7 research outputs found
Glycogen synthase kinase 3α and 3β have distinct functions during cardiogenesis of zebrafish embryo-4
<p><b>Copyright information:</b></p><p>Taken from "Glycogen synthase kinase 3α and 3β have distinct functions during cardiogenesis of zebrafish embryo"</p><p>http://www.biomedcentral.com/1471-213X/7/93</p><p>BMC Developmental Biology 2007;7():93-93.</p><p>Published online 3 Aug 2007</p><p>PMCID:PMC1988812.</p><p></p>ybridization with staining revealed that randomized looping was observed in morphants (B-D). The expression of (F) and (H) appeared normal in morphants
Glycogen synthase kinase 3α and 3β have distinct functions during cardiogenesis of zebrafish embryo-8
<p><b>Copyright information:</b></p><p>Taken from "Glycogen synthase kinase 3α and 3β have distinct functions during cardiogenesis of zebrafish embryo"</p><p>http://www.biomedcentral.com/1471-213X/7/93</p><p>BMC Developmental Biology 2007;7():93-93.</p><p>Published online 3 Aug 2007</p><p>PMCID:PMC1988812.</p><p></p> to severe (B) defects in axis formation. At 72 hpf, both and morphants displayed pericardial edema (F, G, I, J) and an unlooped, stretched heart (I, J)
Glycogen synthase kinase 3α and 3β have distinct functions during cardiogenesis of zebrafish embryo-1
<p><b>Copyright information:</b></p><p>Taken from "Glycogen synthase kinase 3α and 3β have distinct functions during cardiogenesis of zebrafish embryo"</p><p>http://www.biomedcentral.com/1471-213X/7/93</p><p>BMC Developmental Biology 2007;7():93-93.</p><p>Published online 3 Aug 2007</p><p>PMCID:PMC1988812.</p><p></p> to severe (B) defects in axis formation. At 72 hpf, both and morphants displayed pericardial edema (F, G, I, J) and an unlooped, stretched heart (I, J)
Glycogen synthase kinase 3α and 3β have distinct functions during cardiogenesis of zebrafish embryo-2
<p><b>Copyright information:</b></p><p>Taken from "Glycogen synthase kinase 3α and 3β have distinct functions during cardiogenesis of zebrafish embryo"</p><p>http://www.biomedcentral.com/1471-213X/7/93</p><p>BMC Developmental Biology 2007;7():93-93.</p><p>Published online 3 Aug 2007</p><p>PMCID:PMC1988812.</p><p></p> of either -(-MO) or -mRNA (-MO), was injected into one-celled stage embryos and the heart morphology was observed at the stage as indicated. The elongation of heart tube was normally developed at 24 hpf in the wild-type (A) and in the morphants (C); whereas the heart of morphant did not elongate to from a heart-tube (B). The wild-type (D) and morphant's heart (F) developed normally at 30 hpf, but the heart of morphant was still retardant development at 30 hpf (E), and even ceased at heart-cone stage at 36 hpf (F). Compared to the wild-type (G), however, the heart positioning was abnormally in the morphant at 36 hpf (I, J). Eventually, both and morphants displayed an unlooped and stretched heart (L, M). The heart morphology of embryos injected with the control MO was also observed at 72 hpf (N). a: atrium; v: ventricle
Glycogen synthase kinase 3α and 3β have distinct functions during cardiogenesis of zebrafish embryo-5
<p><b>Copyright information:</b></p><p>Taken from "Glycogen synthase kinase 3α and 3β have distinct functions during cardiogenesis of zebrafish embryo"</p><p>http://www.biomedcentral.com/1471-213X/7/93</p><p>BMC Developmental Biology 2007;7():93-93.</p><p>Published online 3 Aug 2007</p><p>PMCID:PMC1988812.</p><p></p>d the left-predominant asymmetry persists through the stages of jogging (G). However, in morphants, the expression of becomes symmetrical at 20 hpf (B, D). In morphants, in which the heart fails to jog, is more evenly distributed in the heart region (H, I). The left-sided domain was greatly reduced in morphant hearts at 16 hpf (F). All are dorsal views. B, E are higher magnifications of A, D, respectively. Lines mark the midline. L, embryo left
Glycogen synthase kinase 3α and 3β have distinct functions during cardiogenesis of zebrafish embryo-7
<p><b>Copyright information:</b></p><p>Taken from "Glycogen synthase kinase 3α and 3β have distinct functions during cardiogenesis of zebrafish embryo"</p><p>http://www.biomedcentral.com/1471-213X/7/93</p><p>BMC Developmental Biology 2007;7():93-93.</p><p>Published online 3 Aug 2007</p><p>PMCID:PMC1988812.</p><p></p>analyzed by western blot. The antibody used is indicated in the left of each blot. Anti-GSK3 antibody enables to recognize both GSK3α and GSK3β proteins; anti-α-tubulin antibody was used as a loading control. The protein levels of GSK3α and GSK3β were reduced greatly in the protein lysates extracted from the – and -MO-injected embryos, respectively
Glycogen synthase kinase 3α and 3β have distinct functions during cardiogenesis of zebrafish embryo-6
<p><b>Copyright information:</b></p><p>Taken from "Glycogen synthase kinase 3α and 3β have distinct functions during cardiogenesis of zebrafish embryo"</p><p>http://www.biomedcentral.com/1471-213X/7/93</p><p>BMC Developmental Biology 2007;7():93-93.</p><p>Published online 3 Aug 2007</p><p>PMCID:PMC1988812.</p><p></p>phants. At 60–72 hpf,(A, B) and (C, D) expression was greatly up-regulated in morphants. Tg() embryos were injected with -MO and observed by two-photon fluorescence imaging of a live transgenic zebrafish heart at 100 hpf. The endocardial cells and blood are labeled yellow; the Hc-GFP-positive myocardial cells are labeled red. Valves are clearly observed in wild-type embryos (E; white arrows), but not in morphants (F). b, blood cells; V, ventricle; A, atrium