Foundations in Wisconsin: A Directory [35th ed. 2016]

The 2016 release of Foundations in Wisconsin marks the 35th edition of the print directory and the 16th edition of the online version. The directory is designed as a research tool for grantseekers interested in locating information on private, corporate, and community foundations registered in Wisconsin. Each entry in this new edition has been updated or reviewed to provide the most current information available. Most of the data was drawn from IRS 990-PF tax returns filed by the foundations. Additional information was obtained from surveys, foundation websites, and annual reports. This edition paints a very positive picture of financial growth for Wisconsin foundations. Both grant and asset totals have risen to all-time highs. Of particular note, total grants broke the $600 million barrier, increasing by 8$623 million. Additionally, 58 new foundations have been identified this year. (See page 269 for the complete list.) The following table illustrates the 10-year financial pattern as documented in Foundations in Wisconsin.https://epublications.marquette.edu/lib_fiw/1014/thumbnail.jp

Foundations in Wisconsin: A Directory (37th ed. 2018)

The 2018 edition of Foundations in Wisconsin is the 37th edition of the print directory and the 18th year of the online version. The directory is designed as a research tool for grantseekers interested in locating information on private, corporate, and community foundations registered in Wisconsin. Each entry in this new edition has been updated or reviewed to provide the most current information available. Most of the data was drawn from IRS 990-PF tax returns filed by the foundations. Additional information was obtained from surveys, foundation websites, and annual reports.https://epublications.marquette.edu/lib_fiw/1016/thumbnail.jp

Foundations in Wisconsin: A Directory [36th ed. 2017]

The 2017 edition of Foundations in Wisconsin is the 36th production of the print directory and the 17th year of the web-based database (www.wifoundations.org) The directory is designed as a research tool for grantseekers interested in locating information on private, corporate, and community foundations registered in Wisconsin. Each entry in this new edition has been updated or reviewed to provide the most current information available. Most of the data was drawn from IRS 990-PF tax returns filed by the foundations. Additional information was obtained from surveys, foundation websites, and annual reports.https://epublications.marquette.edu/lib_fiw/1015/thumbnail.jp

Enhanced Detection of Community-Acquired Pneumonia Pathogens With the BioFire® Pneumonia FilmArray® Panel.

Background: Although most observational studies identify viral or bacterial pathogens in 50% or less of patients hospitalized with community-acquired pneumonia (CAP), we previously demonstrated that a multi-test bundle (MTB) detected a potential pathogen in 73% of patients. This study compares detection rates for potential pathogens with the MTB versus the Biofire® Pneumonia FilmArray® panel (BPFA) multiplex PCR platform and presents an approach for integrating BPFA results as a foundation for subsequent antibiotic stewardship (AS) activities. Methods: Between January 2017 to March 2018, all patients admitted for CAP were enrolled. Patients were considered evaluable if all elements of the MTB and the BPFA were completed, and they met other a priori inclusion criteria. The primary endpoint was the percentage of potential pathogens detected using the MTB (8 viral and 6 bacterial targets) versus the BPFA (8 viral and 18 bacterial targets). Blood and sputum cultures were performed on all patients. Two or more procalcitonin (PCT) levels assisted clinical assessments as to whether detected bacteria were invading or colonizing. Results: Of 585 enrolled patients, 274 were evaluable. A potential viral pathogen was detected in 40.5% with MTB versus 60.9% of patients with BPFA with an odds ratio (95% CI) of 9.00 (4.12 to 23.30) p\u3c0.01. A potential bacterial pathogen was identified in 66.4% with the MTB vs 75.5% with the BPFA odds ratio (95% CI) of 2.09 (1.24 to 3.59), p 0.003). Low PCT levels helped identify detected bacteria as colonizers. Keywords: Community-acquired pneumonia; diagnostics; filmarray; pneumonia; procalcitonin

Complete Genome Sequences of Cluster A Mycobacteriophages BobSwaget, Fred313, KADY, Lokk, MyraDee, Stagni, and StepMih

Seven mycobacteriophages from distinct geographical locations were isolated, using Mycobacterium smegmatis mc2155 as the host, and then purified and sequenced. All of the genomes are related to cluster A mycobacteriophages, BobSwaget and Lokk in subcluster A2; Fred313, KADY, Stagni, and StepMih in subcluster A3; and MyraDee in subcluster A18, the first phage to be assigned to that subcluster

Transcriptional and Post-Transcriptional Regulation of SPAST, the Gene Most Frequently Mutated in Hereditary Spastic Paraplegia

Hereditary spastic paraplegias (HSPs) comprise a group of neurodegenerative disorders that are characterized by progressive spasticity of the lower extremities, due to axonal degeneration in the corticospinal motor tracts. HSPs are genetically heterogeneous and show autosomal dominant inheritance in ∼70–80% of cases, with additional cases being recessive or X-linked. The most common type of HSP is SPG4 with mutations in the SPAST gene, encoding spastin, which occurs in 40% of dominantly inherited cases and in ∼10% of sporadic cases. Both loss-of-function and dominant-negative mutation mechanisms have been described for SPG4, suggesting that precise or stoichiometric levels of spastin are necessary for biological function. Therefore, we hypothesized that regulatory mechanisms controlling expression of SPAST are important determinants of spastin biology, and if altered, could contribute to the development and progression of the disease. To examine the transcriptional and post-transcriptional regulation of SPAST, we used molecular phylogenetic methods to identify conserved sequences for putative transcription factor binding sites and miRNA targeting motifs in the SPAST promoter and 3′-UTR, respectively. By a variety of molecular methods, we demonstrate that SPAST transcription is positively regulated by NRF1 and SOX11. Furthermore, we show that miR-96 and miR-182 negatively regulate SPAST by effects on mRNA stability and protein level. These transcriptional and miRNA regulatory mechanisms provide new functional targets for mutation screening and therapeutic targeting in HSP

Genre and Loss: The Impossibility of Restoration in 20th Century Detective Fiction

My project situates loss, rather than restoration, as the identifying trait of the detective fiction genre. I contend that instead of providing a problem-solution model that gives readers closure and reinforces simplified understandings of good and evil, detective fiction refuses to build comforting narratives that rehabilitate a corrupted world. Detective fiction, with its continual attempts to provide an unobtainable solution, ruminates on the impossibility of restoration.Genre and Loss: The Impossibility of Restoration in 20th Century Detective Fiction divides into four chapters, each addressing a perceived subdivision of the detective fiction genre in order to illuminate the unifying connections between them. In each chapter, I pair transatlantic texts in order to highlight the genre’s cohesive, continuing orientation, across nations and time periods, around different enactments of loss. Using Chandler’s The High Window and Sayers’s Busman’s Honeymoon, I begin by contradicting an oft-replicated division drawn between the supposed comfort and security of the British cosies and the corrupt world of the American hard-boileds. Next, I argue that the protagonists of Fleming’s Casino Royale and Thompson’s The Killer Inside Me experience internal loss that prevents them from operating as authoritative producers of solutions. La Bern’s Goodbye Picadilly, Farewell Leicester Square and Highsmith’s The Glass Cell parallel the detective’s loss of individual authority with the inability of the protagonists to prevent the attenuation of control over their own self-identity. Then, I analyze McIlvanney’s Laidlaw and Block’s The Sins of the Fathers to contend that the detectives use their vocation to acknowledge loss as an unavoidable element of life, and to embrace it by prioritizing a continual interrogation of certainty over false closure achieved through criminal convictions. Finally, I conclude the project with a brief exploration of Auster’s City of Glass and Miéville’s The City and The City. These more recent texts highlight the necessity of engaging in ongoing interpretation rather than the possibility of locating a stable answer. Even as the genre of detective fiction develops further, its trajectory continues to trace, and retrace, the steps around the same central theme, the inability to find closure, or an endpoint of restoration