A Point-Source Method for Rapid Simultaneous Estimation of Soil Hydraulic and Chemical Transport Properties

Hydraulic and chemical transport properties are needed for accurate prediction of water and chemical movement through the vadose zone. Field methods used to estimate such properties are often hampered by extensive labor and time constraints. One of the objectives of this study was to develop an experimental setup and a procedure for a point-source method that facilitates rapid and simultaneous measurements of soil hydraulic and chemical transport properties at multiple locations. Another objective was to evaluate the point-source method by comparing the parameters with those produced by ponded and tension infiltrometers. The experimental setup consisted of three dripper lines equipped with pressure-compensating drippers. The setup was evaluated on a greenhouse soil pit. Determined hydraulic properties were the saturated hydraulic conductivity (K s) and the macroscopic capillary length (λc). Hydraulic properties (from the point-source method) were determined by applying four consecutive discharge rates on the soil surface and measuring their corresponding steady-state saturated areas. Determined chemical transport parameters were the immobile water fraction (θim/θ) and the mass exchange coefficient (α). They were determined by applying a sequence of conservative fluorobenzoate tracers. The point-source method gave consistent and reliable estimates for both sets of properties. Except for α, there was no significant difference between the two procedures (point source vs. infiltrometers) in determining both sets of properties. The study showed that the point-source setup could be utilized for rapid and simultaneous estimation of soil hydraulic and chemical transport properties at multiple locations with minimum labor requirements

Field determination of soil hydraulic and chemical transport properties

Hydraulic and chemical transport properties are the major inputs in predictive models that simulate the movement of water and chemicals through the vadose zone. However, there is a lack of field measurements of such properties to verify models describing water and chemical movement through the soil. One of the objectives of this study was to use a point source method to determine simultaneously the hydraulic and chemical transport properties at multiple field locations. A second objective was to determine the spatial distribution of such properties across a field. A total of 50 field locations within a 7 × 15-m area were rapidly and simultaneously evaluated for such properties. The hydraulic properties were the saturated hydraulic conductivity (Ks) and the macroscopic capillary length (λc). The chemical transport properties were the immobile water content, expressed as a fraction of water content (θim/θ) and the mass exchange coefficient (α). The hydraulic properties were determined by applying three discharge rates from irrigation dripper lines and measuring the resultant steady-state flux densities at the soil surface beneath each emitter. The chemical transport properties were determined by applying a sequence of three conservative tracers at a steady-state infiltration rate and measuring their resident concentration in the soil. The Ks values ranged from 7.5 to 79.0 cm h−1, with a median of 27.4 cm h−1 (± 16.8). The λc values ranged from 0.03 to 13.1 cm, with a median of 2.6 cm (± 3.6). The θim/θ values ranged from 0.36 to 0.88, with a median of 0.57 (± 0.098). The α values ranged from 0.002 to 0.12 h−1, with a median of 0.034 h−1 (± 0.027). The values of the hydraulic and chemical transport parameters were found to be comparable with values reported by studies conducted on nearby field locations on similar soil. Based on semivariogram analysis, the measured properties were not spatially correlated. Because the method required only 2 days to collect data it should prove useful for future studies that require extensive field measurements of hydraulic and chemical transport properties

Gut microbiome is associated with multiple sclerosis activity in children

OBJECTIVE: To identify features of the gut microbiome associated with multiple sclerosis activity over time. METHODS: We used 16S ribosomal RNA sequencing from stool of 55 recently diagnosed pediatric-onset multiple sclerosis patients. Microbiome features included the abundance of individual microbes and networks identified from weighted genetic correlation network analyses. Prentice-Williams-Peterson Cox proportional hazards models estimated the associations between features and three disease activity outcomes: clinical relapses and both new/enlarging T2 lesions and new gadolinium-enhancing lesions on brain MRI. Analyses were adjusted for age, sex, and disease-modifying therapies. RESULTS: Participants were followed, on average, 2.1 years. Five microbes were nominally associated with all three disease activity outcomes after multiple testing correction. These included butyrate producers Odoribacter (relapse hazard ratio = 0.46, 95% confidence interval: 0.24, 0.88) and Butyricicoccus (relapse hazard ratio = 0.49, 95% confidence interval: 0.28, 0.88). Two networks of co-occurring gut microbes were significantly associated with a higher hazard of both MRI outcomes (gadolinium-enhancing lesion hazard ratios (95% confidence intervals) for Modules 32 and 33 were 1.29 (1.08, 1.54) and 1.42 (1.18, 1.71), respectively; T2 lesion hazard ratios (95% confidence intervals) for Modules 32 and 33 were 1.34 (1.15, 1.56) and 1.41 (1.21, 1.64), respectively). Metagenomic predictions of these networks demonstrated enrichment for amino acid biosynthesis pathways. INTERPRETATION: Both individual and networks of gut microbes were associated with longitudinal multiple sclerosis activity. Known functions and metagenomic predictions of these microbes suggest the important role of butyrate and amino acid biosynthesis pathways. This provides strong support for future development of personalized microbiome interventions to modify multiple sclerosis disease activity

A dripper-TDR method for in situ determination of hydraulic conductivity and chemical transport properties of surface soils

Field determined hydraulic and chemical transport properties can be useful for the protection of groundwater resources from land-applied chemicals. Most field methods to determine flow and transport parameters are either time or energy consuming and/or they provide a single measurement for a given time period. In this study, we present a dripper-TDR field method that allows measurement of hydraulic conductivity and chemical transport parameters at multiple field locations within a short time period. Specifically, the dripper-TDR determines saturated hydraulic conductivity (Ks), macroscopic capillary length (λc), immobile water fraction (θim/θ), mass exchange coefficient (α) and dispersion coefficient (Dm). Multiple dripper lines were positioned over five crop rows in a field. Background and step solutions were applied through drippers to determine surface hydraulic conductivity parameters at 44 locations and surface transport properties at 38 locations. The hydraulic conductivity parameters (Ks, λc) were determined by application of three discharge rates from the drippers and measurements of the resultant steady-state flux densities at the soil surface beneath each dripper. Time domain reflectometry (TDR) was used to measure the bulk electrical conductivity of the soil during steady infiltration of a salt solution. Breakthrough curves (BTCs) for all sites were determined from the TDR measurements. The Ks and λcvalues were found to be lognormally distributed with average values of 31.4 cm h−1 and 6.0 cm, respectively. BTC analysis produced chemical properties, θim/θ, α, and Dm with average values of 0.23, 0.0036 h−1, and 1220 cm2 h−1, respectively. The estimated values of the flow and transport parameters were found to be within the ranges of values reported by previous studies conducted at nearby field locations. The dripper TDR method is a rapid and useful technique for in situ measurements of hydraulic conductivity and solute transport properties. The measurements reported in this study give clear evidence to the occurrence of non-equilibrium water and chemical movement in surface soil. The method allows for quantification of non-equilibrium model parameters and preferential flow. Quantifying the parameters is a necessary step toward determining the influences of surface properties on infiltration, runoff, and vadose zone transport

The design and development of a multicentric protocol to investigate the impact of adjunctive doxycycline on the management of peripheral lymphoedema caused by lymphatic filariasis and podoconiosis

BACKGROUND: As new lymphatic filariasis infections are eliminated through mass chemotherapy, previously affected individuals are left with the sequellae, especially chronic progressive lymphoedema. Currently this is managed by careful attention to limb hygiene to prevent infection. Studies over the past 15 years have suggested that the incorporation of doxycycline treatment may arrest or even reverse progression of lymphoedema. Most of this work has been observational or based on small studies, and if this intervention is effective, studies need to be conducted on a larger scale and under diverse geographical and social conditions before it can be incorporated into treatment policy. METHODS/DESIGN: The double-blind, placebo-controlled study was designed to investigate the impact of six weeks treatment with doxycycline added to standard limb hygiene on early stage filarial lymphoedema in five sites in Africa and the Indian subcontinent. One site in Cameroon is selected for studying lymphoedema in podoconiosis. Each site was individually powered with the potential to undertake a meta-analysis on completion. Evaluation methods followed those used in Ghana in 2012 with additions resulting from advances in technology. The details of the core protocol and how it was varied to take account of differing situations at each of the sites are provided. The study will enrol up to 1800 patients and will complete in mid-2021. CONCLUSIONS: This paper provides details of what challenges were faced during its development and discusses the issues and how they were resolved. In particular, the reasons for inclusion of new technology and the problems encountered with the supply of drugs for the studies are described in detail. By making these details available, it is hoped that the study protocol will help others interested in improving treatment for filarial lymphoedema in the design of future studies. Trial registration India: Clintrials.gov. NCT02929121 registered 10 Oct 2016: https://clinicaltrials.gov/ct2/show/NCT02929121 Mali: Clintrials.gov. NCT02927496 registered 7 Oct 2016: https://clinicaltrials.gov/ct2/show/NCT0292749 Sri Lanka: Clintrials.gov. NCT02929134 registered 10 Oct 2016: https://clinicaltrials.gov/ct2/show/NCT02929134 Ghana: ISRCTN. 14042737 registered 10 July 2017: https://doi.org/10.1186/ISRCTN14042737 Tanzania: ISRCTN. 65756724 registered 21 July 2017: https://doi.org/10.1186/ISRCTN65756724 Cameroon: ISRCTN. 1181662 registered 25 July 2017: https://doi.org/10.1186/ISRCTN11881662

The dissolution and solid-state behaviours of coground ibuprofen–glucosamine HCl

The cogrinding technique is one of most effective methods for improving the dissolution of poorly water-soluble drugs and it is superior to other approaches from an economical as well as an environmental standpoint, as the technique does not require any toxic organic solvents. Present work explores the role of d-glucosamine HCl (GL) as a potential excipient to improve dissolution of a low melting point drug, ibuprofen (Ibu), using physical mixtures and coground formulations. The dissolution of the poorly soluble drug has been improved by changing the ratio of Ibu:GL and also grinding time. The results also showed that although GL can enhance the solubility of Ibu, it also reduces pH around the Ibu particles which led to poor dissolution performance when the concentration of GL is high. The effect of GL on the solubility of Ibu could be misleading if the pH of the final solution was not measured. Grinding reduced the particle size of GL significantly but in case of Ibu it was less effective. Solid state analysis (XRPD, DSC, and FT-IR) showed that ibuprofen is stable under grinding conditions, but the presence of high concentration of GL in samples subjected to high grinding times caused changes in FT-IR spectrum of Ibu which could be due to intermolecular hydrogen bond or esterification between the carboxylic acid group in the ibuprofen and hydroxyl group in the GL