白血球，単核球，顆粒球，T-リンパ球，非Tリンパ球，単球中の亜鉛値について

This study was designed to evaluate the zinc concentrations of leukocytes: monon- uclear cells, granulocytes, T and non-T lymphocytes and monocytes.　　The zinc content of leukocytes was 0.137 ±0.040 μg/10⁷ cells. The zinc content of mononuclear cells (0.188±0.030 μg/10⁷ cells) was higher than in granulocytes (0.086± 0.023 μg/10⁷ cells) (p<0.001). The mononuclear cell-granulocyte ratio for zinc was approximately 2 : 1. There was no statistically significant difference between the zinc content of T lymphocytes and non-T lymphocytes. The zinc content of monocytes (0.337 ±0.026 μg/10⁷ cells) was highest in any other type of leukocyte (p<0.001).　　Zinc in leukocytes may, in part, influence immunity since zinc is an important part of the structure of many vital enzymes, including DNA polymerase, DNA dependent RNA polymerase and thymidine kinase, and plays a central role in cellular metabolism

リンパ球活性化におけるコンカナバリンAの接触時間に対するノイラミニダーゼの作用

Neuraminidase-treatment greatly increased 3H-thymidine incorporation by human lymphocytes at lower concentrations of Con A. An irreversible sequence after 24 hr of incubation in the presence of the optimal concentration of Con A was found. However, at a suboptimal concantraiton of Con A, neuraminidase-treated lymphocytes exposed to Con A for 72 hr exhibited a greater magnitude of 3H-thymidine incorporation in contrast to the neuraminidase-treated lymphocytes exposed to Con A for 24 hr, indicating that the enancement effect of neuraminidase was not due to the increased binding of Con A to cell surfaces.This work was supported in part by Grant-in-Aid for Scientific Research (Project Nos. 544050 and 577401) from the Ministry of Education, Science and Culture of Japan