7 research outputs found
<i>Coccidioides</i> growth cycle and study overview.
<p><i>Coccidioides</i> growth cycle in culture (<b>A</b>), total RNA was collected at 96 hours from hyphae and spherules, which were grown at 30°C and 39°C, respectively. Lifecycle in culture illustration adapted from Delgado <i>et al</i>, 2003 <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0041034#pone.0041034-Delgado1" target="_blank">[11]</a>. Samples were collected in biological triplicate and the results from <i>C. immitis</i> isolate RS and <i>C. posadasii</i> isolate C735 were compared (<b>B</b>).</p
Differentially expressed genes in <i>C. immitis</i> and <i>C. posadasii</i>.
<p>Venn diagrams showing the number of genes commonly differentially regulated in the saprobic vs. parasitic growth phases of <i>C. immitis</i> and <i>C. posadasii</i>.</p
Top 15 genes with significantly higher expression (up-regulated) in the saprobic phase.
*<p>Fold difference is the mean saprobic/parasitic-phase expression level in <i>C. immitis</i> and <i>C. posadasii</i>.</p
Genes of interest from previous studies.
*<p>Expression ratio: parasitic/saprobic expression. Ratios >1.0 indicate higher expression (up-regulation) in the parasitic phase and ratios <1.0 indicate higher expression (up-regulation) in the saprobic phase.</p>**<p>p-value <0.05.</p
Top 15 genes with significantly higher expression (up-regulated) in the parasitic phase.
*<p>Fold difference is the mean parasitic/saprobic-phase expression level in <i>C. immitis</i> and <i>C. posadasii</i>.</p
mRNA expression levels of previously identified vaccine candidates [8].
*<p>Parasitic phase expression levels are normalized by library size and gene length.</p>**<p>Differential regulation: Up H: up-regulated in hyphae (saprobic phase), Up S: up-regulated in spherules (parasitic phase).</p
Median-difference boxplots.
<p>Median-difference boxplots showing the interquartile range (box with median line) of individual counts from the median count for all genes. Plot “whiskers” extend 1.5 times the interquartile range; outlier points are not shown. Ci: <i>C. immitis</i>, Cp: <i>C. posadasii</i>, H: hyphae (saprobic phase), S: spherule (parasitic phase).</p