Antimicrobial peptides and complement in neonatal hypoxia-ischemia induced brain damage

Hypoxic-ischemic encephalopathy (HIE) is a clinical condition in the neonate, resulting from oxygen deprivation around the time of birth. HIE affects 1-5/1000 live births worldwide and is associated with the development of neurological deficits, including cerebral palsy, epilepsy, and cognitive disabilities. Even though the brain is considered as an immune-privileged site, it has innate and adaptive immune response and can produce complement (C) components and antimicrobial peptides (AMPs). Dysregulation of cerebral expression of AMPs and C can exacerbate or ameliorate the inflammatory response within the brain. Brain ischemia triggers a prolonged inflammatory response affecting the progression of injury and secondary energy failure and involves both innate and adaptive immune systems, including immune-competent and non-competent cells. Following injury to the central nervous system (CNS), including neonatal hypoxia-ischemia (HI), resident microglia, and astroglia are the main cells providing immune defense to the brain in a stimulus-dependent manner. They can express and secrete pro-inflammatory cytokines and therefore trigger prolonged inflammation, resulting in neurodegeneration. Microglial cells express and release a wide range of inflammation-associated molecules including several components of the complement system. Complement activation following neonatal HI injury has been reported to contribute to neurodegeneration. Astrocytes can significantly affect the immune response of the CNS under pathological conditions through production and release of pro-inflammatory cytokines and immunomodulatory AMPs. Astrocytes express β-defensins, which can chemoattract and promote maturation of dendritic cells (DC), and can also limit inflammation by controlling the viability of these same DC. This review will focus on the balance of complement components and AMPs within the CNS following neonatal HI injury and the effect of that balance on the subsequent brain damage

Microbial Biosafety of Pilot-scale Bioreactor Treating MTBE and TBA-contaminated Drinking Water Supply

A pilot-scale sand-based fluidized bed bioreactor (FBBR) was utilized to treat both methyl tert-butyl ether (MTBE) and tert-butyl alcohol (TBA) from a contaminated aquifer. To evaluate the potential for re-use of the treated water, we tested for a panel of water quality indicator microorganisms and potential waterborne pathogens including total coliforms, Escherichia coli, Salmonella and Shigella spp., Campylobacter jejuni, Aeromonas hydrophila, Legionella pneumophila, Vibrio cholerae, Yersinia enterocolytica and Mycobacterium avium in both influent and treated waters from the bioreactor. Total bacteria decreased during FBBR treatment. E. coli, Salmonella and Shigella spp., C. jejuni, V. cholerae, Y. enterocolytica and M. avium were not detected in aquifer water or bioreactor treated water samples. For those pathogens detected, including total coliforms, L. pneumophila and A. hydrophila, numbers were usually lower in treated water than influent samples, suggesting removal during treatment. The detection of particular bacterial species reflected their presence or absence in the influent waters

А CONTRIBUTION ТО ТНЕ TECHNIQUE OF PREPARING ANATOMICAL SPECIMENS OF BONES, ARТICULATIONS AND SMALL SKELEТONS WIТH INTACT JOINTS

The bone, articulation and small skeleton preparations with intact joints аге of essential importance for thhe training of medical students, for establishment of anatomical museums as well as for research work. Three basic stages аге distinguished in the working technique  of such preparations, namely: maceration of the soft tissues, defatting and bleaching. The stage of greatest importance is the maceration. Most of the methods of maceration аге biologically conditioned, i. е. based оn the natural property of the tissues to putrefy. In warm water the maceration ocours in one ог two months. With skilfull  regulation of temperature this process could bе completed in 6-10 days (Mechanic - 1935, Yaroslavtzev - 1961). The speediпg up of the process is obtained bу means of adding Bact. proteus culture (Sorokiп - 1942) оr bу pouring the mateгial with uric acid (Bordas - 1928, Dyakonov - 1935). Chemical methods of maceration also exist, based on the ргорегtу of certan chemical substances to destroy organic matter. With this purpose potassium hydroxide solution was employed (Petrov - 1896, Ivanov - 1946). Antiformin is also used, received bу mixing chlorosis аnd sodium hydroxide during which reaction oxygen is freed, responsibe for the destruction of organic substances. Andreev (1955) reports good results with the application of this method. Defatting of bоnеs is obtained bу various methods: boiling in sodium hydroxide solution, immersion in petrole, weak solution of sodium peroxide etc. For thebleaching of bones solutions аrе used calcium hydroxide, hydrogen peroxide, sodium peroxide and others

Thermoacoustic tomography with variable sound speed

We study the mathematical model of thermoacoustic tomography in media with a variable speed for a fixed time interval, greater than the diameter of the domain. In case of measurements on the whole boundary, we give an explicit solution in terms of a Neumann series expansion. We give necessary and sufficient conditions for uniqueness and stability when the measurements are taken on a part of the boundary

Effect of Benzene and Ethylbenzene on the Transcription of methyl-\u3cem\u3etert\u3c/em\u3e-butyl Ether Degradation Genes of \u3cem\u3eMethylibium petroleiphilum\u3c/em\u3e PM1

Methyl-tert-butyl ether (MTBE) and its degradation by-product, tert-butyl alcohol (TBA), are widespread contaminants detected frequently in groundwater in California. Since MTBE was used as a fuel oxygenate for almost two decades, leaking underground fuel storage tanks are an important source of contamination. Gasoline components such as BTEX (benzene, toluene, ethylbenzene and xylenes) are often present in mixtures with MTBE and TBA. Investigations of interactions between BTEX and MTBE degradation have not yielded consistent trends, and the molecular mechanisms of BTEX compounds’ impact on MTBE degradation are not well understood. We investigated trends in transcription of biodegradation genes in the MTBE-degrading bacterium, Methylibium petroleiphilum PM1 upon exposure to MTBE, TBA, ethylbenzene and benzene as individual compounds or in mixtures. We designed real-time quantitative PCR assays to target functional genes of strain PM1 and provide evidence for induction of genes mdpA (MTBE monooxygenase), mdpJ (TBA hydroxylase) and bmoA (benzene monooxygenase) in response to MTBE, TBA and benzene, respectively. Delayed induction of mdpA and mdpJ transcription occurred with mixtures of benzene and MTBE or TBA, respectively. bmoA transcription was similar in the presence of MTBE or TBA with benzene as in their absence. Our results also indicate that ethylbenzene, previously proposed as an inhibitor of MTBE degradation in some bacteria, inhibits transcription of mdpA, mdpJ and bmoAgenes in strain PM1

Gene \u3cem\u3emdpC\u3c/em\u3e Plays a Regulatory Role in the Methyl-\u3cem\u3etert\u3c/em\u3e-butyl Ether Degradation Pathway of \u3cem\u3eMethylibium petroleiphilum\u3c/em\u3e Strain PM1

Among the few bacteria known to utilize methyl tert-butyl ether (MTBE) as a sole carbon source, Methylibium petroleiphilum PM1 is a well-characterized organism with a sequenced genome; however, knowledge of the genetic regulation of its MTBE degradation pathway is limited. We investigated the role of a putative transcriptional activator gene, mdpC, in the induction of MTBE-degradation genes mdpA (encoding MTBE monooxygenase) and mdpJ (encoding tert-butyl alcohol hydroxylase) of strain PM1 in a gene-knockout mutant mdpC−. We also utilized quantitative reverse transcriptase PCR assays targeting genes mdpA, mdpJ and mdpC to determine the effects of the mutation on transcription of these genes. Our results indicate that gene mdpC is involved in the induction of both mdpA and mdpJ in response to MTBE and tert-butyl alcohol (TBA) exposure in PM1. An additional independent mechanism may be involved in the induction of mdpJ in the presence of TBA

Temporal Analysis of Activity Patterns of Editors in Collaborative Mapping Project of OpenStreetMap

In the recent years Wikis have become an attractive platform for social studies of the human behaviour. Containing millions records of edits across the globe, collaborative systems such as Wikipedia have allowed researchers to gain a better understanding of editors participation and their activity patterns. However, contributions made to Geo-wikis_wiki-based collaborative mapping projects_ differ from systems such as Wikipedia in a fundamental way due to spatial dimension of the content that limits the contributors to a set of those who posses local knowledge about a specific area and therefore cross-platform studies and comparisons are required to build a comprehensive image of online open collaboration phenomena. In this work, we study the temporal behavioural pattern of OpenStreetMap editors, a successful example of geo-wiki, for two European capital cities. We categorise different type of temporal patterns and report on the historical trend within a period of 7 years of the project age. We also draw a comparison with the previously observed editing activity patterns of Wikipedia.Comment: Submitte

To Duckweeds (\u3cem\u3eLandoltia punctata\u3c/em\u3e), Nanoparticulate Copper Oxide is More Inhibitory than the Soluble Copper in the Bulk Solution

CuO nanoparticles (CuO-NP) were synthesized in a hydrogen diffusion flame. Particle size and morphology were characterized using scanning mobility particle sizing, Brunauer–Emmett–Teller analysis, dynamic light scattering, and transmission electron microscopy. The solubility of CuO-NP varied with both pH and presence of other ions. CuO-NP and comparable doses of soluble Cu were applied to duckweeds, Landoltia punctata. Growth was inhibited 50% by either 0.6 mg L−1 soluble copper or by 1.0 mg L−1 CuO-NP that released only 0.16 mg L−1 soluble Cu into growth medium. A significant decrease of chlorophyll was observed in plants stressed by 1.0 mg L−1 CuO-NP, but not in the comparable 0.2 mg L−1 soluble Cu treatment. The Cu content of fronds exposed to CuO-NP is four times higher than in fronds exposed to an equivalent dose of soluble copper, and this is enough to explain the inhibitory effects on growth and chlorophyll content

Plasticity in the Neonatal Brain following Hypoxic-Ischaemic Injury

Hypoxic-ischaemic damage to the developing brain is a leading cause of child death, with high mortality and morbidity, including cerebral palsy, epilepsy, and cognitive disabilities. The developmental stage of the brain and the severity of the insult influence the selective regional vulnerability and the subsequent clinical manifestations. The increased susceptibility to hypoxia-ischaemia (HI) of periventricular white matter in preterm infants predisposes the immature brain to motor, cognitive, and sensory deficits, with cognitive impairment associated with earlier gestational age. In term infants HI causes selective damage to sensorimotor cortex, basal ganglia, thalamus, and brain stem. Even though the immature brain is more malleable to external stimuli compared to the adult one, a hypoxic-ischaemic event to the neonate interrupts the shaping of central motor pathways and can affect normal developmental plasticity through altering neurotransmission, changes in cellular signalling, neural connectivity and function, wrong targeted innervation, and interruption of developmental apoptosis. Models of neonatal HI demonstrate three morphologically different types of cell death, that is, apoptosis, necrosis, and autophagy, which crosstalk and can exist as a continuum in the same cell. In the present review we discuss the mechanisms of HI injury to the immature brain and the way they affect plasticity