The identification of electrical mark by the FTIR-based PLS model in an independent tissue section.

<p>HE stained skin tissue sections are presented here where elongation cell in electrical mark and normal skin regions are included in A and B respectively. The spectra within the black box of the HE staining images were classified by the PLS model, and the prediction results in the electrical mark (C) and normal skin areas (D) were revealed by pseudo-colored images. Electrical epidermis, normal epidermis and normal dermis appear in yellow, light blue and brown respectively.</p

The result of prediction in the validation group by PLS model.

<p>The result of prediction in the validation group by PLS model.</p

The comparison of FTIR mappings based on protein conformations between electrical mark and normal skin.

<p>The first and second rows represent FTIR mappings in the areas of elongation cells and corresponding normal epidermis respectively, in which the peak absorbance intensities associated α-helix, β-turn, β-sheet and antiparallel β-sheet are revealed by “jet” colors with red indicating the highest relative concentration, and blue, the lowest.</p

The results of PLS classification based on spectral dataset in the calibration.

<p>(A) PLS score plot shows that all groups representing different structures of the skin are well-separated along latent factor 1. (B) Prediction result in an additional dataset that are not included for PLS modelling shows that electrical epidermis, normal epidermis and dermis can be distinguishable from each other by using the PLS model. Red and black dot lines show classification threshold for separating the three categories respectively.</p

A comparison of absorbance and second derivative spectra between electrical mark and normal skin.

<p>(A) absorbance spectra within 1800–900 cm^-1 for both categories. Superimposition of them within the Amide I region in absorbance mode (B) and second derivative mode (C). The blue and red spectra represent electrical injury and normal epidermis respectively.</p

Macroscopic and microscopic images of electrical mark and normal skin.

<p>(A) macroscopic features of electrical mark in the hand. Microscopic features of electrical mark (B) and normal skin (C).</p

MTA3 is down-regulated in GEJ adenocarcinoma tissue and in high metastatic potential cancer cell lines.

<p>(A) MTA3 protein is down-regulated in human GEJ adenocarcinoma tissues as determined by immunoblot analysis. N, adjacent noncancerous tissues; C, cancerous tissues. (B) MTA3 transcript is significantly decreased in esophageal and gastric adenocarcinoma tissues, relative to the corresponding normal tissue. The data were from the analysis in Oncomine database. NE, normal esophagus (n = 28); EAC, esophageal adenocarcinoma (n = 75); NG, normal gastric (n = 29); GC, gastric cancer (n = 20). (C) MTA3 mRNA level is decreased in high metastatic potential cell lines of gastric adenocarcinoma and esophageal adenocarcinoma. Gene expression data for MTA3 were extracted from “CCLE Expression Entrez 2012-04-06”. (D) In all 39 tumor cell lines, the exclusively available GEJ adenocarcinoma cell line OE-19 (arrow), which has high metastatic potential, fell into the decreased-MTA3 group composed of 9 cell lines, 7 of which are more invasive. *<i>P</i><0.05, **<i>P</i><0.01, ***<i>P</i><0.001.</p

Survival curves of patients according to expression statues of MTA3.

<p>(A) The OS was significantly better among the patients with MTA3-positive tumors than among the patients with MTA3-negative tumors (<i>P</i><0.001). (B) Among the patients with no lymph node metastasis (N0), the OS was significantly better in the patients with MTA3-positive tumors than in the patients with MTA3-negative tumors (<i>P</i> = 0.018). (C) Among the patients with lymph node metastasis (N1), the OS was significantly better in the patients with MTA3-positive tumors than in the patients with MTA3-negative tumors (<i>P</i> = 0.003). (D) The OS among the patients with MTA3-negative/Snail-positive/E-cadherin-negative tumors was significantly worse than among the patients whose had tumors exhibited other expression patterns (<i>P</i> = 0.003).</p

Univariate and multivariate Cox proportional hazards models showing variables that affect overall survival in patients with GEJ adenocarcinoma.

<p>HR hazard ratio, CI confidence interval.</p

Relationship between MTA3 expression and clinicopathologic variables in tissue samples of GEJ adenocarcinoma (n = 128).

<p>Relationship between MTA3 expression and clinicopathologic variables in tissue samples of GEJ adenocarcinoma (n = 128).</p