4 research outputs found
Probing DNA-stabilized fluorescent silver nanocluster spectral heterogeneity by time-correlated single photon counting
Direct observation of multiple conformational states in Cytochrome P450 oxidoreductase and their modulation by membrane environment and ionic strength
Probing the Absorption and Emission Transition Dipole Moment of DNA Stabilized Silver Nanoclusters
Using single molecule
polarization measurements, we investigate
the excitation and emission polarization characteristics of DNA stabilized
silver nanoclusters (C24-AgNCs). Although small changes in the polarization
generally accompany changes to the emission spectrum, the emission
and excitation transition dipoles tend to be steady over time and
aligned in a similar direction, when immobilized in PVA. The emission
transition dipole patterns, observed for C24-AgNCs in defocused wide
field imaging, match that of a single emitter. The small changes to
the polarization and spectral shifting that were observed could be
due to changes to the conformation of the AgNC or the DNA scaffold.
Although less likely, an alternative explanation could be that several
well aligned spectrally similar emitters are present within the DNA
scaffold which, due to Förster resonance energy transfer (FRET)
processes such as energy hopping, energy transfer, and singlet–singlet
annihilation, behave as a single emitter. The reported results can
provide more insight in the structural and photophysical properties
of DNA-stabilized AgNCs