Some hematological and biochemical changes in blood serum of Beluga (Huso huso) after chronic exposure to diazinon

Hematological and biochemical changes were studied in beluga (Huso huso) weighing 450±50g in the presence of b,1-3, D-glucan as a single intraperitoneal injection (0.3mg/kg body weight) once, and thereafter a chronic exposure to organophosphate, diazinon at concentration of 1.5 mg/L as a continuous bath for 63 days at 22±1ºC. Results of erythrocyte profile in fish treated with glucan, but without diazinon, generally showed significant higher values of RBC (P0.05) in the values of HCT, Hb, MCH and MCHC between the two groups. Also, fish treated with glucan, but without diazinon bath, showed significant higher values of RBC than that untreated fish up to 3 weeks post-treatment (P0.05). Furthermore, fish treated with diazinon showed higher levels of alkaline phosphatase (ALP), alanine aminotransferase (ALT), lactate dehydrogenase (LDH) than other three groups for 14-28 days post-treatment and then they decreased to the lower level for the rest of experiment. Also, fish treated with glucan-diazinon showed insignificant higher levels of these enzymes up to 21 days post-exposure compare to fish treated with glucan but without diazinon bath (P>0.05). However, such levels reduced to a lower level in this group for the rest of experiment (P>0.05). Also, fish treated with glucan-diazinon showed generally lower and higher levels of total protein and glucose concentrations in blood plasma, respectively compared to fish treated with glucan but without diazinon bath (P<0.05)

Study of some hematological and biochemical parameters of rainbow trout (Oncorhynchus mykiss) fry in western part of Mazandaran province, Iran

This study was done to investigate of some hematological and biochemical factors of rainbow trout (Oncorhynchus mykiss ) fry in west of Mazandaran in Iran. About 50 pools of blood samples from diseased fry were collected within 30 months from November 2002 till March 2005 from three hatchery farms in western part of Mazandaran province. In addition 30 pools of blood samples as control group were collected randomly from mentioned farms. Each blood samples were examined for whole blood examination and blood enzymes measurement. It consist of total leukocytes (WBC) and erythrocytes counts (RBC), hemoglobin (Hb) content, hematocrits (PCV), leukocytes differential count and blood indices such as MCV, MCH and MCHC. Also blood serums were analysed for total protein (TP) and blood enzymes. All the calculations were made using the SPSS© and t-test statistical method. In hematological findings nine parameters were revealed significant differences (P<0.05) with control group in t-test. It consisted of total WBC, Lymph, Neut, Hb and HCT, MCHC, AST, DL and total protein plasma. Also in total white blood cell count, Lymph and Neut had significant differences as compared with the control fish (P<0.05). Also blood serum components analysis revealed that only LDH and AST amount showed obvious significant differences (P<0.05). Regarding to results it could be concluded that hematological and biochemical studies could be a valuable tool for prognosis and primary diagnosis in some infectious diseases. So it could be recommended for monitoring and surveillance programs in coldwater hatchery health status in Iran

Amiodarone Versus Propafenone to Treat Atrial Fibrillation after Coronary Artery Bypass Grafting: A Randomized Double Blind Controlled Trial

Background: Atrial fibrillation (AF) is one of the most common complications after cardiac surgery. Several therapeutic and preventive strategies have been introduced for postoperative AF, but the treatment and prophylaxis of AF remain controversial. We aimed to compare the efficacy of intravenous amiodarone and oral propafenone in the treatment of AF after coronary artery bypass grafting (CABG). Methods: This was a randomized controlled trial performed in two hospitals in Shiraz, Iran from 2009 to 2012. We included all patients who underwent elective CABG and developed AF postoperatively. The patients were randomly assigned to receive propafenone or amiodarone. The duration of AF, the success rate of the treatment, the need for cardioversion, the frequency of repeated AF, and the need for repeating the treatment were compared. Results: The duration of the first (p=0.361), second (p=0.832), and third (p=0.298) episodes of AF, the need for cardioversion (p=0.998), and the need to repeat the first and second doses of drugs (p=0.557, 0.699) were comparable between the study groups. Repeated AF was observed in 17 patients (30.9%) in the propafenone group and 23 patients (34.3%) in the amiodarone group (p=0.704). Conclusion: Oral propafenone and intravenous amiodarone are equally effective in the treatment and conversion of recent-onset AF after CABG

A New Aprroach for the Assesment of Coronary Artery Disease Risk

Abstract: Coronary artery diseas

An analytic model for a cooperative ballistic deposition in one dimension

We formulate a model for a cooperative ballistic deposition (CBD) process whereby the incoming particles are correlated with the ones already adsorbed via attractive force. The strength of the correlation is controlled by a tunable parameter $a$ that interpolates the classical car parking problem at $a=0$, the ballistic deposition at $a=1$ and the CBD model at $a>1$. The effects of the correlation in the CBD model are as follows. The jamming coverage $q(a)$ increases with the strength of attraction $a$ due to an ever increasing tendency of cluster formation. The system almost reaches the closest packing structure as $a\to\infty$ but never forms a percolating cluster which is typical to 1D system. In the large $a$ regime, the mean cluster size $k$ increases as $a^{1/2}$. Furthermore, the asymptotic approach towards the closest packing is purely algebraic both with $a$ as $q(\infty)-q(a) \sim a^{-1/2}$ and with $k$ as $q(\infty)-q(k) \sim k^{-1}$ where $q(\infty)\simeq 1$.Comment: 9 pages (in Revtex4), 9 eps figures; Submitted to publicatio

Antibiogram of Pseudomonas species: an important tool to combat antibiotic resistance for patient safety in Gombe, Nigeria

Background: Pseudomonas species are responsible for different healthcare-associated infections and are inherently resistant to many commonly used antibiotics. Hospital antibiograms are either absent or not regularly available in most healthcare facilities in Nigeria. The objective of this study is to present the antibiogram of Pseudomonas isolates in Federal Teaching Hospital Gombe (FTHG) in order to guide antibiotic prescription for better patient safety in the hospital.Methodology: The is a hospital-based cross-sectional study. A total of 4309 bacterial isolates were recovered from aerobic cultures of routine clinical specimens including urine, sputum, blood, swabs, aspirates, biopsies, seminal fluids and cerebrospinal fluids at the Medical Microbiology laboratory of the hospital between January and December 2019. Pseudomonas species were identified by colony morphology, Gram-reaction and conventional biochemical tests. Antibiotic susceptibility testing was performed on each Pseudomonas isolate using the modified Kirby-Bauer disk diffusion method on Mueller-Hinton agar and results interpreted according to the guideline of the Clinical and Laboratory Standards Institute (CLSI). Data were analysed using the Statistical Package for Social Sciences (SPSSTM) software version 23.0.Results: Of the total 4309 bacterial isolates, 436 (10.1%) Pseudomonas species were identified, with majority (49.8%) from urine specimens. Antibiotic susceptibility test results revealed average susceptibility rates of 73.8%, 70.1%, 66.2%, 59.5%, and 34.3% to ciprofloxacin, gentamicin, levofloxacin ceftazidime, and carbenicillin respectively. These rates fluctuate only slightly for each of the antibiotic during the 12 months period of survey.Conclusion: Pseudomonas species were most sensitive to ciprofloxacin and gentamicin among the first line antibiotics in FTHG in 2019. Regular updates and presentation of hospital antibiogram especially for intrinsically resistant bacteria such as Pseudomonas involved in healthcare associated infections, is an important tool in combating antimicrobial resistance and ensuring patient safety. Keywords: antibiogram, Pseudomonas, antimicrobial resistance, antimicrobial stewardship, patient safet

Pathogenicity of viral nervous necrosis virus for Guppy fish, Poecilia reticulata

The pathogenicity of a Nervous Necrosis Virus isolate obtained from naturally infected Golden grey mullet (Liza auratus) suffering serious mortalities in Iranian coastline water of the Caspian Sea was investigated for first time. An experimental infection has been performed using three groups, two experimental groups and one control group of Guppy (Poecilia reticulata) with mean weight 0.47±0.09 g, at temperature 25ºC. The infectious dosage (50 ml) with TCID50/ml= 10^4.25 for 2 hours in group 1 and 4 hours in group 2 developed the disease with immersion method. Clear clinical signs associated with significant mortality were observed since 15 dpi. Cumulative mortalities rose to 100% at 30 dpi. While in the control group no mortality was recorded. Virus was re-isolated on SSN-1 cell line that showing typical CPE developed after inoculation with tissues filtrate from dead fish. Histopathological examination of exposed fish, showed clear vacuolization in the granular layer of the retina and cerebellum. TEM micrographs revealed intracytoplasmic vacuoles in the retina of infected Guppy. IHC revealed the presence of viral antigens in the brain and retina. These results confirmed the pathogenicity of the NNV isolate obtained from Golden grey mullet suffering high mortality with regard to suggest that the same agent isolated from golden grey mullet is very likely the cause of the mortality observed in the same species

Some hematological and biochemical changes in blood serum of grass carp (Ctenopharyngodon idella ) vaccinated with Aeromonas hydrophila following exposure to sublethal concentration of diazinon

Diazinon is commonly used for pest control in the agricultural fields in north of Iran. This study was conducted to determine the chronic toxicity of organophosphorous pesticide (Diazinon) in vaccinated fish and its effects on some hematological parameters and biochemical blood plasma profiles of Grass Carp (Ctenopharyngodon idella). This experiment was carried out in three groups. The first group was vaccinated and exposed to diazinon (group A) while the second group was vaccinated and bathed with PBS bath (group B). The remaining fish were used as unvaccinated fish and were kept in clean water separately (group C). Diazinon was applied at concentrations of 2 mg/L for 12 hours since the experiments were initiated. The experimental groups (A and B) showed significantly lower values (p < 0.05) of erythrocyte count, haemoglobin content, haematocrit, leucocytes, Lymphocyte, myelocyte and monocyte, as well as in alkaline phosphatases, alanine aminotransferase, aspartate aminotransferase and, lactate dehydrogenase compared to the control group (C). Values of MCV, MCH and MCHC of experimental species (A and B) were compared to the control group (C). The results of examinations of the biochemical blood plasma profile indicate a marked neurotoxic effect of diazinon in fishes. Changes in values of both erythrocyte and leukocyte profile after exposure to diazinon-based preparation may be referred to disruption of haematopoiesis as well as to a decrease on non-specific immunity of the fish

The Effect of Carbon Black Grades in Tyre Tread Compounds

In this study carbon black filler was added to the various formulations of tyre tread compounds. The carbon black grades chosen were N339/N375 and N550/N660. The compounds were characterised with respect to their rheological and physical properties. It was found that the carbon black grades of N375 and N339 could be substituted readily with minimal effects to the hardness, rebound resilience, tensile and tear strength. On the other hand, the carbon black of N550 could not be directly substituted with N660 as it affects the hardness and resilience of the vulcanizate

Identification of identification of Hepatopancreatic Parvo-like Virus (HPV) in cultured Penaeus semisulcatus from the Islamic Republic of Iran

During the period from August 1997 to March 1998, two thousand samples of cultured Penaeus semisulcatus postlarvae and subadults were collected from five hatcheries and 20 grow out farms distributed in three provinces along the costs of Persian Gulf and the Oman Sea. Based on growth signs, LM and TEM histopathology, Hepatopancreatic Parvo-like Virus (HPV) were identified from the samples. The HPV particles most often appeared in spherical, but occasionally in angular forms. The particles in hepatopancreatocyte sections, averaged 22-24mm in diameter