How do the environmental extremes of Siberian permafrost soils shape the composition of the bacterial soil community?

Microbial communities in permafrost soils of the Siberian Arctic are exposed to extreme environmental conditions. The soils are frozen throughout the entire year except for the short summer period, when thawing of the uppermost 20 to 50 cm of the permafrost sediment allows for the formation of a so-called active layer. Active layers show steep temperature gradients between 10 to 18 °C near the surface and 0 to 1 °C near the permafrost table. Additionally, seasonal freezing and thawing processes lead to the formation of patterns of low-centered polygons. Low-centered polygons determine a pronounced small-scale heterogeneity with regard to their physical and chemical properties between the elevated polygon rims and the depressed polygon centers.Within the active layer of a polygon rim, vertical profiles of potential methane oxidation rates in respond to different temperatures indicated a shift in the temperature optimum from 21 °C near the surface to 4 °C near the permafrost table [1]. This temperature shift could not be shown in samples of the polygon center. Based on these results we used 16S rDNA clone libraries as well as in-situ cell counting to compare the bacterial, in particular the methane oxidizing, community near the surface and near the permafrost table in samples of the polygon rim. The phylogenetic analyses show that the composition of the bacterial community near the surface is significantly different from the bacterial community near the permafrost table. The results also show that bacterial diversity and abundance in Siberian permafrost soils are comparably high as in temperate terrestrial environments.[1] Liebner. S. and Wagner, D. (in press) Abundance, distribution and potential activity of methane oxidizing bacteria in permafrost soils from the Lena Delta, Siberia. Environmental Microbiology doi: 10.1111/j.1462-2920.2006.01120.

Do motorways shape urban growth? Analysis of growth patterns with micro-level data – before and after road openings in two Danish motorway corridors

The paper is an offspring from the Research project Town, Road and Landscape that aims to assess the effect of the Danish motorway network (specifically the last 20 years) on urban growth and interaction patterns. As one of the main interests of the project is the changing urban form and the changing character of the roadscape, the impact of the motorway is in part analysed with micro level data, spatial statistics and GIS – to allowing mapping of changing development trends in motorway corridors. The paper presents analysis of the impact of motorway openings on urban form in two Danish motorway corridors. The analysis is based on a before and after perspective – where the building activity and its location (building register with address coordinates) after the opening of the motorway is compared to building activity in the years before the construction of the motorway. Preliminary results suggest that the motorway most markedly influences the location of non-residential building activities within the city – in favour of locations near the entrance points to/from the motorway network. The development can be explained in part by municipal planning, which in some instances has opened up the new locations for development far ahead of market demand – and in part by an increasing demand for exposed and accessible sites for business development which still seems to be in its beginning.

Graded collector heterojunction bipolar transistor

A graded collector heterojunction bipolar transistor is proposed. The graded collector improves device speed performance at high current densities by reducing the influence of the Kirk effect

Noise equivalent circuit of a semiconductor laser diode

The noise equivalent circuit of a semiconductor laser diode is derived from the rate equations including Langevin noise sources. This equivalent circuit allows a straightforward calculation of the noise and modulation characteristics of a laser diode combined with electronic components. The intrinsic junction voltage noise spectrum and the light intensity fluctuation of a current driven laser diode are calculated as a function of bias current and frequency

Passive mode locking of buried heterostructure lasers with nonuniform current injection

In this letter we report on a novel method to passively mode lock a semiconductor laser. We present experimental results of GaAlAs buried heterostructure semiconductor laser with a split contact coupled to an external cavity. The split contact structure is used to introduce a controllable amount of saturable absorption which is necessary to initiate passive mode locking. Unlike previous passive mode locking techniques, the method presented does not rely on absorption introduced by damaging the crystal and is consequently inherently more reliable. We have obtained pulses with a full width at half-maximum of 35 ps at repetition frequencies between 500 MHz and 1.5 GHz

The intrinsic electrical equivalent circuit of a laser diode

The basic electrical equivalent circuit of a laser diode is derived. The effects of spontaneous emission and self-pulsations are included. It is found that self-pulsations are represented by a negative resistance in the model. Application of this model suggests purely electronic methods of suppressing relaxation oscillations in laser diodes

Monolithic integration of a GaAlAs buried-heterostructure laser and a bipolar phototransistor

A GaAlAs buried-heterostructure laser has been monolithically integrated with a bipolar phototransistor. The heterojunction transistor was formed by the regrowth of the burying layers of the laser. Typical threshold current values for the lasers were 30 mA. Common-emitter current gains for the phototransistor of 100–400 and light responsivity of 75 A/W (for wavelengths of 0.82 µm) at collector current levels of 15 mA were obtained

The oxygen-independent metabolism of cyclic monoterpenes in Castellaniella defragrans 65Phen

BACKGROUND: The facultatively anaerobic betaproteobacterium Castellaniella defragrans 65Phen utilizes acyclic, monocyclic and bicyclic monoterpenes as sole carbon source under oxic as well as anoxic conditions. A biotransformation pathway of the acyclic β-myrcene required linalool dehydratase-isomerase as initial enzyme acting on the hydrocarbon. An in-frame deletion mutant did not use myrcene, but was able to grow on monocyclic monoterpenes. The genome sequence and a comparative proteome analysis together with a random transposon mutagenesis were conducted to identify genes involved in the monocyclic monoterpene metabolism. Metabolites accumulating in cultures of transposon and in-frame deletion mutants disclosed the degradation pathway. RESULTS: Castellaniella defragrans 65Phen oxidizes the monocyclic monoterpene limonene at the primary methyl group forming perillyl alcohol. The genome of 3.95 Mb contained a 70 kb genome island coding for over 50 proteins involved in the monoterpene metabolism. This island showed higher homology to genes of another monoterpene-mineralizing betaproteobacterium, Thauera terpenica 58Eu(T), than to genomes of the family Alcaligenaceae, which harbors the genus Castellaniella. A collection of 72 transposon mutants unable to grow on limonene contained 17 inactivated genes, with 46 mutants located in the two genes ctmAB (cyclic terpene metabolism). CtmA and ctmB were annotated as FAD-dependent oxidoreductases and clustered together with ctmE, a 2Fe-2S ferredoxin gene, and ctmF, coding for a NADH:ferredoxin oxidoreductase. Transposon mutants of ctmA, B or E did not grow aerobically or anaerobically on limonene, but on perillyl alcohol. The next steps in the pathway are catalyzed by the geraniol dehydrogenase GeoA and the geranial dehydrogenase GeoB, yielding perillic acid. Two transposon mutants had inactivated genes of the monoterpene ring cleavage (mrc) pathway. 2-Methylcitrate synthase and 2-methylcitrate dehydratase were also essential for the monoterpene metabolism but not for growth on acetate. CONCLUSIONS: The genome of Castellaniella defragrans 65Phen is related to other genomes of Alcaligenaceae, but contains a genomic island with genes of the monoterpene metabolism. Castellaniella defragrans 65Phen degrades limonene via a limonene dehydrogenase and the oxidation of perillyl alcohol. The initial oxidation at the primary methyl group is independent of molecular oxygen

A Framework For MIS Student Outcome Assessment And Program Review

Business schools and programs are under increasing pressure from accrediting bodies to develop and implement a cohesive, documented, and theoretically sound method for assessing student outcomes (Michlitsch and Sidle, 2002).  For schools accredited by Association to Advance Collegiate Schools of Business, outcomes assessment and review is an integral component of the continuous improvement standard (AACSB, 2003).  This paper outlines the development of a systematic and practical methodology for assessing program effectiveness and monitoring student development in the undergraduate MIS program.  The model is a result of multiple needs identified during curriculum revitalization sessions and is responsive to additional issues relating to program assessment review, institutional effectiveness, and a student electronic portfolio project