32 research outputs found
Electrosynthesized molecularly imprinted polymers for protein recognition
Molecularly imprinted polymers (MIPs) for the recognition of proteins are expected to possess high affinity through the establishment of multiple interactions between the polymer matrix and the large number of functional groups of the target. However, while highly affine recognition sites need building blocks rich in complementary functionalities to their target, such units are likely to generate high levels of non-specific binding. This paradox, that nature solved by evolution for biological receptors, needs to be addressed by the implementation of new concepts in molecular imprinting of proteins. Additionally, the structural variability, large size and incompatibility with a range of monomers made the development of protein MIPs to take a slow start. While the majority of MIP preparation methods are variants of chemical polymerization, the polymerization of electroactive functional monomers emerged as a particularly advantageous approach for chemical sensing application. Electropolymerization can be performed from aqueous solutions to preserve the natural conformation of the protein templates, with high spatial resolution and electrochemical control of the polymerization process. This review compiles the latest results, identifying major trends and providing an outlook on the perspectives of electrosynthesised protein-imprinted MIPs for chemical sensing
Environmental and Toxicological Impacts of Glyphosate with Its Formulating Adjuvant
Environmental and toxicological characteristics of formulated pesticides may substantially differ from those of their active ingredients or other components alone. This phenomenon is demonstrated in the case of the herbicide active ingredient glyphosate. Due to its extensive application, this active ingredient was found in surface and ground water samples collected in Békés Country, Hungary, in the concentration range of 0.54–0.98 ng/ml. The occurrence of glyphosate appeared to be somewhat higher at areas under intensive agriculture, industrial activities and public road services, but the compound was detected at areas under organic (ecological) farming or natural grasslands, indicating environmental mobility. Increased toxicity of the formulated herbicide product Roundup compared to that of glyphosate was observed on the indicator aquatic organism Daphnia magna Straus. Acute LC50 values of Roundup and its formulating adjuvant polyethoxylated tallowamine (POEA) exceeded 20 and 3.1 mg/ml, respectively, while that of glyphosate (as isopropyl salt) was found to be substantially lower (690-900 mg/ml) showing good agreement with literature data. Cytotoxicity of Roundup, POEA and glyphosate has been determined on the neuroectodermal cell line, NE-4C measured both by cell viability test and holographic microscopy. Acute toxicity (LC50) of Roundup, POEA and glyphosate on NE-4C cells was found to be 0.013±0.002%, 0.017±0.009% and 6.46±2.25%, respectively (in equivalents of diluted Roundup solution), corresponding to 0.022±0.003 and 53.1±18.5 mg/ml for POEA and glyphosate, respectively, indicating no statistical difference between Roundup and POEA and 2.5 orders of magnitude difference between these and glyphosate. The same order of cellular toxicity seen in average cell area has been indicated under quantitative cell visualization. The results indicate that toxicity of the formulated herbicide is caused by the formulating agent, but in some parameters toxicological synergy occurs between POEA and glyphosate
Objective and Subjective Components of the First-Night Effect in Young Nightmare Sufferers and Healthy Participants
The first-night effect—marked differences between the first- and the second-night sleep spent in a
laboratory—is a widely known phenomenon that accounts for the common practice of excluding
the first-night sleep from any polysomnographic analysis. The extent to which the first-night effect
is present in a participant, as well as its duration (1 or more nights), might have diagnostic value
and should account for different protocols used for distinct patient groups. This study investigated
the first-night effect on nightmare sufferers (NM; N D 12) and healthy controls .N D 15/ using
both objective (2-night-long polysomnography) and subjective (Groningen Sleep Quality Scale
for the 2 nights spent in the laboratory and 1 regular night spent at home) methods. Differences
were found in both the objective (sleep efficiency, wakefulness after sleep onset, sleep latency,
Stage-1 duration, Stage-2 duration, slow-wave sleep duration, and REM duration) and subjective
(self-rating) variables between the 2 nights and the 2 groups, with a more pronounced first-night
effect in the case of the NM group. Furthermore, subjective sleep quality was strongly related to
polysomnographic variables and did not differ among 1 regular night spent at home and the second
night spent in the laboratory. The importance of these results is discussed from a diagnostic point
of view