6 research outputs found
Additional file 1 of Validation of the Meet-URO score in metastatic clear cell renal cell carcinoma patients receiving second or third-line tyrosine kinase inhibitors-immune checkpoint inhibitors combination therapy
Additional file 1: Table S1. Summary of application of Meet-URO score in mRCC patients receiving systemic therapies
Rapid and Selective Detection of Pathogenic Bacteria in Bloodstream Infections with Aptamer-Based Recognition
Sepsis
and bacteremia are life-threatening clinical syndromes associated
with significant patient morbidity and mortality. Rapid and sensitive
detection of pathogenic bacteria is the key to improve patient survival
rates. Herein, we have rationally constructed a simple aptamer-based
capture platform to shorten the time needed for confirmation of bacterial
bloodstream infection in clinical blood samples. This capture platform
is made of a mesoporous TiO<sub>2</sub>-coated magnetic nanoparticle
and is modified with target aptamer. It features excellent bacterial
enrichment efficiency of about 80% even at low bacterial concentrations
(10–2000 CFU mL<sup>–1</sup>). More importantly, the
bacteria can be enriched within 2 h, and the time for bacterial identification
is effectively shortened in comparison to the “gold standard”
in clinical diagnosis of bloodstream infection. The aptamer-based
capture platform may pave a way for the detection of biomarkers and
find potential applications in disease diagnosis
Autofluorescence-Free Targeted Tumor Imaging Based on Luminous Nanoparticles with Composition-Dependent Size and Persistent Luminescence
Optical bioimaging is an indispensable
tool in modern biology and medicine, but the technique is susceptible
to autofluorescence interference. Persistent nanophosphors provide
an easy-to-perform and highly efficient means to eliminate tissue
autofluorescence. However, direct synthesis of persistent nanophosphors
with tunable properties to meet different bioimaging requirements
remains largely unexplored. In this work, zinc gallogermanate (Zn<sub>1+<i>x</i></sub>Ga<sub>2–2<i>x</i></sub>Ge<sub><i>x</i></sub>O<sub>4</sub>:Cr, 0 ≤ <i>x</i> ≤ 0.5, ZGGO:Cr) persistent luminescence nanoparticles
with composition-dependent size and persistent luminescence are reported.
The size of the ZGGO:Cr nanoparticles gradually increases with the
increase of <i>x</i> in the chemical formula. Moreover,
the intensity and decay time of persistent luminescence in ZGGO:Cr
nanoparticles can also be fine-tuned by simply changing <i>x</i> in the formula. <i>In vivo</i> bioimaging tests demonstrate
that ZGGO:Cr nanoparticles can efficiently eliminate tissue autofluorescence,
and the nanoparticles also show good promise in long-term bioimaging
as they can be easily reactivated <i>in vivo</i>. Furthermore,
an aptamer-guided ZGGO:Cr bioprobe is constructed, and it displays
excellent tumor-specific accumulation. The ZGGO:Cr nanoparticles are
ideal for autofluorescence-free targeted bioimaging, indicating their
great potential in monitoring cellular networks and construction of
guiding systems for surgery
One-Dimensional Luminous Nanorods Featuring Tunable Persistent Luminescence for Autofluorescence-Free Biosensing
Persistent luminescence
nanoparticles (PLNPs), which can remain
luminescent after cessation of excitation, have emerged as important
materials in biomedicine due to their special ability to eliminate
tissue autofluorescence. Even though significant advances have been
made in bioimaging, studies on controlled synthesis of PLNPs with
tunable properties are lacking. Until now, only a few studies have
reported the synthesis of quasi-spherical ZnGa<sub>2</sub>O<sub>4</sub>:Cr PLNPs, and direct synthesis of PLNPs with other shapes and chemical
compositions has not been reported. Herein, we report the direct synthesis
of Zn<sub>2</sub>GeO<sub>4</sub>:Mn (ZGO:Mn) persistent luminescence
nanorods (NRs). The length and persistent luminescence of ZGO:Mn NRs
can be fine-tuned by simply changing the pH of the hydrothermal reaction
system. Moreover, ZGO:Mn NRs exhibit rapid growth rate, and NRs with
strong persistent luminescence can be obtained within 30 min of hydrothermal
treatment. Aptamer-guided ZGO:Mn bioprobes were further constructed
and applied to serum lysozyme analysis. Serum samples from patients
with lung cancer, gastric cancer, and colorectal cancer were collected,
and the concentrations of lysozyme in these samples were determined.
Since the bioprobes displayed long persistent luminescence, serum
autofluorescence interference was completely eliminated. The lysozyme
quantification results were in good agreement with those obtained
using a clinical method, suggesting the good potential of the bioprobes
in the analysis of clinical samples. The developed ZGO:Mn NRs possess
tunable length and persistent luminescence, and they are ideal for
eliminating autofluorescence interference in biosensing, making them
valuable in research areas such as studying the functions of biomolecules
and monitoring of molecular/cellular networks in their native contexts
An Ultrasensitive Diagnostic Biochip Based on Biomimetic Periodic Nanostructure-Assisted Rolling Circle Amplification
Developing
portable and sensitive devices for point of care detection
of low abundance bioactive molecules is highly valuable in early diagnosis
of disease. Herein, an ultrasensitive photonic crystals-assisted rolling
circle amplification (PCs-RCA) biochip was constructed and further
applied to circulating microRNAs (miRNAs) detection in serum. The
biochip integrated the optical signal enhancement capability of biomimetic
PCs surface with the thousand-fold signal amplification feature of
RCA. The biomimetic PCs displayed periodic dielectric nanostructure
and significantly enhanced the signal intensity of RCA reaction, leading
to efficient improvement of detection sensitivity. A limit of detection
(LOD) as low as 0.7 aM was obtained on the PCs-RCA biochip, and the
LOD was 7 orders of magnitude lower than that of standard RCA. Moreover,
the PCs-RCA biochip could discriminate a single base variation in
miRNAs. Accurate quantification of ultralow-abundance circulating
miRNAs in clinical serum samples was further achieved with the PCs-RCA
biochip, and patients with the nonsmall cell lung carcinoma were successfully
distinguished from healthy donors. The PCs-RCA biochip can detect
bioactive molecules with ultrahigh sensitivity and good specificity,
making it valuable in clinical disease diagnosis and health assessment
Time-Gated Imaging of Latent Fingerprints and Specific Visualization of Protein Secretions via Molecular Recognition
Persistent
nanophosphors can remain luminescent after excitation
ceases; thus, they offer a promising way to avoid background fluorescence
interference in bioimaging. In this work, Zn<sub>2</sub>GeO<sub>4</sub>:Ga,Mn (ZGO:Ga,Mn) persistent luminescence nanoparticles were developed
and they were employed for time-gated imaging of latent fingerprints
(LFP). The nanoparticles were functionalized with a carboxyl group
and utilized to label LFP through reacting with the amino group in
the LFP. Results proved the potent ability of ZGO:Ga,Mn in eliminating
background fluorescence to afford highly sensitive LFP imaging. Moreover,
LFP aged for 60 days were successfully detected due to the presence
of highly stable amino acids. After being functionalized with concanavalin
A, the nanoparticles achieved visualization of glycoproteins in LFP.
This strategy provides great versatility in LFP imaging and good potential
in uncovering the chemical information within LFP, making it valuable
in forensic investigations and medical diagnostics