Kaplan-Meier analysis of methylation of AR promoter and first exon and overall survival in 208 ccRCC cases (A), or males (B) and females only (C).

<p>The mean methylation level of 11 CpG sites was used. Cases were assigned into methylation high or low group if their methylation level was above or below the median, respectively.</p

List of top 10 genes whose protein expression levels are significantly associated with Time to Death by Cox regression test^a.

<p>List of top 10 genes whose protein expression levels are significantly associated with Time to Death by Cox regression test<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0146505#t001fn001" target="_blank">^a</a>.</p

Pathways enriched in AR-associated genes identified by IPA in ccRCC but not pRCC (A) or vice versa (B).

<p>Pathways enriched in AR-associated genes identified by IPA in ccRCC but not pRCC (A) or vice versa (B).</p

Protein expression of AR and its association with survival in ccRCC.

<p>(A-C) Correlation of AR protein and log2 transformed and mean centered mRNA expression level shown by scatter plot in all cases (A), females (B) and males (C). (D-F) Kaplan-Meier analysis of AR protein expression and overall survival in all cases (D), females (E) and males (F).</p

Box plot of AR protein expression in male and female ccRCC patients.

<p>* indicates statistical significance (p<0.05) by student’s t-test.</p

Hierarchical clustering analysis of AR-associated gene expression and its association with survival in ccRCC.

<p>(A-C, left) The heat map of AR-associated gene expression in all cases (A), females (B) and males (C). Patients were grouped into clusters based on AR-associated gene expression. (A-C, right) Kaplan-Meier analysis of AR-associated gene expression and overall survival in all cases (A), females (B) and males (C).</p

Top 21 pathways enriched in AR-associated genes identified by IPA in ccRCC, pRCC and chRCC.

<p>Red dots label pathways common in ccRCC and pRCC and green dots label pathways common in ccRCC and chRCC. No overlapping was found between pRCC and chRCC.</p

The number of overlapping genes in the 12 common pathways enriched in AR-associated genes between ccRCC and pRCC.

<p>The number of overlapping genes in the 12 common pathways enriched in AR-associated genes between ccRCC and pRCC.</p

Number of AR-associated genes in different subtypes of RCC.

<p>Number of AR-associated genes in different subtypes of RCC.</p

mRNA expression of AR and its association with survival in ccRCC.

<p>(A) Box plot of log2 transformed and mean centered AR mRNA levels in females and males. No significant difference was observed by student’s t-test. (B-D) Kaplan-Meier analysis of AR mRNA expression and overall survival in all cases (B), females (C) and males (D).</p