chemical quality of EGG milk was qualified to standard of SNI No 01-3141-1998 and Thai Agricultural Standard (TAS) 606-2008

Perkecambahan Dan Perbanyakan Gaharu Secara in Vitro

Agarwood (Aquilaria malaccensis Lank) is one of the forest wood that are continously exploited. Currently, the Indonesian export of agarwood is decreasing because its population is endangered by excessive logging. Agarwood propagations need technology for reproduction of agarwood seedlings and their fungal inoculum. In vitro technique for germination of recalsitrant seeds and micropropagation are technologies that can be used for propagation of agarwood seedlings. An experiment was done to develop techniques for in vitro germination and micropropagation of agarwood. The in vitro germination was done using two different techniques. Firstly, sterile seeds were germinated on an MS medium + 50 mg/l PVP, 50 mg/l GA, and 1 mg/l BA or kinetin. Secondly, sterile seeds were germinated on basal medium of MS, 1/2 MS medium, MS medium without vitamins, as well as on MS medium without pyridoxine, nicotinic acid and WPM. Shoot initiations and multiplications were done on MS and 1/2 MS media containing 1, 3, or 5 mg/l BA. The explants used were cotyledone nodes, terminal shoots, single node with leaf, and sinle node without leaf. The results showed that the seed germination rate on the different media ranged from 7,14 to 50%. The seed germination rate on the MS medium without vitamis was the highest. The best explants for shoot induction and multiplication was single node with leaf which was cultured on MS + 1 mg/l BA

Peningkatan Toleransi Alumunium Pada Jeruk Batang Bawah Dengan Teknik Seleksi in Vitro Berulang

Aluminum Tolerance Improvement of Rootstock Citrusthrough Repeated In Vitro Selection. Mia Kosmiatin,Rosa Yunita, and Ali Husni. National orange productivitywas trend to decrease because of pathogen attack andreducing of planting area. One of alternative ways topreserve and increase orange productivity was usingmarginal soil mainly acid soil. This matter pushed thebreeder to prepare tolerant rootstock and stable in the acidsoil. In vitro culture technique was effective and efficientmethods to produce tolerant and stable rootstock in acid soilthrough simulation of acid soil with addition of highaluminum and low pH in the medium. By the simulation theselection could be done in cell level, so cell was selectedafter induction of variation. A rootstock which highcompatibility with scion, useful rooting, and aluminumtolerance could be increased orange productivity throughacid soil development. The research was conducted in 3phase: (1) induction of embryogenic calli, (2) improvementof genetic variation through mutation, and (3) In vitroselection with AlCl3.6H2O for aluminum and low pH tolerant.Immature embryos of rootstock were use as explant. Theresult showed that the best embryogenic calli were inducedon MS basal medium with MW vitamin + NAA 7,5 mg/l +kinetin 0,5 mg/l. Before selection, 1.000 rad dosage was themost tolerant dosage to growth embryogenic calli. Afterselection, 2.000 rad dosage was the best dosage to produceshoots which stable tolerant to aluminum. Selected 88mutant shoots were produced after three times selection onthe same medium which AlCl3.6H2O added at low pH