Gene Conversion Transfers the GAF-A Domain of Phosphodiesterase TbrPDEB1 to One Allele of TbrPDEB2 of Trypanosoma brucei

Cyclic nucleotide specific phosphodiesterases are important regulators of cyclic nucleotide signalling in eukaryotes. In many organisms, including humans and trypanosomes, some of these enzymes contain specific domains (GAF domains) that bind cyclic nucleotides, and that are involved in the regulation of the catalytic domain. In the parasitic protozoon that causes human sleeping sickness, Trypanosoma brucei, two closely related phosphodiesterases each contain two such GAF domains, GAF-A and GAF-B. Their genes are tandemly located on chromosome 9, spaced by only a few thousand nucleotides. We here show that a gene conversion event has exchanged the region that codes for the GAF-A domain of the downstream gene by the closely similar corresponding sequence of the upstream gene. This domain exchange has no effect on intracellular localization of the two enzymes. The gene conversion event has occurred in one particular strain of trypanosomes (Lister427) and is found in all its derivatives, but not in any other strain or isolate. The presence or absence of this gene conversion represents a useful analytical marker for the stringent discrimination of Lister427 derivatives from other trypanosome strains

Disponibilidad, accessibilidad y aplicabilidad de los resultados de investigación de los Centros del Grupo Consultivo para la Investigación Agrícola Internacional (CGIAR)

Presentation: XV Reunión Interamericana de Especialistas en Información Agraria y Medio Ambiental (RIBDA) Lima, Peru, October 27-29, 200

Availability, Accessibility, and Applicability of Research Results of the CGIAR Centers

Presentation: XV Inter-American Meeting of Librarians and Specialists in Agricultural Information (RIBDA) Lima, Peru, October 27-29, 200

Agricultural Information Worldwide, vol. 3, no. 1, 2010

Agricultural Information Worldwide, Volume 3, Number 1, 2010In this issue: FROM THE EDITOR’S DESK / Peter Ballantyne (3); ARTICLES: Agricultural Information and Knowledge Sharing: Promising Opportunities for Agricultural Information Specialists [Article and Abstract], Le partage des informations et de la connaissance agricoles : des occasions prometteuses pour les spécialistes de l'information agricole [Résumé], La información agrícola y el intercambio de conocimientos: Oportunidades promisorias para los especialistas en información agraria [artículo y resumen] / Peter Ballantyne (4); Benchmarking CGIAR research outputs for availability and accessibility [Article and Abstract], Evaluación comparativa de los resultados de investigación del CGIAR en términos de disponibilidad y accesibilidad [resumen], Evaluation de la disponibilité et l'accessibilité des résultats de recherche du GCRAI [Résumé] / Meena Arivananthan, Peter Ballantyne, Enrica M. Porcari (17); Learning to Swim: How IFPRI Uses Multiple Web Channels to Communicate Research Outputs [Article and Abstract], Aprendiendo a Nadar: Cómo el IFPRI utiliza múltiples canales de la Web para comunicar resultados de investigación [Resumen], Apprendre à nager ou comment l’IFPRI utilise les canaux multiples de la toile pour communiquer les résultats de recherche [Résumé] / Chris Addison, Luz Marina Alvaré (23); AGRIS - From a bibliographic database to a semantic data service on agricultural research information [Article and Abstract], AGRIS - De una base de datos bibliográficos a un servicio semántico de datos sobre información agrícola [Resumen], AGRIS - D’une base de données bibliographiques à un service de données sémantiques sur l'information de recherche agricole [Résumé] / Angela Fogarolli, Johannes Keizer, Stefano Anibaldi, Dan Brickley (26); Disponibilidad, accesibilidad y aplicabilidad de los resultados de investigación de los Centros del Grupo Consultivo para la Investigación Agrícola Internacional (CGIAR) [artículo y resumen], Availability, accessibility, and applicability of research results of the CGIAR Centers [Abstract], La disponibilité, l’accessibilité et l’applicabilité des résultats de recherche des centres du Groupe consultatif pour la recherche agricole internationale (GCRAI) [Résumé] / Edith Hesse, Peter Ballantyne, Mariano Mejía (31); VERCON – the Virtual Extension and Research Communication Network [Article and Abstract], VERCON: Red virtual de comunicación sobre la extensión y la investigación [Resumen], VERCON : Réseau virtuel de communication pour la vulgarisation et la recherche [Résumé] / Sophie Treinen (39); Mobile Services in a Wireless World: The CTA 2009 ICT Observatory Meeting [Article and Abstract], Servicios móviles en un mundo inalámbrico: Reunión Observatorio sobre TIC de CTA 2009 [Resumen], Les services d'information mobiles dans un monde sans fil : la réunion 2009 de l'Observatoire du CTA sur les TICs [Résumé] / Pete Cranston, Kevin Painting (44