Lectin histochemistry of posterior lingual glands of developing rats

The posterior lingual glands are classified as Weber and von Ebner glands. Glycans play an important role in salivary glands. Although the distribution of glycans can explain functional diversity and variation, there are many unknowns in the developing rat posterior lingual glands. The purpose of this study was to elucidate the relationship between the development and function of the posterior lingual gland in rats by histochemical analysis using lectins that bind to sugar residues. In adult rats, Arachis hypogaea (PNA), Glycine maximus (SBA), and Triticum vulgaris (WGA) were associated with serous cells and Dolichos biflorus (DBA) with mucous cells. In both Weber's and von Ebner's glands, all 4 lectins were bound to serous cells in early development, but as development progressed, DBA disappeared in serous cells and only the DBA remained in mucous cells. These results suggest that Galβ (1,3) > Galβ(1,4) > Gal, αGalNAc > αGal > βGalNAc, NeuAc > (GalNAc)2–3>>>GlcNAc, and GalNAcα(1,3) are present in the early stage of development, but that GalNAcα(1,3) disappear in serous cells and only GalNAcα(1,3) are localized in mucous cells after maturation. These results indicate that Weber glands function as serous glands in the early postnatal stage when von Ebner glands have not matured.Harada K., Miki K., Tanaka S., et al. Lectin histochemistry of posterior lingual glands of developing rats. Scientific Reports 13, 10365 (2023); https://doi.org/10.1038/s41598-023-36154-9

Photoresponse in the Ciliated Protozoan Colpoda cucullus

Kawano, Noriyuki, Funadani, Ryoji, Arikawa, Mikihiko, Harada, Tetsuo, Suizu, Futoshi, Matsuoka, Kou, Matsuoka, Tatsuomi (2017): Photoresponse in the Ciliated Protozoan Colpoda cucullus. Acta Protozoologica 56 (1): 1-7, DOI: 10.4467/16890027AP.17.001.6965, URL: https://www.mendeley.com/catalogue/0cd0b706-68e1-3ba9-9e25-c545a0f41b65

松山市東方地域の和泉層群の研究

The lithological aspects of the Izumi group, the notes of general geology and the structural geology are described. The elongated area of Northern Shikoku is covered by the marine elastic sediments, the upper Cretaceous Izumi Group, which is distributed along the Median Tectonic Line of the Southwest Japan. They consist of a large volume of elastics. The stratigraphic succession of the Izumi Group in the investigated area is summarized as follows in ascending order. / Lower subgroup / Formation A : conglomerates and sandstones / Formation B: sandstones with thin shales / Formation C : alternation of shales and sandstones / Middle subgroup / Formation D: alternation of sandstones and shales lower part predominated sandstones / Formation E : shales alternated with sandstones / Upper subgroup / Formation F: chiefly-coarse grained sandstones, partly with sporadic gravels / Formation G: alternation of sandstones and shales / Formation H: conglomeratic sandstones One subgroup shows one sedimentary cycle, the lower part of which is dominated by coarse-grained elastic rocks. Various types of graded bedding are shown in Figs. 3 to 6 and Tables 1 to 7. The direction of the sedimentary transport is determined as being from northeast to southwest by the analysis of primary sedimentary structures. As a result of the comparison of the sedimentary environments, the main part of them are considered to be a kind of submarine fan

Fig. 3 in Photoresponse in the Ciliated Protozoan Colpoda cucullus

Fig. 3. Photoresponse of C. cucullus Nag-1 to an increase or decrease in white light intensity. (a) Side-view of a chamber to observe the photoresponse of the cells. The cell suspension was kept 1-mm thick. (b) The temperature of the cell suspension after stimulation light was applied (arrow). (c) Directional changes (photophobic response) of the cells to a sudden increase (c-1) or decrease (c-2) in white light intensity. Prior to "ON" and "OFF" stimulation, the cells were adapted for 1 min in the background dim-light (0.65 W·m–2) and bright light (7 W·m–2), respectively. The cells responded by turning more than 90 degrees in 2 s after light stimulation was expressed as the percentage of the total number of tested cells (100 cells). (d) Alteration in steady-state forward swimming velocity of the cells induced by continuous light stimulation (photokinetic response). Prior to "ON" and "OFF" stimulation, the cells were adapted for 1 min in dim-light (0.65 W·m–2) and bright light (7 W·m–2), respectively. (d-1) Time course of forward swimming velocity after the onset of step-up light stimulation. The shaded circles indicate the swimming velocity at 1 s before light intensity was increased. Asterisks indicate significant differences in the swimming velocity before light stimulation (shaded circle) (p <0.05, Mann-Whitney test). (d-2) Time course of forward swimming velocity after the onset of step-down light stimulation. The open circle is swimming velocity at 1 s before light intensity is decreased.Published as part of Kawano, Noriyuki, Funadani, Ryoji, Arikawa, Mikihiko, Harada, Tetsuo, Suizu, Futoshi, Matsuoka, Kou & Matsuoka, Tatsuomi, 2017, Photoresponse in the Ciliated Protozoan Colpoda cucullus, pp. 1-7 in Acta Protozoologica 56 (1) on page 5, DOI: 10.4467/16890027AP.17.001.6965, http://zenodo.org/record/835688