Behavior of some winter wheat varieties and perspective lines at A.R.D.S. Lovrin

The present paper aims to analyze the behavior of twenty-five Romanian wheat varieties and perspective lines, in the process of homologation, in terms of the yields of the grains obtained in the agricultural year 2016-2017 at SCDA Lovrin.The climatic year 2016-2017 was an excellent year due to the fact that sowing was done late in autumn, and emergence of the plants occurred in the spring, which determined the specific reactions of the wheat varieties, expressed in the productions as well as in morphological characters.Variance analysis revealed differences between the twentyfive varieties studied, with climatic conditions in this period having significant effects on the behavior of studied wheat varieties.The average production of the studied genotypes ranged between 7917 kg/ha at the Viteaz prospecting wheat variety, and 4958 kg/ha 11424G-1 the winter wheat prospecting variety, and the coefficient of variation had the lowest values for perspective winter wheat Pitar, Pajura, Signal and 11424G-1

The Effect Citrox BCL on Legionella pneumophila Mechanisms of Biofilm Formation, Oxidative Stress and Virulence

Publication history: Accepted - 1 November 2022; Published online - 4 November 2022Legionella pneumophila is responsible for causing Legionnaires’ disease and Pontiac fever, also known as legionellosis. The aim of this study was to investigate the mechanistic effect of a mixture of natural antimicrobials (Citrox BCL) in preventing L. pneumophila biofilm formation and reducing its in vitro virulence. The minimum inhibitory concentrations were detected at 0.06%, and the MBC was established at 0.125%. Based on the growth curve profile, the sub-inhibitory concentration of 0.02% was further used to study the mechanistic implications in the absence of a cytotoxic effect on A549 cells. At 24 h post-infection, Citrox BCL reduced (p = 0.005) the intracellular growth of L. pneumophila when the A549 cells or the bacteria were pre-treated with 0.02% Citrox BCL. This result was replicated when Citrox BCL was added during the 24 h infection assay leading to a reduction in intracellular growth (p = 0.003). Herein we show that at the sub-inhibitory concentration of 0.02%, Citrox CBL lowers the ROS levels in infected A549 cells and causes a 45% reduction in L. pneumophila EPS production, a reduction associated with the decline in biofilm formation. Overall, our results corroborate the low c-di-GMP production with the decrease in biofilm formation and low EPS levels. The low EPS levels seemed to be caused by the downregulation of the tatB and tatC gene expressions. Moreover, inhibition of pvcA and pvcB gene expressions, leading to lower siderophore levels, suggests that Citrox BCL reduces the ability of L. pneumophila to sequester iron and reduce biofilm formation through iron starvation.We acknowledge Environtech, Dublin, Ireland, for funding the Ph.D. of Eugenia Butucel. Grant number 49650

Influence of Sample Preparation/Extraction Method on the Phytochemical Profile and Antimicrobial Activities of 12 Commonly Consumed Medicinal Plants in Romania

This paper aims to evaluate the influence of preparation and extraction methods on the phytochemical profile and microbiological activity of 12 medicinal plants from the west side of Romania. First, the proximate composition (humidity, proteins, lipids, and ash) and elemental composition of the raw material were evaluated. Two levels of plant shredding were used: coarse shredding (CS) and fine grinding of plants to obtain powder (FG), and three extraction methods: conventional solvent extraction (CES), ultrasound-assisted extraction (UAE), and microwave extraction (MWE). The phytochemical profile investigated referred to antioxidant activity (AA), total polyphenol content (TPC), and flavonoid content (TFC), using spectrophotometric methods, and individual polyphenols detected using the LC/MS method. The preparation/extraction method for each medicinal plant was optimized using statistical analysis. The optimized extracts for each medicinal plant were tested to evaluate the antimicrobial potential against 9 standard strains. The results showed that the sample preparation method before extraction (shredding or grinding) influences the content of phytocompounds by increasing them in powder form. The use of green technologies, especially MWE, leads to the highest content of TPC, TFC, and AA. The TPC value ranged between 4.83–19.2 mgGAE/g DM in the samples CS and between 19.00–52.85 mgGAE/g DM in the samples FG. The highest antioxidant value was found in the Origanum vulgare extract, both in the case of the crushed sample (22.66 mM Fe2+/100 g) and the powder sample (81.36 mM Fe2+/100 g), followed by Melissa officinalis, The TFC values varied in the range of 1.02–3.46 mgQE/g DM when CES was used, between 2.36–12.09 mgQE/g DM for UAE, and between 1.22–9.63 mgQE/g DM in the case of the MWE procedure. The antimicrobial activity highlighted the effectiveness of the extracts, especially on the strains of H. influenzae, C. albicans, C. parapsilosis, S. aureus, and S. flexneri. Reduced antimicrobial activity was recorded for the strains of S. pyogenes and P. aeruginosa. The best antimicrobial activity was registered by Thymus serpyllum, with an inhibition rate of 132.93% against E. coli and 78.40% against C. albicans